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A.k.a
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Re: Swabbing a print [Re: Crackatoa]
#26526086 - 03/09/20 08:08 PM (3 years, 10 months ago) |
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I used the same print for lagm and had growth within a week.
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LAGM2020     
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26526093 - 03/09/20 08:11 PM (3 years, 10 months ago) |
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Oh wow, makes me feel like I did something wrong...Maybe I should of put more on the plate? Temperature is my room temperature, low 70's.
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psycho_nauticus
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Re: Swabbing a print [Re: woofwoof]
#26526099 - 03/09/20 08:16 PM (3 years, 10 months ago) |
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Believe me, I have been...
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woofwoof
such mushrooms!



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As long as you don't see contams your good! I would suggest to be patient. I bet you there are spores on there! Just let em make love and germinate
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A.k.a
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Re: Swabbing a print [Re: woofwoof]
#26526252 - 03/09/20 09:56 PM (3 years, 10 months ago) |
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You could always restreak them after a week or whatever. Based on my very limited experience with spore plates if they’re gonna germinate it’ll be happening by a week. I’d say something like 95% of my plates either germinated in a week or still had no growth at a month.
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Cootermonkey
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Re: Swabbing a print [Re: A.k.a]
#26526623 - 03/10/20 04:59 AM (3 years, 10 months ago) |
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Heres a little confirmation that you got more than enough spores on it im sure. Its purty cool how many are actually there haha https://www.shroomery.org/forums/showflat.php/Number/21365919#21365919
And all it takes is 2 to come together
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Edited by Cootermonkey (03/10/20 05:02 AM)
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26527131 - 03/10/20 11:40 AM (3 years, 10 months ago) |
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AKA we have germination!!! I can see visible germination in only one spot of my streak. That also happens to be the very first point the cutip touched when I started the streak which has the most spores. When would be the point to transfer from a germination?
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A.k.a
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Yeeeeeaaahhh boooyyyeee!!
Lol I usually see the first growth in that spot too, or if I do zig zags a lot of times the corners go first.
Give it a day or two and see how it looks, maybe take a tiny tiny transfer.
How many days was it? I went and looked through my lagm journal and it doesn’t say exactly but it was either day 3 or 4 for me.
Ahh such a relief right.
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Edited by A.k.a (03/10/20 11:50 AM)
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26527166 - 03/10/20 12:01 PM (3 years, 10 months ago) |
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It was exactly one week. I did a zig zag streak also with the cutip, but I should of swirled the cutip around as I did the streak because there was still a good amount on the sides of the cutip. That's the strongest deposit of spores at the very beginning and since I just used the tip of the cutip, it spread thin, looking like nothing. I have some other black spots at the corners of the zig zag, VERY faint, nowhere how it looked when the cutip first touch. I wonder if those other faint black spots will germinate also, but I'm not holding my breath for it because this should be good enough right? So when I take a transfer am I supposed to avoid the actual spores themselves? That means it would have to grow out pretty wide for me to get a transfer?
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A.k.a
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Don’t worry about spores when you transfer, they’re all over but I think usually by the time you’re taking transfers they’ll have grown if they’re gonna.
A bunch of my plates ended up getting growth along the entire swipe pattern, but usually it starts at the very first contact spot and the corners.
I did swipe a print recently to two plates and only had germination in one spot which is lame. But better than nothing!
If you get more they seem usually grow in little contained colonies that wall themselves off when they hit each other like this. This is about when I took transfers, the growth spots are big enough to see good spots and get clean wedges.

But with aa+ it was just a giant thick tangle of myc. Might actually be better if you have less germination in that aspect.
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Edited by A.k.a (03/10/20 12:21 PM)
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26527216 - 03/10/20 12:33 PM (3 years, 10 months ago) |
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Well hopefully I get a couple more spots of germination...
Hopefully my one spot germinates that well!
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Crackatoa
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You will
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26530833 - 03/12/20 11:39 AM (3 years, 10 months ago) |
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So what should I be transferring from this germination plate of AA? It’s just a big ball of white over the black spores, and other spots of the streak are starting to germinate also. Do I cut a piece of mycelium that still has black spores in it from the streak? Or do I need to wait for clear margins as usual and only take mycelium?
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Crackatoa
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Picture?
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26534041 - 03/14/20 06:56 AM (3 years, 10 months ago) |
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Dude you weren't joking about AA+ being a tangled web of mycelium. I took two transfers once they germinated, and they're like two big poof balls of whiteness. It's starting to grow out a little on the agar, but about how long or how many transfers until good growth? Or is it always like this? Should it spread and colonize the agar faster since I used your agar recipe? (Remember I did all the rest of my plates 7 grams agar/10 LME, does that affect it somehow?)
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Randalf the Grey
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I never played with AA+ on agar enough to really isolate anything but yeah it has ways looked super white and puffy for me. Everytime I grew them out they just looked different than my other cubes. These are two germ plates for my current restart. Old dirty prints so still need cleaning but definitely has that puffy growths
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A.k.a
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Mine straightened up on t3. I’ve got a t5 now that’s just thin flat rhizo.
But yeah it was totally different from all the other cubes, my germ plate I thought it was gonna hit the lid.
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26534198 - 03/14/20 08:40 AM (3 years, 10 months ago) |
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Yeah I'm wondering when it's going to stop poofing up in a big ball and I can get some flat growth on agar to put to grain. I haven't been able to even put any agar to grain yet! Is it just going to keep doing that and not spread and colonize the agar? Or just keep taking transfers from the edge and I'll get some flat rhizo growth?
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A.k.a
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It’ll spread.
You can definitely put the crazy fluffy growth to grain no problem as long as it’s clean.
The more transfers/isolation you do the more uniform it should get. But also less diversity.
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Randalf the Grey
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Re: Swabbing a print [Re: A.k.a]
#26534320 - 03/14/20 09:38 AM (3 years, 10 months ago) |
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It's funny I caught this thread just now. I put fluffy stuff to grain more than once and as A.k.a. (I think it was, didn't look at name) said as long as it's clean your good. However, right after I post image of my germ plates being fluffy I checked the T1s I took from them and they are looking pretty normal all ready. Definitely not rhizo but not super fluffy. Looks like a super wide leading edge. Like a brim on a build plate. 
I may take transfers from this one today. It's fucking snowing again here for some reason so probably not painting today.
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