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 920
Strangest
Registered: 01/04/20
Posts: 107
Last seen: 7 months, 14 days
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 Handling Caps While Printing 
#26499724 - 02/23/20 10:39 AM (4 years, 1 month ago) |
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Looking to print for the first time today. Reading a lot about using toothpicks and thumbtacks thru the cap for easier handling. Is this necessary? Seems like I want to avoid holding my hand over the cap if at all possible. Could I just use sterile tweezers to handle the cap by the edge or is it too heavy/floppy?
If not, could I push a toothpick thru BUT PARALLEL to the cap? So I have a handle away from the top?
Thx.
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Sockadin
 
Registered: 01/03/10
Posts: 7,248
Last seen: 1 month, 7 days
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Re: Handling Caps While Printing [Re: 920]
#26499775 - 02/23/20 11:05 AM (4 years, 1 month ago) |
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You can also get the metal kabob skewers and slide it horizontal through the cap about 1/4 from the top.
Honestly I make trash prints and just use my hands in the past. I'm curious what other people do. I need to get better and my sterile practice.
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BLINKfan420
Jedi
Registered: 07/06/12
Posts: 1,078
Last seen: 7 months, 17 days
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Re: Handling Caps While Printing [Re: Sockadin]
#26499801 - 02/23/20 11:22 AM (4 years, 1 month ago) |
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I've always used my hands and a pair of unsterile scissors off of the kitchen table. š
I just find a nice, flat, opened cap and snip it as close to the gills as possible. Then I put it on a piece of unsterile foil and place a coffee mug on top for 24-48 hours. Voila. In my opinion prints aren't really meant to be perfectly clean. Hence agar.
BLiNK
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All my pictures are drawn from imagination. I don't even know what a mushroom is.
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Sockadin
Registered: 01/03/10
Posts: 7,248
Last seen: 1 month, 7 days
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Re: Handling Caps While Printing [Re: BLINKfan420]
#26499810 - 02/23/20 11:28 AM (4 years, 1 month ago) |
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Foil comes sterile in the roll.
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LeFroid
Stranger
Registered: 10/09/19
Posts: 11
Last seen: 6 months, 21 days
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Re: Handling Caps While Printing [Re: Sockadin]
#26499843 - 02/23/20 11:57 AM (4 years, 1 month ago) |
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Wear gloves, wiped with a good amount of 70% ISO, and you should be fine. Just be careful, use a SAB, and minimize direct contact.
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 alaskappalachian
Entitiologist
Registered: 10/22/19
Posts: 1,758
Loc: The 49th Dimension
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Re: Handling Caps While Printing [Re: Sockadin]
#26499864 - 02/23/20 12:13 PM (4 years, 1 month ago) |
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Quote:
Sockadin said:
You can also get the metal kabob skewers and slide it horizontal through the cap about 1/4 from the top.
I've done this several times before. I likes this, actually... 
I've also used my hands like some kind of friggin ape before. Toothpicks work ok. You can play around and practice before you go live. I'm moving on to wire racks... Print a dozen at a time and just lift the rack. I wouldn't worry too much about it. Like Blink said: that's why we use agar. Also, my new favorite pokey tool is a large upholstery needle. The skewers aren't nearly as sharp and are fatter which can lead to flubs.
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THE 49TH MYCOJOURNAL: EXOTICS, AURORAS, & ENTITIES
ISO:Gym punctifolius spores for trade 
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Just_A_Noob
Breathing
Registered: 12/30/16
Posts: 6,809
Loc: PNW
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i wear gloves and print in the SAB
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Wearing a mask is bad for my physical, emotional, and spiritual health.
Complying = Consent
Wide Mouth 1/2 Pint No-Pour TEK
TC Teks & Links
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920
Strangest
Registered: 01/04/20
Posts: 107
Last seen: 7 months, 14 days
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Re: Handling Caps While Printing [Re: Just_A_Noob]
#26499884 - 02/23/20 12:24 PM (4 years, 1 month ago) |
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Clearly Iām overthinking the hell out of this.
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LogicaL Chaos
Ascension Energy & Alien UFOs
Registered: 05/12/07
Posts: 69,940
Loc: The Inexpressible... 
Last seen: 12 hours, 41 minutes
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Re: Handling Caps While Printing [Re: 920]
#26499897 - 02/23/20 12:40 PM (4 years, 1 month ago) |
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A large thumbtack might work pretty well. I usually just use gloved hands and pull the cap off gently or bare hands If Im feeling lazy 
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filthyknees
no coincidence
Registered: 03/08/13
Posts: 6,283
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I've taken wild spores to start agar and not had contamination so it's quiet remarkable actually how little care is needed.
So any reasonable measures to keep sterility help but yes overthinking unless just awful technique is used.
--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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