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OfflineEclipse3130
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Huge contamination outbreak - Flowhood question
    #26507148 - 02/27/20 07:54 PM (3 years, 10 months ago)

To make a 4 month story short.. scroll down for TLDR

Basically all of my agar plates are infected with a white pinhead type mold, that follows the mycelium as it colonizes..

I initially contracted the mold from using a spent, dirty flowhood that did not filter very well anymore, and from there transfered it to everything else(some without knowing)

I recently got a brand new flow hood, and started doing transfers from old(previously used)agar plates in the fridge that looked good. The mold never manifested on these plates because they were refrigerated, and probably because colonized myc has a harder time contracting things, but that doesn't mean the spores weren't still on them.

I eventually spread it to every single one of my jars and plates and just recently noticed.

TLDR
My question is can a brand new flow hood be contaminated with mold spores as well? From a dirty environment? Meaning it is pumping them out rather than filtering a sterile flow? Part of me knows that doesn't really make sense, as the job of the flowhood is to produce a sterile flow, and everyone else's flowhood wouldn't work in that case because contaminants are always floating in the air.

My plates do not receive this mold anywhere else on the plate other than where it directly follows the mycelium.

Here is a picture of the contaminated plates, i have about 50 plates that host this same problem.

Due to the fact the contamination is not present anywhere else, does this conclude it is not my filter, but rather an obvious error on my part, coming from dirty transfers



--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


Edited by Eclipse3130 (02/27/20 08:02 PM)


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OfflineLogicaL ChaosM
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26507155 - 02/27/20 08:01 PM (3 years, 10 months ago)

White pinhead mold?

Damn, thats like an infection from shroomy Hell. No idea about dirty flowhoods but this has me curious to say the least....


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: LogicaL Chaos]
    #26507166 - 02/27/20 08:10 PM (3 years, 10 months ago)

Quote:

LogicaL Chaos said:
White pinhead mold?

Damn, thats like an infection from shroomy Hell. No idea about dirty flowhoods but this has me curious to say the least....




Most likely white pinhead mold, or something of that nature. It's been plaguing me for 4 months straight and it feels like I've been thrown into an advanced level at a beginner stage 🤣


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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OfflineLogicaL ChaosM
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26507168 - 02/27/20 08:12 PM (3 years, 10 months ago)

Damn. Thats crazy.

Are u growing different varieties? Have u been able to produce any fruits in those 4 months?


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: LogicaL Chaos] * 1
    #26507171 - 02/27/20 08:15 PM (3 years, 10 months ago)

Quote:

LogicaL Chaos said:
Damn. Thats crazy.

Are u growing different varieties? Have u been able to produce any fruits in those 4 months?




About 15 different strains, but I somehow contaminated every one without knowing it with this mold until now.

I have not had a single jar of clean spawn let alone any fruits. And i'm still not giving up! :lol:

Now that I know the issue makes a world of difference, I couldn't notice it in the plates initially as I always viewed them from above or upside down and never at an angle to see vertical mold growth piggybacked on the myc. I assumed the rest of the plate was clean, my cultures were clean.


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130] * 1
    #26507172 - 02/27/20 08:15 PM (3 years, 10 months ago)

throw some blank plates in front of it and see what happens. clean any suspect plates in a sab where it's not blowing spores everywhere


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: cronicr]
    #26507180 - 02/27/20 08:21 PM (3 years, 10 months ago)

Quote:

cronicr said:
throw some blank plates in front of it and see what happens. clean any suspect plates in a sab where it's not blowing spores everywhere




I'll get on that, but part of me knows nothing will grow on it, wouldn't this mold be elsewhere on my plates rather than only piggybacking on the mycelium itself? I will still do the tests to be sure


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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OfflineLogicaL ChaosM
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26507188 - 02/27/20 08:24 PM (3 years, 10 months ago)

Perhaps the white pin mold needs mycelium as a host :strokebeard:

Do u have a SAB to use as a control?


