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Offlinepsycho_nauticus
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Swabbing a print
    #26509852 - 02/29/20 02:33 PM (3 years, 10 months ago)

I have a bunch of sterile curios, and I want to inoculate agar via maybe T streak or may just zig zagging some lines. Do I literally just take dry my cutip and roll it over the print on the foil, and then directly streak the agar? Or do I need to wet the cutip or anything? I was just going to do it dry, rub it against the foil, and then streak the agar.


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OfflineKmacmo
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Re: Swabbing a print [Re: psycho_nauticus]
    #26509860 - 02/29/20 02:44 PM (3 years, 10 months ago)

Dry is fine as long as its sterile the spores will still stick to it nicely.
If it's a dark print just pick a spot jab the qtip on the foil and twist then pull back you should see plenty of spores on the end of it.

Wet is fine also as long as the waters sterile, like a spore Syringe just squirt few drops onto qtip and then inoculate plate.


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OfflineCrackatoa
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Re: Swabbing a print [Re: psycho_nauticus] * 1
    #26509861 - 02/29/20 02:45 PM (3 years, 10 months ago)

Are the swabs sterile? if using Q tips you need to PC first. If they are sterile open package, roll swab in print and swipe away


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: Crackatoa] * 1
    #26509869 - 02/29/20 02:55 PM (3 years, 10 months ago)

Yes they are sterile. I have everything sterile still in the package. So I I can do it all dry? Just rub and circle the cutip in foil and then streak plate? Should I expect it to feel really stick then on the agar?

Do I need to rub it on the agar first to get it sticky and then roll the cutip in the foil? Or I can just go straight to foil dry?


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OfflineCrackatoa
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Re: Swabbing a print [Re: psycho_nauticus]
    #26509880 - 02/29/20 03:04 PM (3 years, 10 months ago)

Straight to foil dry, swirl Q tip to get some on it. Don't waste the whole print, it only takes a little. But you should see some spores on the agar. It doesn't take a lot of pressure to destroy the agar depending on hardness of plate. the plate will feel smooth to the q tip as well. I swirl the q tip as I swipe, Sometimes I zip zag, sometimes I just lines, sometimes I put dots.


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OfflineA.k.aM
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Re: Swabbing a print [Re: Crackatoa]
    #26509910 - 02/29/20 03:39 PM (3 years, 10 months ago)

Yeah I definitely split the agar my first time.

Use just enough pressure to keep the swab on the surface while you drag it.

Which print(s) are you going with?


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OfflineLogicaL ChaosM
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Re: Swabbing a print [Re: psycho_nauticus]
    #26509983 - 02/29/20 04:23 PM (3 years, 10 months ago)

Yeah, just swipe with a dry sterile q-tip and yer good to go :thumbup:


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: Crackatoa]
    #26510019 - 02/29/20 04:49 PM (3 years, 10 months ago)

Yeah they're sterile. Do you recommend any type of streak technique? Or just zig zag?


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26510022 - 02/29/20 04:51 PM (3 years, 10 months ago)

Albino A+ is calling my name. They look so bad ass and trippy and look fucking cool. I would love to have an experience with those :smile:


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OfflineLogicaL ChaosM
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Re: Swabbing a print [Re: psycho_nauticus]
    #26510028 - 02/29/20 04:54 PM (3 years, 10 months ago)

Id recommend a simple straight-line streak but that's up to your personal preference.


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: LogicaL Chaos]
    #26523758 - 03/08/20 11:13 AM (3 years, 10 months ago)

I did a zig zag streak, this was about 3 days ago and no germination. Now I'm concerned if I didn't get enough spores from the print or not. I covered the tip of the cutip from the print, and when I streaked there was only some black in the first and second lines. The other lines you can see marked in on the agar, but no spores. But I'm assuming they're there, just microscopic... I thought I would of seen some type of germination by now, I thought prints germinate quicker than a drop of spore syringe solution.


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OfflineCrackatoa
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Re: Swabbing a print [Re: psycho_nauticus]
    #26523779 - 03/08/20 11:30 AM (3 years, 10 months ago)

Spores on a print are dry, they will rehydrate on the agar. print to agar can take up to 2-3 weeks sometimes. Spore syringe will generally go a little quicker.


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: Crackatoa]
    #26523833 - 03/08/20 12:02 PM (3 years, 10 months ago)

Oh I thought it was the other way around.


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Re: Swabbing a print [Re: psycho_nauticus]
    #26523933 - 03/08/20 01:13 PM (3 years, 10 months ago)

What type of swabs are you using? Amazon and you can get medical grade EEOG sterlized cotton swabs cheep. I use them for PE varieties.


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: Sockadin]
    #26524071 - 03/08/20 02:50 PM (3 years, 10 months ago)

Medical sterile. So why do you think? Normal to take longer? I’m thinking that I should have gotten more spores in my swab maybe...?


