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psillypsally
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Registered: 02/29/20
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another white mold or hyphae question
#26510402 - 02/29/20 09:03 PM (3 years, 10 months ago) |
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the jar in question was inoculated on the 21st. The first 2 pictures are 5 days in. I messed up and used a heating pad for incubation. the build-up of moisture leads me to believe its white mold. the rest of the pictures are 8 days after inoculation. the location is above the inoculation hole. tilted the jar a bit to move some grains around and noticed 5 rye grains are clumped together which makes me hope that its a sign of mycelium. would white mold break and tear apart more easily if you move it around? My other jars show no sign of growth or contamination yet altho my temps are at 65F and 70F if I'm lucky. it's probably too early to tell. guess il just keep waiting and see if the growth is an ugly chaotic grey rather than an amazingly sexy bright white with a beautifully spreading hyphae
      
Edited by psillypsally (02/29/20 09:29 PM)
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susurrador
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Re: another white mold or hyphae question [Re: psillypsally]
#26513878 - 03/03/20 02:08 AM (3 years, 10 months ago) |
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If you had hydrated grain jars on a heating pad for any length of time, the moisture buildup is water that has evaporated from the grain then condensed on the glass.
To me it looks like fluffy or tomentose cube mycelium.
You don't want to disturb it as those tiny filaments sever very easily. Let it grow out awhile and post more pics.
-------------------- "If it doesn't work, you can always hit him with it."
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psillypsally
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Re: another white mold or hyphae question [Re: susurrador]
#26519269 - 03/05/20 06:20 PM (3 years, 10 months ago) |
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Quote:
susurrador said: If you had hydrated grain jars on a heating pad for any length of time, the moisture buildup is water that has evaporated from the grain then condensed on the glass.
I know. I shouldn't have used a heatpad, the moisture could have lead to a contam but I'm stuck at 66 degrees F and im too cheap to go buy a space heater. Plus I don't want a lot of air movement in the closet, lol, another reason why im using 1/2 pints jars for grains. I'm too cheap to buy a new PC. all I had was a small 5 liter that can hold 5 1/2pints.
Quote:
To me it looks like fluffy or tomentose cube mycelium.
yea its mycelium. waited impatiently for another 2 days and it grew into beautiful white color. a second jar sprang to life too so that's good news.
Quote:
You don't want to disturb it as those tiny filaments sever very easily. Let it grow out awhile and post more pics.
sounds good. il post an update when it grows a bit
even tho I'm trying to be cheap I just bought some quart jars and ordered a used PC from amazon the Mirro 92122A 22 quart for $56, but the PSI weights didn't come with. need to return it and either buy a new one or buy a Presto 01781 23-Quart. Does anyone have any experience with any of those 2 or anyone knows a better PC other than an all American.
Edited by psillypsally (03/05/20 07:11 PM)
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susurrador
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Re: another white mold or hyphae question [Re: psillypsally]
#26519883 - 03/06/20 01:49 AM (3 years, 10 months ago) |
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The Presto 23 is great. I own 2.
Don't store the lid for it in your oven.
-------------------- "If it doesn't work, you can always hit him with it."
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psillypsally
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Re: another white mold or hyphae question [Re: susurrador]
#26523738 - 03/08/20 11:02 AM (3 years, 10 months ago) |
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So far so good. 3 out of 5 jars are growing so far. Inoculated on the 2/21 with Porte Rican, but I have 4 inoculated on 2/19 with z-strain which show no sign of growth. I might have overdone it with the coffee to water ratio Used 1/2cc per 1/2pint jar and my low temps are not helping either.
Getting another batch of rye grains ready to PC in quart jars with a 24-hour soak and had a couple of questions. Would a 12-hour soak be enough to germinate endospores or would you wait a full 24 hours?
I'm about to run out of the organic rye grain $25 10-lbs on amazon and was trying to find some cheap organic white millet. It's a bit pricey for 50lbs bag at $70-$100. So I guess il go with wild bird seed, but my only concern is fungicides.
Assuming all WBS has been treated with fungicides, do you guys give it an extra wash and rinse before and after a soak or after a simmer? Or do you guys avoid any grains that have been treated with fungicides all together?
the last question was is 3 layers of paper micropore tape too much as a filter for GE?
 
Edited by psillypsally (03/08/20 11:05 AM)
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susurrador
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Re: another white mold or hyphae question [Re: psillypsally]
#26523894 - 03/08/20 12:45 PM (3 years, 10 months ago) |
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I don't know the answer to your endospore question regarding rye, but have seen it discussed in other threads. I've only used whole oats so far and what I've found is if the PC cycle is properly vented and of adequate length- endospores are a non-issue and do not need to be germinated, softened, soaked, etc because the PC cycle will kill them.
Type it into the search tool and click the Trusted cultivator box to pull up posts from trusted shroomery shroom growers. Make sure you're looking at the dates of posts here because this forum has been active 20+ years and a lot has improved in the knowledge and teks during that time.
Do the same for your fungicide question and I'm sure you'll find answers.
-------------------- "If it doesn't work, you can always hit him with it."
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psillypsally
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Re: another white mold or hyphae question [Re: susurrador]
#26541246 - 03/17/20 05:48 PM (3 years, 10 months ago) |
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I have 4 jars 1/2 pint jars of grain inoculated with 1/2cc of spores. Its been 27 days with no growth and no contams. grains look hydrated bit on the dry side followed RRs way. might have fucked it up on the coffee soak but i didn't go crazy strong with the coffee soak. might have been the PC, used a small 5 liter PC. the second batch of rye grains in the 1/2 pints i used a bit less of a coffee soak. and 3 of the 5 spawned. I'm stumped....... just toss the grains?
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IDX Carnage
Sir


Registered: 03/12/20
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Re: another white mold or hyphae question [Re: psillypsally]
#26542234 - 03/18/20 08:22 AM (3 years, 10 months ago) |
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You could leave them in a closet and see what happens, or a suit case. But yea 27 days is a long time.
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