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OfflineZombie-ant
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Agar Petri dishes first time attempts
    #26506908 - 02/27/20 05:30 PM (3 years, 10 months ago)

Wondering if any body will to tell me off, advise or enlighten me or all of the above, based on the following pics?
Numbered 1 - 6



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Offlineantmanmax
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26506925 - 02/27/20 05:38 PM (3 years, 10 months ago)

Make new plates. Don't put any spores in them. If they contaminate, your sterile technique sucks. If they don't, the syringe sucks.

You might be able to do a transfer from 5, but honestly I'd just start over. What tek are you following?


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Offlineoldest hippie
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26506941 - 02/27/20 05:45 PM (3 years, 10 months ago)

All of them seem to be contaminated. There are some white fuzzy spots on a couple of the plates that
may be of interest. The pictures are not clear enough to make a determination. Let them grow
out for a couple of days or a week and see if the white spots grow large enough to identify.
For sure if there is mycelium present, it will take some work to get a clean plate.


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Offlineshevanel
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Re: Agar Petri dishes first time attempts [Re: oldest hippie]
    #26506961 - 02/27/20 05:57 PM (3 years, 10 months ago)

ew what are you growing lol


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OfflineFrankRhizo
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Re: Agar Petri dishes first time attempts [Re: shevanel]
    #26507117 - 02/27/20 07:31 PM (3 years, 10 months ago)

Bad bacterial infection. You could carry on with those but I would definitely start new plates.


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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26507616 - 02/28/20 05:07 AM (3 years, 10 months ago)

Did you streak them with a loop?


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: Neowynd8]
    #26507652 - 02/28/20 05:59 AM (3 years, 10 months ago)

Quote:

Neowynd8 said:
Did you streak them with a loop?



Yep


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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26507777 - 02/28/20 08:13 AM (3 years, 10 months ago)

There is plenty of growth in there that you can transfer. For some reason you are getting alot of bacteria either dirty innoculant or you aren't sterilizing loop good enough. Or maybe you wiped with alcohol afterwards. Idk.

The sattelite colony of mold on the edge of plate 1 and 2 is from bad sterile Tek most likely. Probably passed your hand over the open plate.

Toss plate number 4 immediately I see nothing useful there all bacteria. Go ahead and toss 1 as well there is clean mycelium there but a little too close to contams for comfort.

2, 3, 5 look like good candidates to transfer from. Avoid that yellow slimey looking shit at all costs. That's bacteria.

I'd grab one of the colonies on plate 2 asap before the mold gets any worse it's already started sporulating.

Then take additional couple transfers from 3 and 5. There is also a colony on plate 6 at 6 o'clock that can be saved. Right above the bacteria.

No need to toss ALL these plates this is what agar is for, separating clean mushroom mycelium from contaminates.

Goodluck. You got this. :thumbup:


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: gizmo1]
    #26509147 - 02/29/20 02:29 AM (3 years, 10 months ago)

Quote:

gizmo1 said:
There is plenty of growth in there that you can transfer. For some reason you are getting alot of bacteria either dirty innoculant or you aren't sterilizing loop good enough. Or maybe you wiped with alcohol afterwards. Idk.

The sattelite colony of mold on the edge of plate 1 and 2 is from bad sterile Tek most likely. Probably passed your hand over the open plate.



Probably.... need to practice

Quote:


Toss plate number 4 immediately I see nothing useful there all bacteria. Go ahead and toss 1 as well there is clean mycelium there but a little too close to contams for comfort.



Will do today.

Quote:



2, 3, 5 look like good candidates to transfer from. Avoid that yellow slimey looking shit at all costs. That's bacteria.



πŸ‘πŸΌ

Quote:


I'd grab one of the colonies on plate 2 asap before the mold gets any worse it's already started sporulating.



βœ…

Quote:



Then take additional couple transfers from 3 and 5. There is also a colony on plate 6 at 6 o'clock that can be saved. Right above the bacteria.




Quote:



No need to toss ALL these plates this is what agar is for, separating clean mushroom mycelium from contaminates.



