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psycho_nauticus
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Swabbing a print
#26509852 - 02/29/20 02:33 PM (3 years, 10 months ago) |
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I have a bunch of sterile curios, and I want to inoculate agar via maybe T streak or may just zig zagging some lines. Do I literally just take dry my cutip and roll it over the print on the foil, and then directly streak the agar? Or do I need to wet the cutip or anything? I was just going to do it dry, rub it against the foil, and then streak the agar.
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Kmacmo
The aborted pin



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Dry is fine as long as its sterile the spores will still stick to it nicely. If it's a dark print just pick a spot jab the qtip on the foil and twist then pull back you should see plenty of spores on the end of it.
Wet is fine also as long as the waters sterile, like a spore Syringe just squirt few drops onto qtip and then inoculate plate.
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Crackatoa
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Are the swabs sterile? if using Q tips you need to PC first. If they are sterile open package, roll swab in print and swipe away
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psycho_nauticus
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Re: Swabbing a print [Re: Crackatoa] 1
#26509869 - 02/29/20 02:55 PM (3 years, 10 months ago) |
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Yes they are sterile. I have everything sterile still in the package. So I I can do it all dry? Just rub and circle the cutip in foil and then streak plate? Should I expect it to feel really stick then on the agar?
Do I need to rub it on the agar first to get it sticky and then roll the cutip in the foil? Or I can just go straight to foil dry?
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Crackatoa
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Straight to foil dry, swirl Q tip to get some on it. Don't waste the whole print, it only takes a little. But you should see some spores on the agar. It doesn't take a lot of pressure to destroy the agar depending on hardness of plate. the plate will feel smooth to the q tip as well. I swirl the q tip as I swipe, Sometimes I zip zag, sometimes I just lines, sometimes I put dots.
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A.k.a
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Re: Swabbing a print [Re: Crackatoa]
#26509910 - 02/29/20 03:39 PM (3 years, 10 months ago) |
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Yeah I definitely split the agar my first time.
Use just enough pressure to keep the swab on the surface while you drag it.
Which print(s) are you going with?
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LAGM2020     
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Yeah, just swipe with a dry sterile q-tip and yer good to go
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psycho_nauticus
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Re: Swabbing a print [Re: Crackatoa]
#26510019 - 02/29/20 04:49 PM (3 years, 10 months ago) |
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Yeah they're sterile. Do you recommend any type of streak technique? Or just zig zag?
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26510022 - 02/29/20 04:51 PM (3 years, 10 months ago) |
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Albino A+ is calling my name. They look so bad ass and trippy and look fucking cool. I would love to have an experience with those
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Id recommend a simple straight-line streak but that's up to your personal preference.
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psycho_nauticus
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I did a zig zag streak, this was about 3 days ago and no germination. Now I'm concerned if I didn't get enough spores from the print or not. I covered the tip of the cutip from the print, and when I streaked there was only some black in the first and second lines. The other lines you can see marked in on the agar, but no spores. But I'm assuming they're there, just microscopic... I thought I would of seen some type of germination by now, I thought prints germinate quicker than a drop of spore syringe solution.
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Crackatoa
Stranger in a strange land



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Spores on a print are dry, they will rehydrate on the agar. print to agar can take up to 2-3 weeks sometimes. Spore syringe will generally go a little quicker.
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psycho_nauticus
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Re: Swabbing a print [Re: Crackatoa]
#26523833 - 03/08/20 12:02 PM (3 years, 10 months ago) |
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Oh I thought it was the other way around.
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Sockadin



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What type of swabs are you using? Amazon and you can get medical grade EEOG sterlized cotton swabs cheep. I use them for PE varieties.
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psycho_nauticus
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Re: Swabbing a print [Re: Sockadin]
#26524071 - 03/08/20 02:50 PM (3 years, 10 months ago) |
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Medical sterile. So why do you think? Normal to take longer? I’m thinking that I should have gotten more spores in my swab maybe...?
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A.k.a
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That’s the worst part about spore plates. You never know wtf is going on and feel like maybe somehow none of those millions of spores made it to the agar lol.
How many plates did you swipe?? 3 days would be like the absolute earliest unless they’re straight off the cap fresh.
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26524184 - 03/08/20 03:57 PM (3 years, 10 months ago) |
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I swiped one plate.
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psycho_nauticus
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Re: Swabbing a print [Re: A.k.a]
#26526024 - 03/09/20 07:23 PM (3 years, 10 months ago) |
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I'm trying to keep myself busy with getting other supplies and thinking of other things to get down the road while I'm still waiting for this AA+ that I swiped. I don't know what to do in the meantime it's killing me!!! Should I of swiped more than one plate of AA+ and swiped a couple plates of AA+? Or do you think this is normal for time? I see some spores that I got on there from streaking, but not every line is black. I have two faded black lines, and the other lines you can't see anything, just the indentation of my swipe...
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woofwoof
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I swabbed some rusty whytes from spores to agar and it took about 4 days to see any growth.
If you have any spores left over, make a syringe, make agar, do somehting mycology related to keep your mind off of it. I know that feeling!
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Crackatoa
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Sometimes you don't always see them, they're there. I cant say that I wouldn't have swiped 2-3 but I do singles more often than not. How fresh is the print?
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