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: LogicaL Chaos]
    #26507195 - 02/27/20 08:28 PM (3 years, 10 months ago)

Yes, I also don't believe any of these plates can really be saved. They are smothered in it. Lol I'd just be transferring more mold


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130] * 1
    #26507464 - 02/28/20 12:18 AM (3 years, 10 months ago)

Here's another picture of a different strain, it's that vertical growing mold piggybacking on the mycelium. Somehow it managed to make it's way onto all 15 of my strains starting from a clean agar plate

I believe I infected my original plates transferring using my old flow hood, somehow every transfer has this same exact mold. I'm starting from a couple fresh clean plates that haven't been touched yet and hoping for the best. I suspect I won't develop this mold as it's not seen anywhere else on the plate besides on the mycelium itself(if I was introducing it to bad sterile technique it would appear in other spots, and most likely a variety other than this singular outbreak) instead I have literally just been planting mold from place to place, my original plates don't show signs because they are refrigerated and fully colonized, no fluffy growth.





--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


Edited by Eclipse3130 (02/28/20 12:27 AM)


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Invisiblec10h12n2o
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130] * 1
    #26507488 - 02/28/20 12:30 AM (3 years, 10 months ago)

I think your plates have coronavirus bro

Someone coofed on them


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Re: Huge contamination outbreak - Flowhood question [Re: c10h12n2o]
    #26507493 - 02/28/20 12:35 AM (3 years, 10 months ago)

Quote:

c10h12n2o said:
I think your plates have coronavirus bro

Someone coofed on them



Damn pesky Corona virus.


--------------------
Trade List
🖕🖕🖕
6 hole Mini Monos


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: c10h12n2o]
    #26507497 - 02/28/20 12:38 AM (3 years, 10 months ago)

I'm secretly the breeder of the corona virus, this is my new white phenotype about to unleash it in a mall. But seriously, I'm trashing every plate and jar and starting from fresh material, if it still develops, I'll be bamboozled.


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26507561 - 02/28/20 02:46 AM (3 years, 10 months ago)



--------------------
Everything i say is completely hypothetical...



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Re: Huge contamination outbreak - Flowhood question [Re: Hobbyist]
    #26507568 - 02/28/20 03:05 AM (3 years, 10 months ago)

I had a similar problem. O burned it all to the ground started over with new spores and an SAB. Sometimes shits too fucked to bother with. Unless you have some great culture that you have been working on for years then....


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OfflineLogicaL ChaosM
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26507645 - 02/28/20 05:54 AM (3 years, 10 months ago)

I have to say, this is one of the strangest, most sneaky infectious mold Ive ever seen.

Usually molds are super obvious to find. But not this one. Its like this mold evolved to become fully symbiotic with shroom mycelium.

Its gotta be some kind of record....


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: LogicaL Chaos]
    #26508105 - 02/28/20 12:13 PM (3 years, 10 months ago)

Quote:

LogicaL Chaos said:
I have to say, this is one of the strangest, most sneaky infectious mold Ive ever seen.

Usually molds are super obvious to find. But not this one. Its like this mold evolved to become fully symbiotic with shroom mycelium.

Its gotta be some kind of record....




It does seem to ride right on top of it and no where else. I have some original clean plates that probably have the spores on them, but I'm gonna work with them and isolate the good sections away(hopefully)


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26511917 - 03/01/20 07:58 PM (3 years, 10 months ago)

Update! NO CONTAMS!

this fuzzy growth happens when your nutrient content is TOO HIGH in your agar mixture.

My recipie was too high in nutrients 27% agar 35% malt 35% dextrose


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26511921 - 03/01/20 08:00 PM (3 years, 10 months ago)

:awesomenod:


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Re: Huge contamination outbreak - Flowhood question [Re: InfiniteDreams]
    #26511937 - 03/01/20 08:12 PM (3 years, 10 months ago)

This whole time I'm like.... I don't see anything. Have to put it to grain? Nice that you realized it was just fuzzy mycelium.


