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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Plate pouring night thread... 1
#26503804 - 02/25/20 06:39 PM (3 years, 10 months ago) |
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So I thought, it's time to make a plate pooring thread so everyone can tell everyone else what they are pooring, pooring into, and their recipes.
Tonight I are doing 400ml of LME 7g/ agar 14g in disposable 100MM dishes. I decided to go with 20 mins at 15 PSI and I am hoping to get 50 plates if I pour it thin.
I have some transfers to make thanks to someone who hooked me up recently because all of my spores were apparently dead and old AF.
Transferring Tomorrow after a cool down some Amazon-RW-Tasmanian Cube-AA+- and a mystery spore print. All transfer plates look good so far, will update with pictures tomorrow.
Edited by Sockadin (02/25/20 07:36 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: Plate pooring night thread... [Re: Sockadin]
#26503809 - 02/25/20 06:41 PM (3 years, 10 months ago) |
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pouring
Pooring is what I go through after I pay bills.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Plate pooring night thread... [Re: mushboy]
#26503814 - 02/25/20 06:43 PM (3 years, 10 months ago) |
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Shit and now I can't fix it cause ehh. Engish 
Edit: I fixed the title, I'll leave the poor spelling up there for ya.
Edited by Sockadin (02/25/20 06:45 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: Plate pooring night thread... [Re: Sockadin]
#26503851 - 02/25/20 07:02 PM (3 years, 10 months ago) |
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Actually... I'll be pouring 500ml of brf agar tonite. Standard mushtard recipe for me!
6g brf 9g telefone agar 500ml tap water.
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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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Re: Plate pooring night thread... [Re: mushboy]
#26503890 - 02/25/20 07:29 PM (3 years, 10 months ago) |
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I've been pouring 800mL 2% agar, 1.4% LME (pilsner) Boil it, strain it through cheesecloth, PC for 20 min at 15PSI, cool to temp and pour.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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@ Mushboy, I haven't done BRF/Agar I imagine you have had great success with this recipe.
@ Infinitedreams Do you find straining it though cheesecloth helps in the clarity? My last batch I used some Pectic Enzyme to help clear the haze of that agar. It seemed to help but the growth also seems slower with that additive, I did 1G Pectic Enzyme 7G LME 12G Agar Agar.
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InfiniteDreams


Registered: 10/25/19
Posts: 1,224
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Re: Plate pooring night thread... [Re: Sockadin]
#26503957 - 02/25/20 08:02 PM (3 years, 10 months ago) |
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It solved it all for me. In my previous trials the agar looked just fine all the way through, even during the pour, and then when it would set the sediment appeared. And it wasn't evenly distributed at all. It was purely cosmetic, no issues using the agar. Based off advice from a user complaining about the same and c10 talked about it in his agar guide too. Strain through a cheesecloth. No more sediment. As for clarity, yes it extremely clear as well. I also moved from 2% to 1.4% LME at the same time, so I don't know if that played a part as well in the color/haze.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: Plate pooring night thread... [Re: Sockadin]
#26503959 - 02/25/20 08:03 PM (3 years, 10 months ago) |
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Quote:
Sockadin said: @ Mushboy, I haven't done BRF/Agar I imagine you have had great success with this recipe.
 i only really use it because i still have a fuckton of brf and brf seems to pin like fucking crazy. my lme/pda plates never pin like the brf plates.
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woofwoof
such mushrooms!



Registered: 01/04/19
Posts: 1,127
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Re: Plate pooring night thread... [Re: mushboy]
#26504003 - 02/25/20 08:28 PM (3 years, 10 months ago) |
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Quote:
mushboy said:

Actually... I'll be pouring 500ml of brf agar tonite. Standard mushtard recipe for me!
6g brf 9g telefone agar 500ml tap water.
Dude I love the simplicity of this recipe for my agar as well.
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