That’s what I thought too

Quote:



Goodluck. You got this. :thumbup:




Cheers, let’s see what I can salvage.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26509167 - 02/29/20 03:26 AM (3 years, 10 months ago)

The pictures below are what I intend to cut out and place on other dishes....
Yay or nay?


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26509193 - 02/29/20 04:26 AM (3 years, 10 months ago)

I’ve probably done this wrong... but I learn best from mistakes.
I have cut and transferred to 4 other dishes as below


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Edited by Zombie-ant (02/29/20 04:33 AM)


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Offlineantmanmax
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26509235 - 02/29/20 05:15 AM (3 years, 10 months ago)

Quote:

Zombie-ant said:
I’ve probably done this wrong... but I learn best from mistakes.
I have cut and transferred to 4 other dishes as below




What agar tek are you following? Usually people take much smaller cuts of their agar. The idea is you want to clean up the plate, not move the contams from one plate to another. The smaller the section you cut out, the less likely you'll transfer contams.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: antmanmax]
    #26509257 - 02/29/20 05:43 AM (3 years, 10 months ago)

Quote:

antmanmax said:
Quote:

Zombie-ant said:
I’ve probably done this wrong... but I learn best from mistakes.
I have cut and transferred to 4 other dishes as below




What agar tek are you following? Usually people take much smaller cuts of their agar. The idea is you want to clean up the plate, not move the contams from one plate to another. The smaller the section you cut out, the less likely you'll transfer contams.



The agar tek I’m using is the Zombie-Ant (doesn’t know what the fuck he is going) tek.

Let’s see what happens


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Offlineantmanmax
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26509747 - 02/29/20 12:55 PM (3 years, 10 months ago)

Quote:

Zombie-ant said:
Quote:

antmanmax said:
Quote:

Zombie-ant said:
I’ve probably done this wrong... but I learn best from mistakes.
I have cut and transferred to 4 other dishes as below




What agar tek are you following? Usually people take much smaller cuts of their agar. The idea is you want to clean up the plate, not move the contams from one plate to another. The smaller the section you cut out, the less likely you'll transfer contams.



The agar tek I’m using is the Zombie-Ant (doesn’t know what the fuck he is going) tek.

Let’s see what happens




Lol. Zombie Ant might learn best from reading some of the many excellent Agar teks, where people have made your mistakes for you!


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OfflineA.k.aM
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Re: Agar Petri dishes first time attempts [Re: antmanmax]
    #26509760 - 02/29/20 01:07 PM (3 years, 10 months ago)

Yeah transfers are usually as small as possible, taking the whole thing was pointless it didn’t isolate good growth.

What are you trying to achieve exactly?


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: A.k.a]
    #26509840 - 02/29/20 02:20 PM (3 years, 10 months ago)

Quote:

A.k.a said:
Yeah transfers are usually as small as possible, taking the whole thing was pointless it didn’t isolate good growth.

What are you trying to achieve exactly?



To isolate good growth πŸ˜‚
I’ll transfer again, but a Smaller amount, I have more plates


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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26510360 - 02/29/20 08:20 PM (3 years, 10 months ago)

Hahahahaha.
Bro.
I don't even know what to say.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: gizmo1]
    #26510595 - 02/29/20 11:39 PM (3 years, 10 months ago)

Quote:

gizmo1 said:
Hahahahaha.
Bro.
I don't even know what to say.




You don’t need to say anything, just laugh and be merry πŸ€·πŸ»β€β™‚οΈ


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26512884 - 03/02/20 01:34 PM (3 years, 10 months ago)

Sooo

Yes I know there is condensation, I’m still learning

What’s happening with these, there is growth, but is it the wrong kind.
Shall I transfer again, but this time smaller?



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Invisiblealaskappalachian
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26512925 - 03/02/20 01:59 PM (3 years, 10 months ago)

Oof.  I'd let that creep out onto the new plate a bit and see what emerges.  Looks like you have some growth to pull from (on pic 3 for instance, you might wwant to grab the leading edge of that emerging growth between 8-9o'clock).


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: alaskappalachian]
    #26512952 - 03/02/20 02:17 PM (3 years, 10 months ago)

Quote:

alaskappalachian said:
Oof.  I'd let that creep out onto the new plate a bit and see what emerges.  Looks like you have some growth to pull from (on pic 3 for instance, you might wwant to grab the leading edge of that emerging growth between 8-9o'clock).



