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Offlinemongoosed
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Crispr. Could this be done with mycelium?
    #26492741 - 02/19/20 01:37 AM (3 years, 11 months ago)

I have watched programs on this subject on TV and have seen proof of someone creating a button mushroom that does not brown. Achieved by editing the genes.

My question is this.

Is it possible to add the glowing jellyfish gene to mycelium?

This would be very useful for spotting contams as the mycelium would glow and the contams would not.

We would also get glow in the dark mushrooms which would be pretty cool.

https://www.the-odin.com/colorbacteria/


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InvisiblebodhisattaMDiscordReddit
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Re: Crispr. Could this be done with mycelium? [Re: mongoosed] * 1
    #26496087 - 02/20/20 09:01 PM (3 years, 11 months ago)

It's a lot easier to knock a gene out with CRIPR. And harder to put a gene in.

You need to make guide RNA for your target organism.

Kits come made to do very specific gene knockouts or insertions. They act on one specific species at one very specific area of their genome.

So yes possible but extremely expensive for now and you're going to need a genetic engineer to design your gRNA


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Offlinemongoosed
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Re: Crispr. Could this be done with mycelium? [Re: bodhisatta]
    #26496323 - 02/21/20 12:50 AM (3 years, 11 months ago)

This is an insert from the instructions on the kit i linked to.

Plasmid

A plasmid is a small piece of DNA that is connected in a circle. They usually only contain a few genes. These small DNA circles are easy to insert into bacteria. The plasmid supplied with this kit contains a gene to express the Jellyfish GFP protein.

Also from what you have just mentioned, you need to be able to insert foreign DNA into the donar. This is done by tricking the donar into thinking it is its own DNA.




In the series on Netflix "Unnatural Selection" this guy manages to get a dogs sperm to glow in the dark. Not sure if he ever got a glow in the dark dog, but from watching the series it did not look that complicated. At one point a bio hacker injected himself with a solution to show how it worked.

Maybe i should try contact these guys and ask them how to go about it. Imagine having mycelium that glows so you can spot contamination.Would be an excellent tool for mycology.


Edited by mongoosed (02/21/20 12:56 AM)


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OfflineLactuva
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Re: Crispr. Could this be done with mycelium? [Re: mongoosed]
    #26498115 - 02/22/20 08:14 AM (3 years, 11 months ago)

That kit looks awesome, I'm going to pick it up it just for kicks. Haven't done gene mods since college.

I don't believe this method would work with mycelium. In order the get the DNA into the genome of the fungus you would first have to insert the plasmid into A. tumefaciens cells. This bacteria is capable of integrating its genes with plant cells, should be capable of doing the same to fungus cells.


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Offlinemongoosed
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Re: Crispr. Could this be done with mycelium? [Re: Lactuva]
    #26500956 - 02/24/20 05:46 AM (3 years, 11 months ago)

Quote:

Lactuva said:
That kit looks awesome, I'm going to pick it up it just for kicks. Haven't done gene mods since college.

I don't believe this method would work with mycelium. In order the get the DNA into the genome of the fungus you would first have to insert the plasmid into A. tumefaciens cells. This bacteria is capable of integrating its genes with plant cells, should be capable of doing the same to fungus cells.




So, are you saying you could do it?

There are mushrooms that do glow in the dark. I wonder if it would be easier to go from shroom to shroom instead of jellyfish to shroom. Wish i was more knowledgable about such things.


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Invisiblemurderlabz
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Re: Crispr. Could this be done with mycelium? [Re: mongoosed]
    #26502007 - 02/24/20 05:51 PM (3 years, 11 months ago)

Gene-edited CRISPR mushroom escapes US regulation

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CRISPR-Cas9 assisted gene disruption in the higher fungus Ganoderma species

"CRISPR-Cas9 assisted gene disruption was established for the first time in higher fungi by taking Ganoderma species as typical examples. "



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Efficient CRISPR-Cas9 Gene Disruption System in Edible-Medicinal Mushroom Cordyceps militaris

"CRISPR-Cas9 system that included the Cas9 DNA endonuclease,RNA presynthesizedin vitroand a single-strand DNA template efficiently generatedsite-specific deletion and insertion. This is the first report of a CRISPR-Cas9 system inC. militaris, and it could accelerate the genome reconstruction ofC. militaristo meet theneed for rapid development in the fungi industry"

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Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system

"we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cinerea. Using a high-throughput reporter assay with cryopreserved protoplasts"



Edited by murderlabz (02/24/20 05:59 PM)


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