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OfflineA.k.aM
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Re: Swabbing a print [Re: psycho_nauticus]
    #26524122 - 03/08/20 03:25 PM (3 years, 10 months ago)

That’s the worst part about spore plates. You never know wtf is going on and feel like maybe somehow none of those millions of spores made it to the agar lol.

How many plates did you swipe?? 3 days would be like the absolute earliest unless they’re straight off the cap fresh.


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26524184 - 03/08/20 03:57 PM (3 years, 10 months ago)

I swiped one plate.


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26526024 - 03/09/20 07:23 PM (3 years, 10 months ago)

I'm trying to keep myself busy with getting other supplies and thinking of other things to get down the road while I'm still waiting for this AA+ that I swiped. I don't know what to do in the meantime it's killing me!!! Should I of swiped more than one plate of AA+ and swiped a couple plates of AA+? Or do you think this is normal for time? I see some spores that I got on there from streaking, but not every line is black. I have two faded black lines, and the other lines you can't see anything, just the indentation of my swipe...


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Invisiblewoofwoof
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Re: Swabbing a print [Re: psycho_nauticus]
    #26526044 - 03/09/20 07:38 PM (3 years, 10 months ago)

I swabbed some rusty whytes from spores to agar and it took about 4 days to see any growth.

If you have any spores left over, make a syringe, make agar, do somehting mycology related to keep your mind off of it. I know that feeling!


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OfflineCrackatoa
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Re: Swabbing a print [Re: psycho_nauticus]
    #26526058 - 03/09/20 07:51 PM (3 years, 10 months ago)

Sometimes you don't always see them, they're there. I cant say that I wouldn't have swiped 2-3 but I do singles more often than not. How fresh is the print?


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OfflineA.k.aM
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Re: Swabbing a print [Re: Crackatoa]
    #26526086 - 03/09/20 08:08 PM (3 years, 10 months ago)

I used the same print for lagm and had growth within a week.


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26526093 - 03/09/20 08:11 PM (3 years, 10 months ago)

Oh wow, makes me feel like I did something wrong...Maybe I should of put more on the plate? Temperature is my room temperature, low 70's.


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: woofwoof]
    #26526099 - 03/09/20 08:16 PM (3 years, 10 months ago)

Believe me, I have been...



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Invisiblewoofwoof
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Re: Swabbing a print [Re: psycho_nauticus]
    #26526114 - 03/09/20 08:27 PM (3 years, 10 months ago)

As long as you don't see contams your good! I would suggest to be patient. I bet you there are spores on there! Just let em make love and germinate


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OfflineA.k.aM
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Re: Swabbing a print [Re: woofwoof]
    #26526252 - 03/09/20 09:56 PM (3 years, 10 months ago)

You could always restreak them after a week or whatever. Based on my very limited experience with spore plates if they’re gonna germinate it’ll be happening by a week. I’d say something like 95% of my plates either germinated in a week or still had no growth at a month.


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OfflineCootermonkey
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Re: Swabbing a print [Re: A.k.a]
    #26526623 - 03/10/20 04:59 AM (3 years, 10 months ago)

Heres a little confirmation that you got more than enough spores on it im sure. Its purty cool how many are actually there haha
https://www.shroomery.org/forums/showflat.php/Number/21365919#21365919

And all it takes is 2 to come together


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MyLAGM2020Growlog


Edited by Cootermonkey (03/10/20 05:02 AM)


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26527131 - 03/10/20 11:40 AM (3 years, 10 months ago)

AKA we have germination!!! I can see visible germination in only one spot of my streak. That also happens to be the very first point the cutip touched when I started the streak which has the most spores. When would be the point to transfer from a germination?


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OfflineA.k.aM
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Re: Swabbing a print [Re: psycho_nauticus]
    #26527144 - 03/10/20 11:50 AM (3 years, 10 months ago)

Yeeeeeaaahhh boooyyyeee!!

Lol I usually see the first growth in that spot too, or if I do zig zags a lot of times the corners go first.

Give it a day or two and see how it looks, maybe take a tiny tiny transfer.


How many days was it? I went and looked through my lagm journal and it doesn’t say exactly but it was either day 3 or 4 for me.


Ahh such a relief right.


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Edited by A.k.a (03/10/20 11:50 AM)


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26527166 - 03/10/20 12:01 PM (3 years, 10 months ago)

It was exactly one week. I did a zig zag streak also with the cutip, but I should of swirled the cutip around as I did the streak because there was still a good amount on the sides of the cutip. That's the strongest deposit of spores at the very beginning and since I just used the tip of the cutip, it spread thin, looking like nothing. I have some other black spots at the corners of the zig zag, VERY faint, nowhere how it looked when the cutip first touch. I wonder if those other faint black spots will germinate also, but I'm not holding my breath for it because this should be good enough right? So when I take a transfer am I supposed to avoid the actual spores themselves? That means it would have to grow out pretty wide for me to get a transfer?


