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OnlineEclipse3130
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Wet spot bacteria or condensation? *DELETED*
    #26502972 - 02/25/20 11:13 AM (3 years, 10 months ago)

Post deleted by Eclipse3130

Reason for deletion: Answered


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"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
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OfflineClidefrog
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Re: Wet spot bacteria or condensation? [Re: Eclipse3130]
    #26502985 - 02/25/20 11:19 AM (3 years, 10 months ago)

just a little bit of water build up. doesn't seem to be contam to me :smile:


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Offlinefeldman114
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Re: Wet spot bacteria or condensation? [Re: Clidefrog]
    #26503015 - 02/25/20 11:45 AM (3 years, 10 months ago)

Oh that’s most definitely bacterial:sad:

But you can still roll the dice in a shoebox, or case and top-fruit.


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OnlineEclipse3130
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Re: Wet spot bacteria or condensation? [Re: feldman114]
    #26503058 - 02/25/20 12:11 PM (3 years, 10 months ago)

Quote:

feldman114 said:
Oh that’s most definitely bacterial:sad:

But you can still roll the dice in a shoebox, or case and top-fruit.




Just prepping them too moist? Because I'm not introducing bacteria, rather it develops from not being dry enough I'm guessing?

I load them dry to the touch, no sticking, they develop a tad of moisture after PC but dry back out after some shakes and a few hours. They look good up until the end of colonization when they start to get wet


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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Offlinefeldman114
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Re: Wet spot bacteria or condensation? [Re: Eclipse3130]
    #26503087 - 02/25/20 12:24 PM (3 years, 10 months ago)

Moisture, on its own, can’t cause contamination. Bacteria don’t spontaneously appear.

That said, they thrive in wet conditions, so wet jars/subs are more likely to go bacterial.

The main tell-tale sign is the uncolonized grains.


What did you inoculate with? I wouldn’t be so sure your culture is clean. Many contams are invisible to the untrained eye, even on agar. This goes double for sneaky ducking bacteria dicks.


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OnlineEclipse3130
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Re: Wet spot bacteria or condensation? [Re: feldman114]
    #26503111 - 02/25/20 12:40 PM (3 years, 10 months ago)

Quote:

feldman114 said:
Moisture, on its own, can’t cause contamination. Bacteria don’t spontaneously appear.

That said, they thrive in wet conditions, so wet jars/subs are more likely to go bacterial.

The main tell-tale sign is the uncolonized grains.


What did you inoculate with? I wouldn’t be so sure your culture is clean. Many contams are invisible to the untrained eye, even on agar. This goes double for sneaky ducking bacteria dicks.




I inoculate with what appears to be a fully grown clean agar culture, but as you said it can be invisible.. so not sure how to get a clean culture if it's always invisible :lol:



--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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Offlinefeldman114
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Re: Wet spot bacteria or condensation? [Re: Eclipse3130]
    #26503261 - 02/25/20 02:04 PM (3 years, 10 months ago)

Start by isolating a culture so you have no fluff (which can be hidden contams).

I find that cloning is the easiest way to get clean growth. Even if a contam is hidden, it’s kinda obvious because growth is quick otherwise.

If I’m doubt, let a good-looking plate pin, then transfer the pin - it SHOULD be sterile (unless a mold sporulated on the plate, but that’s veeery visible).


Just an anecdote...I grew for years before getting into agar. I thought my jars were always clean until I ran a culture that colonized quicker and looked healthier than any other culture I ever worked with.
Turns out, that was my first clean spawn jar ever.

You’ll REALLY know at harvest, where clean tubs pump out waaay more than bacterial ones.


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Offlinefeldman114
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Re: Wet spot bacteria or condensation? [Re: feldman114]
    #26503267 - 02/25/20 02:08 PM (3 years, 10 months ago)



That’s the resulting tub. 4qts of spawn got me 5.5oz on first flush and over 3 on the second.
Harvesting was a biiiitch lol This pic was taken after I filled up an entire dehydrator with the shrooms from that one corner.


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OnlineEclipse3130
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Re: Wet spot bacteria or condensation? [Re: feldman114]
    #26503331 - 02/25/20 02:47 PM (3 years, 10 months ago)

I will consider that the problem, but this has even happened to me from totally clean grain using G2G transfers as well, which leads me to believe it is simply an excess moisture issue in my jars? Or introducing bacteria during transfer.

It would make more sense to me that the wet spots are not bacterial because you can have wet spots immediately following a PC cycle. And this most likely is a case of too much moisture. How can wet spots immediately after a PC cycle be bacterial?

I will find out for sure when cutting back my moisture content in my jars by drying them out further before the cycle, and changing to unmodded lids without holes.


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


Extras: Filter Print Post Top
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