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Offlinebertblack
Feeling the cosmic energy
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Registered: 03/26/18
Posts: 191
Loc: the wopwops
Last seen: 2 years, 8 months
No Pour Agar (Holy Grails)
    #26500659 - 02/23/20 10:29 PM (3 years, 10 months ago)

Kia ora again,

Finally got a pc and can do more agar experiments now. Exciting stuff! :grin: Major shout out to Trich for showing me the way with 8oz holy grail containers for agar, they seem great for my needs so far and the clarity is great!! https://www.shroomery.org/forums/showflat.php/Number/26066566/fpart/2/vc/1#26066566



They handled the PC fine without warping or weakening the seal on the lids, had some condensation but it was easily poured out in the SAB. They were loaded into the PC in stacks of 4 wrapped in foil. The bottom 2 had almost no condensation where as the top 2 had a decent bit more. I evaporated the moisture on the lids by chucking an led aquarium lamp on top for an hour. PC'ed @ 15psi for 25 minutes, took them out while they were still hot, and cooled in the SAB.

I've been using brf agar simply because it's what I had round home. 2% agar agar, 1.5% brown rice flour, and 500ml water. Bod has a cool experiment with different strengths of brf, give it a read if you're interested. https://www.shroomery.org/forums/showflat.php/Number/23922599 :thumbup:



Melted 1/4" holes in the sides and stuffed tightttt as a moose's arse with polyfil. I fuckin love polyfil, has so many uses across all my hobbies :laugh2: I've got some really old Psilocybe Subaeruginosa spores that I'm trying to germinate too, there's just something so great about woodlovers... :wink:

I saw someone else using these deli containers for pf tek and thought that was a great idea, so versatile! Made a slurry with one of my first microwaved agar plate and poured onto a brf cake or sorts.



On a different note, it's hella cool that you can use a microwave to whip up a few plates if you're desperate, I had 2/3 success rate with my experiments so far (Got a PC now but will continue to play around with the microwave). One plate went bacterial but that was probably my error with bad technique..

This is a plate after I took a transfer, still smells fine and i'll make into a slurry and chuck it in some unsterile bulk for shits n' gigs




Peace :gethigh:


Edited by bertblack (02/24/20 02:30 AM)


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