Sorry to sound like an idiot what do you mean by β€œgrad the leading edge”


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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26513143 - 03/02/20 04:11 PM (3 years, 10 months ago)

Transfer from 8:00 I think was what he ment.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: Sockadin]
    #26513765 - 03/02/20 11:14 PM (3 years, 10 months ago)

Quote:

Sockadin said:
Transfer from 8:00 I think was what he ment.



Like, how much: 5mm2 ?


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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26514191 - 03/03/20 08:35 AM (3 years, 10 months ago)

The size of a grain of rice.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: InfiniteDreams]
    #26514831 - 03/03/20 03:30 PM (3 years, 10 months ago)

Quote:

InfiniteDreams said:
The size of a grain of rice.



Ok that’s small πŸ‘πŸΌπŸ€™πŸΌ


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26514840 - 03/03/20 03:35 PM (3 years, 10 months ago)

I’m sure everyone is shouting at me

I haven’t transferred yet

Am I seeing rhizomorphic growth?



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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26514864 - 03/03/20 03:48 PM (3 years, 10 months ago)

Picture 3-4 looks bad. I wouldn't use anything.


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Re: Agar Petri dishes first time attempts [Re: Sockadin]
    #26516701 - 03/04/20 03:19 PM (3 years, 10 months ago)

These plates look rough. It will probably be easier to just start over again. Follow some of the already written teks. Bod's agar portal is great reading.
https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954


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Re: Agar Petri dishes first time attempts [Re: Magic Mushpoon]
    #26516941 - 03/04/20 05:37 PM (3 years, 10 months ago)

At this point even I recommend you start over. The mycellium has now overtook some of the bacterial growth and you wont be able to seperate the the 2. Atleast not easily.
Go ahead and start fresh. When you transfer tske transfers about the size of a grain of rice. Transfer before anything the growth gets close to any contams. Make sure nothing passes over open dishes and make sure any utensils you use are flamed red hot before use.


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Re: Agar Petri dishes first time attempts [Re: gizmo1]
    #26517229 - 03/04/20 07:29 PM (3 years, 10 months ago)

Almost a week a ago I told you to start new plates. I wasn't being a dick, use those as observation and practice plates. Try a dry run if you can. Don't inoculate just pour and seal and pour and transfer. See what happens, trust me this stuff takes practice and more practice. You have to learn what clean growth looks like and then get overconfident and learn again what clean growth looks like.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: FrankRhizo]
    #26517756 - 03/05/20 12:08 AM (3 years, 10 months ago)

Quote:

FrankRhizo said:
Almost a week a ago I told you to start new plates. I wasn't being a dick, use those as observation and practice plates. Try a dry run if you can. Don't inoculate just pour and seal and pour and transfer. See what happens, trust me this stuff takes practice and more practice. You have to learn what clean growth looks like and then get overconfident and learn again what clean growth looks like.



I have new plates that have been at room temp for 4 days... no sign of growth
I have plates that are poured apart from these in the pic cooling before I wrap with Saran Wrap (cling film for UK readers)
Here are pics of the days old dishes...
7 of these cooled and wrapped another 12 cooling


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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26517882 - 03/05/20 02:41 AM (3 years, 10 months ago)

No condensation, really clear good job.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: FrankRhizo]
    #26517887 - 03/05/20 02:49 AM (3 years, 10 months ago)

Quote:

FrankRhizo said:
No condensation, really clear good job.



Well one of the others has. Sterilising more agar at the mo, Will pour on petris that have just been oven sterilised. I’ve had cancellations today, so I can β€œwork” from home.


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Edited by Zombie-ant (03/05/20 02:51 AM)


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OfflineC12ShroomMan
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26518119 - 03/05/20 07:39 AM (3 years, 10 months ago)

Looks like your making more work for your self, donno why you basically transferred the whole dish into a new one...... its almost like you never read a single thread how to work with agar? cuttings should be from the edge of a single good looking growth site.... a bit too blurry for me to tell whats mold/myc


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