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Offline920
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Too Many Printing Teks - How's this?
    #26499069 - 02/22/20 10:49 PM (1 year, 11 days ago)

I've been reading spore printing teks all day. The more I read, the more they contradict each other. At their heart, it seems like all I want to do is get a mushroom cap to drop spores onto a sterile "carrier."

So I'm trying to consolidate all these different ideas into something that works for me.

Seems like I can just cut some 3x6" foil rectangles, place them into a makeshift foil envelope, and put them in the oven to sterilize.

Then move to a SAB box, and using the same sterile technique I use to inoculate, cut the cap off my shroom with sanitized scissors, place it on a piece of sterile foil, and loosely cover with another piece of sterile foil (similar to the "tents" in Bod's Tek). Then close off the holes in the SAB...

After X? hours, remove the foil cover, remove the cap, and fold over the printed foil. Then proceed folding the edges to seal it off and and label.

Is that it? Do I need to fuss with a "handle" (thumbtack or toothpick) for the cap?


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Offlinesonoramo
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Re: Too Many Printing Teks - How's this? [Re: 920] * 1
    #26499080 - 02/22/20 10:58 PM (1 year, 11 days ago)

IME keeping spore prints totally sterile is hopeless. Better to keep them reasonably clean by bleaching your paper, foil or other material before printing. Then, don't bother with special technique other than touch the inoculating loop only to the spores. I've always gotten some contams on germination plates. Use a SAB and sterile technique to avoid overwhelming the plates with contams, but plan for two or more transfers to clean them up.

If you really want to get sterile cultures from the get-go, better to culture from a mushroom (cutting it open with a sterile blade and then extracting a sample with a second sterile blade in the SAB) rather than from spore prints.

BTW, I like PP5 salsa or yogurt container tops for prints. After printing, I trim them, fold over, and tape the edges with ordinary Scotch tape. They are easy to clean, easy to see where the spores are and easier to handle in the SAB than paper, foil or microscope slides.

$0.02


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OfflineLogicaL ChaosM
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Re: Too Many Printing Teks - How's this? [Re: 920]
    #26499098 - 02/22/20 11:12 PM (1 year, 11 days ago)

Im currently on working on improving spore print cleanliness as well: https://www.shroomery.org/forums/showflat.php/Number/26499059

Im going to go one step further and place the foil and mushroom cap inside a half pint glass jar before opening the SAB. Im hoping this will improve cleanliness even more.

I like your needle-stab idea for the cap. Would make for a nice undisturbed print as well, for that bag appeal :lol:


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OfflineZifozonke
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Re: Too Many Printing Teks - How's this? [Re: LogicaL Chaos]
    #26499130 - 02/22/20 11:31 PM (1 year, 11 days ago)



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Re: Too Many Printing Teks - How's this? [Re: Zifozonke]
    #26499138 - 02/22/20 11:36 PM (1 year, 11 days ago)

I use small stainless steel scissors that can be sterilized with a bernzo torch. But if you elevate the cap off the foil with a cookie rack, then the caps or stem won’t touch the foil. I also have a giant pair of tweezers you can use if you make tons of prints so you are not moving your hands over the foil. If your doing a few prints you won’t need it. A cookie baking rack is a badass tool for spore printing.

I usually just use my gloved hand and only touch the top of the cap. Not underneath.


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Edited by eatyualive (02/22/20 11:43 PM)


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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