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: Sockadin]
    #26511943 - 03/01/20 08:16 PM (3 years, 10 months ago)

Quote:

Sockadin said:
This whole time I'm like.... I don't see anything. Have to put it to grain? Nice that you realized it was just fuzzy mycelium.




The mycelium is naturally rhizomorph on most of my strains, I was using a different agar mixture that I didn't realize had a ton of dextrose added to it as it was a pre-made mix.

All mycelium will look like this on plates if you have a high enough nutrient content, I believe


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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Invisiblec10h12n2o
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26512075 - 03/01/20 10:59 PM (3 years, 10 months ago)

I still think its coronavirus


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: c10h12n2o] * 1
    #26539791 - 03/17/20 01:02 AM (3 years, 10 months ago)

Some updated pictures still trying to figure this out.

I'm still using the heavy nutrient agar which I guess has been known to benefit contams, and also fluffy Mycelium growth.. Just made a few more plates with 1:1 ratio agar to LME, hopefully will tell the tale of what's going on

Confusing to tell if these plates are healthy or not, all my plates have some degree of vertical fluffy growth when viewed from the side, some more tomentose strains show it better

I have made quite a few test plates in front of my flow hood and nothing has grown on them in weeks, so I know the hood isn't the problem. If it is a contam it is coming from bad technique or I'm directly transferring it from my original clean plates, which would make the most sense as it's not popping up anywhere else on the plate







--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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InvisibleHobbyist
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130] * 1
    #26540094 - 03/17/20 06:58 AM (3 years, 10 months ago)

I dunno brother, to me those look healthy. 

I'm surprised at the heavy riso growth on high nutrition plates.


--------------------
Everything i say is completely hypothetical...



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OfflineLogicaL ChaosM
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26540099 - 03/17/20 07:01 AM (3 years, 10 months ago)

Those plates look clean to me :shrug:


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Re: Huge contamination outbreak - Flowhood question [Re: LogicaL Chaos]
    #26540384 - 03/17/20 09:32 AM (3 years, 10 months ago)

I think he switched plates 15 days ago and these are low nute plates.


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Offlinemigraineur
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Re: Huge contamination outbreak - Flowhood question [Re: Eclipse3130]
    #26540418 - 03/17/20 09:53 AM (3 years, 10 months ago)

By the way, you can make your expensive HEPA filter last a lot longer by jerry rigging a cheaper pre-filter on your air intake. Furnace filters work really well. You can cut them up and use duct tape to keep them in place.


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Re: Huge contamination outbreak - Flowhood question [Re: migraineur]
    #26541002 - 03/17/20 03:10 PM (3 years, 10 months ago)

I don't because if your in a relatively clean environment like a extra bathroom or closwt I saw someone post that their  efficiency improves over time. And if it is on the internet, it's got to be true.


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Re: Huge contamination outbreak - Flowhood question [Re: Sockadin]
    #26541036 - 03/17/20 03:31 PM (3 years, 10 months ago)

I have exactly the same growth on my plates as you, but for me it's clearly LME and/or low nutes-> tomentose, fluffy, and grain water/high nutes->rhizo. When I lower the nutes in the grain water by diluting it, I can observe how the myc gradually becomes more tomentose.

Grain water/high nutes


LME 2%, low nutes


How much MEA/Dextrose do you use?


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OfflineEclipse3130
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Re: Huge contamination outbreak - Flowhood question [Re: sporecap]
    #26541066 - 03/17/20 03:51 PM (3 years, 10 months ago)

The ratio is excessive on the pictured plates, 30% dextrose 30% LME. It makes me wonder if I'm growing bacteria because of the high nutrient content and then transferring it to my oat jars. I just made some new ones with 8g LME to 10g agar ratio to see if anything changes

I'm also new to using oats and don't know how to read them, so that's overall why I'm so concerned.

A couple 5 days into G2G, other jars are all agar to grain





No flash


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


Extras: Filter Print Post Top
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