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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: alaskappalachian]
    #26512952 - 03/02/20 02:17 PM (3 years, 10 months ago)

Quote:

alaskappalachian said:
Oof.  I'd let that creep out onto the new plate a bit and see what emerges.  Looks like you have some growth to pull from (on pic 3 for instance, you might wwant to grab the leading edge of that emerging growth between 8-9o'clock).



Sorry to sound like an idiot what do you mean by “grad the leading edge”


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OfflineSockadin
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26513143 - 03/02/20 04:11 PM (3 years, 10 months ago)

Transfer from 8:00 I think was what he ment.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: Sockadin]
    #26513765 - 03/02/20 11:14 PM (3 years, 10 months ago)

Quote:

Sockadin said:
Transfer from 8:00 I think was what he ment.



Like, how much: 5mm2 ?


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InvisibleInfiniteDreams
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26514191 - 03/03/20 08:35 AM (3 years, 10 months ago)

The size of a grain of rice.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: InfiniteDreams]
    #26514831 - 03/03/20 03:30 PM (3 years, 10 months ago)

Quote:

InfiniteDreams said:
The size of a grain of rice.



Ok that’s small 👍🏼🤙🏼


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26514840 - 03/03/20 03:35 PM (3 years, 10 months ago)

I’m sure everyone is shouting at me

I haven’t transferred yet

Am I seeing rhizomorphic growth?



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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26514864 - 03/03/20 03:48 PM (3 years, 10 months ago)

Picture 3-4 looks bad. I wouldn't use anything.


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OfflineMagic Mushpoon
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Re: Agar Petri dishes first time attempts [Re: Sockadin]
    #26516701 - 03/04/20 03:19 PM (3 years, 10 months ago)

These plates look rough. It will probably be easier to just start over again. Follow some of the already written teks. Bod's agar portal is great reading.
https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954


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Re: Agar Petri dishes first time attempts [Re: Magic Mushpoon]
    #26516941 - 03/04/20 05:37 PM (3 years, 10 months ago)

At this point even I recommend you start over. The mycellium has now overtook some of the bacterial growth and you wont be able to seperate the the 2. Atleast not easily.
Go ahead and start fresh. When you transfer tske transfers about the size of a grain of rice. Transfer before anything the growth gets close to any contams. Make sure nothing passes over open dishes and make sure any utensils you use are flamed red hot before use.


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OfflineFrankRhizo
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Re: Agar Petri dishes first time attempts [Re: gizmo1]
    #26517229 - 03/04/20 07:29 PM (3 years, 10 months ago)

Almost a week a ago I told you to start new plates. I wasn't being a dick, use those as observation and practice plates. Try a dry run if you can. Don't inoculate just pour and seal and pour and transfer. See what happens, trust me this stuff takes practice and more practice. You have to learn what clean growth looks like and then get overconfident and learn again what clean growth looks like.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: FrankRhizo]
    #26517756 - 03/05/20 12:08 AM (3 years, 10 months ago)

Quote:

FrankRhizo said:
Almost a week a ago I told you to start new plates. I wasn't being a dick, use those as observation and practice plates. Try a dry run if you can. Don't inoculate just pour and seal and pour and transfer. See what happens, trust me this stuff takes practice and more practice. You have to learn what clean growth looks like and then get overconfident and learn again what clean growth looks like.



I have new plates that have been at room temp for 4 days... no sign of growth
I have plates that are poured apart from these in the pic cooling before I wrap with Saran Wrap (cling film for UK readers)
Here are pics of the days old dishes...
7 of these cooled and wrapped another 12 cooling


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OfflineFrankRhizo
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26517882 - 03/05/20 02:41 AM (3 years, 10 months ago)

No condensation, really clear good job.


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OfflineZombie-ant
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Re: Agar Petri dishes first time attempts [Re: FrankRhizo]
    #26517887 - 03/05/20 02:49 AM (3 years, 10 months ago)

Quote:

FrankRhizo said:
No condensation, really clear good job.



Well one of the others has. Sterilising more agar at the mo, Will pour on petris that have just been oven sterilised. I’ve had cancellations today, so I can “work” from home.


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OfflineC12ShroomMan
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Re: Agar Petri dishes first time attempts [Re: Zombie-ant]
    #26518119 - 03/05/20 07:39 AM (3 years, 10 months ago)

Looks like your making more work for your self, donno why you basically transferred the whole dish into a new one...... its almost like you never read a single thread how to work with agar? cuttings should be from the edge of a single good looking growth site.... a bit too blurry for me to tell whats mold/myc


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