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Invisiblec10h12n2o
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Re: Diving into agar pouring your own? [Re: Igloomis]
    #26510151 - 02/29/20 06:02 PM (3 years, 10 months ago)

Quote:

CatsLoveHouseMusic said:
So you don’t streak plates? May I ask why? Curious




The pic he posted is a transfer plate not a spore plate. But lots of people don't streak, it's not mandatory by any means. It has lots of benefits tho

Quote:

Igloomis said:
How long until I should expect to see growth, given a 72-ish F incubation temperature?




Growth from spores? Could be 4 days, could be 3 weeks or more. Hard to know. Usually within 10 days


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Invisiblewoofwoof
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Re: Diving into agar pouring your own? [Re: c10h12n2o]
    #26510389 - 02/29/20 08:50 PM (3 years, 10 months ago)

Syringe to agar, could take about 3-5 days to see growth


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OfflineIgloomis
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Re: Diving into agar pouring your own? [Re: woofwoof]
    #26528942 - 03/11/20 10:52 AM (3 years, 10 months ago)

Quick followup.

My plate lids were covered with condensation, so it was very difficult to see what was going on. I probably should have waited a bit longer. I picked out two pinhead-sized isolated colonies and transferred them. I now have two beautiful dishes of well-isolated contaminants! :tongue:

I made another attempt this morning, this time working from a spore print. Before inoculating I turned both top and bottom of petri dishes upside down on an alcohol-saturated paper towel in my SAB and tapped condensation droplets off the lids. Then inoculated and sealed with parafilm.

Let's see what happens this time.


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OfflineMadMyc
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Re: Diving into agar pouring your own? [Re: Igloomis]
    #26528987 - 03/11/20 11:16 AM (3 years, 10 months ago)

Condensation is a battle.  Pouring as close as you to the setting temp helps to cut down on this.  Also can help to keep them in stacks which clears up the condensation for most of the stack.  Sometimes storing them upside down (after they have set!) helps.

condensation :machinegun:


Pinhead size?  A bit too early to tell, did you have any magnification?  Dime-size to quarter-size will let you identify the myc you want


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Offlinejbgtaa
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Re: Diving into agar pouring your own? [Re: woofwoof]
    #26529442 - 03/11/20 04:31 PM (3 years, 10 months ago)

Quote:

woofwoof said:
Syringe to agar, could take about 3-5 days to see growth



Could take up to 2 weeks


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Invisiblewoofwoof
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Re: Diving into agar pouring your own? [Re: jbgtaa]
    #26529469 - 03/11/20 04:50 PM (3 years, 10 months ago)

Quote:

jbgtaa said:
Could take up to 2 weeks




Yes! But lately, mine have been showing growth in the 3 -5 day range.


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OfflineIgloomis
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Re: Diving into agar pouring your own? [Re: woofwoof]
    #26571586 - 04/01/20 04:37 PM (3 years, 9 months ago)

Here's where I am with my first agar attempt with B Plus. This is after 2 transfers from original inoculation from a spore print (so, 3rd generation). It's not monoculture but it looks clean and even, so I'll be taking it to grain in a couple of days.It seems like it has taken a long time, but I guess a week isn't bad for this much development.



Edited by Igloomis (04/01/20 04:38 PM)


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Invisiblec10h12n2o
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Re: Diving into agar pouring your own? [Re: Igloomis]
    #26571595 - 04/01/20 04:43 PM (3 years, 9 months ago)

Definitely not even. I'd do one more transfer at least, if it was me. From as far away from that patch as possible

Itll probably be fine if you dont, but still worth cleaning it up a bit

I'd grab 7 o'clock,  and maybe 12 o'clock and 5 o'clock too, but I like redundancy


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (04/01/20 04:44 PM)


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OfflineIgloomis
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Re: Diving into agar pouring your own? [Re: c10h12n2o]
    #26581886 - 04/06/20 02:35 PM (3 years, 9 months ago)

I meant even as far as no wild fuzzy patches like in the source I took it from. I did one more transfer but I went ahead and inoculated grain with the rest. I did an agar wedge inoculation (never tried that before). I hope it works. If not, there's another transfer right behind it. Oats are cheap :smile:


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OfflineIgloomis
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Re: Diving into agar pouring your own? [Re: Igloomis]
    #26826640 - 07/16/20 09:56 AM (3 years, 6 months ago)

Just a bit of follow-up.

I continued to transfer on multiple lines of dishes, but all of my efforts resulted in green mold, despite extreme care in all aspects of my procedure. At the time I was using only pre-poured plates purchased on the internet. I went back to the drawing board and stepped through my entire process bit-by-bit, eliminating variables: sanitation, technique, sterilization, jars, lids, oats, etc. I finally got around to pouring my own agar and, voila! no more contamination in my oat spawn. I can only conclude that the pre-poured plates (which had a very cloudy appearance compared to those I later poured myself) were the culprits. I have six quarts of spawn going now with good colonization and no sign of the green meanies.

Moral of the story: just pour your own damn plates. If you think you're saving time and cutting corners by paying someone else to do it you might be fooling yourself (and spending a lot more money than necessary). And it's fun once you get the hang of it.


Edited by Igloomis (07/16/20 09:56 AM)


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OfflineC12ShroomMan
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Re: Diving into agar pouring your own? [Re: Igloomis]
    #26828632 - 07/17/20 10:24 AM (3 years, 6 months ago)

Quote:

Igloomis said:
Just a bit of follow-up.

I continued to transfer on multiple lines of dishes, but all of my efforts resulted in green mold, despite extreme care in all aspects of my procedure. At the time I was using only pre-poured plates purchased on the internet. I went back to the drawing board and stepped through my entire process bit-by-bit, eliminating variables: sanitation, technique, sterilization, jars, lids, oats, etc. I finally got around to pouring my own agar and, voila! no more contamination in my oat spawn. I can only conclude that the pre-poured plates (which had a very cloudy appearance compared to those I later poured myself) were the culprits. I have six quarts of spawn going now with good colonization and no sign of the green meanies.

Moral of the story: just pour your own damn plates. If you think you're saving time and cutting corners by paying someone else to do it you might be fooling yourself (and spending a lot more money than necessary). And it's fun once you get the hang of it.




I just went straight to pouring my self, easy and cheap... why not?
The HG containers make it even easier, pour em on the counter let cool, load up in the pc, take em out and crack the lids till condensation clears. ez low effort


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InvisibleDrboomer
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Re: Diving into agar pouring your own? [Re: C12ShroomMan]
    #26829532 - 07/17/20 06:15 PM (3 years, 6 months ago)

A alternative is polypropylene petris if you dislike plastic waste. They are able to be pcd and reused. The trade off is you only get like 80 % of the disposable petris visibility but alot better than pasty plates about the same as holy grail plates. They are fine to see through in person if you shine a flashlight on them but suck for pictures


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