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North Spore Shop: Cultivation Supplies

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Invisiblewoofwoof
such mushrooms!
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Registered: 01/04/19
Posts: 1,127
Noc up agar no gloves
    #26504074 - 02/25/20 09:08 PM (3 years, 10 months ago)

I was bored and decided to inoculate 5 agar dishes without the aid of gloves, bare hands 4 days ago. Used SAB. I just washed hands and used alcohol. 3 of the 5 plates have some contamination, but the other 2 have some myc growth. I made sure to not put hands over the dishes. I always wear gloves and just wanted to find out myself what would happen. Proper sanitation is key here according to this experiment


Edited by woofwoof (02/25/20 09:11 PM)


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
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Re: Noc up agar no gloves [Re: woofwoof]
    #26504110 - 02/25/20 09:28 PM (3 years, 10 months ago)

I haven't wore gloves in years most contams fall off your scalpel. we had somebody wash his hands with antibacterial soap and touch dishes and surprise surprise they didn't contaminate


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Invisiblewoofwoof
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Registered: 01/04/19
Posts: 1,127
Re: Noc up agar no gloves [Re: cronicr]
    #26504123 - 02/25/20 09:39 PM (3 years, 10 months ago)

Quote:

cronicr said:
I haven't wore gloves in years most contams fall off your scalpel. we had somebody wash his hands with antibacterial soap and touch dishes and surprise surprise they didn't contaminate



See, this is why I did this! Thanks for chiming in bud. Hand Sanitizer vs alcohol doesn't make difference then!


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Invisiblegizmo1
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Registered: 06/15/11
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Re: Noc up agar no gloves [Re: woofwoof]
    #26504179 - 02/25/20 10:47 PM (3 years, 10 months ago)

If you have good sterile Tek your hands should never pass over a open dish or jar anyways. Gloves for me are more of a me piece of mind kinda thing. Makes me feel better.


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Invisiblewoofwoof
such mushrooms!
I'm a teapot User Gallery


Registered: 01/04/19
Posts: 1,127
Re: Noc up agar no gloves [Re: gizmo1]
    #26504222 - 02/25/20 11:31 PM (3 years, 10 months ago)

Quote:

gizmo1 said:
If you have good sterile Tek your hands should never pass over a open dish or jar anyways. Gloves for me are more of a me piece of mind kinda thing. Makes me feel better.



I agree. I am just in habit to use gloves.


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InvisibleInfiniteDreams
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Registered: 10/25/19
Posts: 1,224
Re: Noc up agar no gloves [Re: woofwoof]
    #26504417 - 02/26/20 06:29 AM (3 years, 10 months ago)

I started by using gloves.  Maybe just the gloves I have but I'm already clumsy enough fumbling around in a SAB, the gloves make it a loss worse for me.  So last few times I ditched them.  Only change, still use 70% iso.  No contam issues whatsoever.


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