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OfflinePewpewlazers
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Registered: 02/25/20
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New cat in Town
    #26507893 - 02/28/20 09:58 AM (3 years, 10 months ago)

So my buddies cat has decided to give BRF cakes a whirl. Did the usual 2:1:1 ratio, pc at 15psi for 90mins, and inoculated earlier today in a SAB. 10 cakes. 5 LGT and 5 B+.He ran into a purrdicerment after finishing inoculations. DJ meow mix initially flame stelerilzed,  70%iso wiped the needles,  and syringe ports then went to work per jar. Wiping down injection sites and needle per injection, moving to each jar until 5ccs of said genetics syringe was used. Later reading people flame sterilize in between each jar. (which sounded familiar, was read before, and in the moment forgot the step.) Moving forward DJ MM will be flame sterilizing between jar, but was wondering how critical of a mistake this is/was. He also has some rye ready to boil and PC with some qt jars with SHIP that will be going on later, and is looking for pointers while working in the SAB. Thanks -Dj Meow Mix


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OfflineShroomhunts
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Re: New cat in Town [Re: Pewpewlazers]
    #26507934 - 02/28/20 10:25 AM (3 years, 10 months ago)

I never do flame sterilization seems like overkill


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Invisiblefilthyknees
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Re: New cat in Town [Re: Shroomhunts]
    #26508024 - 02/28/20 11:29 AM (3 years, 10 months ago)

You  dont wipe with iso after flaming, that defeats the purpose.

Have you watched "let's grow mushrooms" series ? It has a sterile tek section, check YouTube.

And welcome to shroomery.


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But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineSockadin
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Re: New cat in Town [Re: filthyknees]
    #26508037 - 02/28/20 11:38 AM (3 years, 10 months ago)

Your more likely to see a contamination from the MS syringe than the lack of wiping the needle or flame sterilizing it.


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OfflinePewpewlazers
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Re: New cat in Town [Re: filthyknees]
    #26508048 - 02/28/20 11:45 AM (3 years, 10 months ago)

I'm using a lighter, and read wiping the soot off was ideal if flame sterilizing with such source? I believe I have watched it, I'll give it another check as well. Thanks for the responses guys! & hope for some decent results moving forward.


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OfflineShroomhunts
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Re: New cat in Town [Re: Pewpewlazers]
    #26508058 - 02/28/20 11:50 AM (3 years, 10 months ago)

If you wanna be extra with it a still air box is much more beneficial than flame sterilizing


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OfflinecronicrFacebook
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Re: New cat in Town [Re: Shroomhunts]
    #26508070 - 02/28/20 11:56 AM (3 years, 10 months ago)

the spot is fine it's sterile. flaming is proper technique and anybody telling you otherwise says you should avoid their advise. wiping will sanitize the outside of the needle, flaming sterilized inside and out


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I'm tired do me a favor


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OfflineShroomhunts
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Re: New cat in Town [Re: cronicr]
    #26508078 - 02/28/20 11:57 AM (3 years, 10 months ago)

If using sterile needles why would you need to flame it?


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OfflinecronicrFacebook
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Re: New cat in Town [Re: Shroomhunts]
    #26508085 - 02/28/20 12:01 PM (3 years, 10 months ago)

it's only sterile on your first jar/ hole.
If one contaminates your simply dragging it down the line of jars.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineShroomhunts
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Re: New cat in Town [Re: cronicr]
    #26508089 - 02/28/20 12:04 PM (3 years, 10 months ago)

Ahh I see thanks for clearing that up, never had a problem with it tho


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Offlinetrubblesome
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Re: New cat in Town [Re: Shroomhunts] * 1
    #26508115 - 02/28/20 12:22 PM (3 years, 10 months ago)

probably gonna be hard for a cat to do all that without opposable thumbs. good luck to him.


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