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OfflineA.k.aM
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Re: Swabbing a print [Re: psycho_nauticus]
    #26527187 - 03/10/20 12:19 PM (3 years, 10 months ago)

Don’t worry about spores when you transfer, they’re all over but I think usually by the time you’re taking transfers they’ll have grown if they’re gonna.

A bunch of my plates ended up getting growth along the entire swipe pattern, but usually it starts at the very first contact spot and the corners.

I did swipe a print recently to two plates and only had germination in one spot which is lame. But better than nothing!

If you get more they seem usually grow in little contained colonies that wall themselves off when they hit each other like this. This is about when I took transfers, the growth spots are big enough to see good spots and get clean wedges.



But with aa+ it was just a giant thick tangle of myc. Might actually be better if you have less germination in that aspect.


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Edited by A.k.a (03/10/20 12:21 PM)


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26527216 - 03/10/20 12:33 PM (3 years, 10 months ago)

Well hopefully I get a couple more spots of germination...

Hopefully my one spot germinates that well!


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OfflineCrackatoa
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Re: Swabbing a print [Re: psycho_nauticus]
    #26527221 - 03/10/20 12:34 PM (3 years, 10 months ago)

You will:rockon:


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26530833 - 03/12/20 11:39 AM (3 years, 10 months ago)

So what should I be transferring from this germination plate of AA? It’s just a big ball of white over the black spores, and other spots of the streak are starting to germinate also. Do I cut a piece of mycelium that still has black spores in it from the streak? Or do I need to wait for clear margins as usual and only take mycelium?


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OfflineCrackatoa
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Re: Swabbing a print [Re: psycho_nauticus]
    #26530837 - 03/12/20 11:41 AM (3 years, 10 months ago)

Picture?


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26534041 - 03/14/20 06:56 AM (3 years, 10 months ago)

Dude you weren't joking about AA+ being a tangled web of mycelium. I took two transfers once they germinated, and they're like two big poof balls of whiteness. It's starting to grow out a little on the agar, but about how long or how many transfers until good growth? Or is it always like this? Should it spread and colonize the agar faster since I used your agar recipe? (Remember I did all the rest of my plates 7 grams agar/10 LME, does that affect it somehow?)


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OfflineRandalf the Grey
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Re: Swabbing a print [Re: psycho_nauticus]
    #26534180 - 03/14/20 08:26 AM (3 years, 10 months ago)

I never played with AA+ on agar enough to really isolate anything but yeah it has ways looked super white and puffy for me. Everytime I grew them out they just looked different than my other cubes.
These are two germ plates for my current restart. Old dirty prints so still need cleaning but definitely has that puffy growths



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Randalf the Grey
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Re: Swabbing a print [Re: Randalf the Grey]
    #26534183 - 03/14/20 08:29 AM (3 years, 10 months ago)

Mine straightened up on t3. I’ve got a t5 now that’s just thin flat rhizo.

But yeah it was totally different from all the other cubes, my germ plate I thought it was gonna hit the lid.


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Offlinepsycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
    #26534198 - 03/14/20 08:40 AM (3 years, 10 months ago)

Yeah I'm wondering when it's going to stop poofing up in a big ball and I can get some flat growth on agar to put to grain. I haven't been able to even put any agar to grain yet! Is it just going to keep doing that and not spread and colonize the agar? Or just keep taking transfers from the edge and I'll get some flat rhizo growth?


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OfflineA.k.aM
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Re: Swabbing a print [Re: psycho_nauticus]
    #26534272 - 03/14/20 09:19 AM (3 years, 10 months ago)

It’ll spread.

You can definitely put the crazy fluffy growth to grain no problem as long as it’s clean.

The more transfers/isolation you do the more uniform it should get. But also less diversity.


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Re: Swabbing a print [Re: A.k.a]
    #26534320 - 03/14/20 09:38 AM (3 years, 10 months ago)

It's funny I caught this thread just now. I put fluffy stuff to grain more than once and as A.k.a. (I think it was, didn't look at name) said as long as it's clean your good. However, right after I post image of my germ plates being fluffy I checked the T1s I took from them and they are looking pretty normal all ready. Definitely not rhizo but not super fluffy. Looks like a super wide leading edge. Like a brim on a build plate.


I may take transfers from this one today. It's fucking snowing again here for some reason so probably not painting today.


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Randalf the Grey
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Re: Swabbing a print [Re: A.k.a]
    #26534725 - 03/14/20 12:29 PM (3 years, 10 months ago)

So I can take that little fluffy piece already and inoculate the grain? It’s like a small ball of fuzzy mycelium. It doesn’t have to be more and grown out? It’s already ready for grain?


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Edited by psycho_nauticus (03/14/20 12:37 PM)


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