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OfflineZombie-ant
Mushroom Ninja in training
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Registered: 09/02/19
Posts: 549
Loc: UK Flag
Last seen: 16 days, 15 hours
Re: Agar growth? [Re: iwh678]
    #26502652 - 02/25/20 06:44 AM (3 years, 10 months ago)

Quote:



What are you using an oven for?




Dry sterilising glass plates wrapped in foil.




Where did you open it up?



When I put it in a cooling bath...:facepalm3:


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OfflineMrBovineJoni
Glip-Glop


Registered: 11/28/19
Posts: 264
Last seen: 13 days, 16 hours
Re: Agar growth? [Re: Zombie-ant]
    #26502663 - 02/25/20 06:58 AM (3 years, 10 months ago)

Quote:

Zombie-ant said:
Quote:

iwh678 said:

Is it possible your issue is from the pour itself?



The agar was sterilised, it set then I diluted it to make make the mixture go further, I sterilised it again though after. I covered the lid, but opened it a couple of timeS while cooling, but not much 10ish secs. a couple of times while it was streaming hot. I wore a Tyvek suit, face mask, Sanitised gloves Using an SAB too.




Ideally you don't want the receiver dish petri lid to be open for longer than a second or two when working in SAB. Every second increases the chance of contamination - get spores on the sterilised loop; open the lid, swipe with the loop in the chosen pattern and carefully close the lid as soon as possible (while still doing steady motions inside SAB).


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OfflineZombie-ant
Mushroom Ninja in training
Male User Gallery


Registered: 09/02/19
Posts: 549
Loc: UK Flag
Last seen: 16 days, 15 hours
Re: Agar growth? [Re: MrBovineJoni] * 1
    #26502710 - 02/25/20 07:47 AM (3 years, 10 months ago)

Quote:

MrBovineJoni said:

Ideally you don't want the receiver dish petri lid to be open for longer than a second or two when working in SAB. Every second increases the chance of contamination - get spores on the sterilised loop; open the lid, swipe with the loop in the chosen pattern and carefully close the lid as soon as possible (while still doing steady motions inside SAB).




I will have to practice my Mushroom Ninja Skills


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