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Offlinedeathcapforcutie
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Registered: 01/15/20
Posts: 69
Last seen: 3 years, 5 months
Agar Lessons... * 1
    #26496938 - 02/21/20 12:28 PM (3 years, 11 months ago)

Today I judged that my agar plates were ready for some transfers.  Here are a few things I've learned so far:

*  When you see pics or videos of experienced people using sterile technique you think, "OK, simple.  Just do X, Y, and Z."  But it takes practice.  I'm still fumbling around the SAB like I was at age sixteen trying to unhook a bra.

*  When the alcohol is inside the box you need it on the outside.  When it's outside you need it in there.  Genius...what if I had TWO bottles?

*  When it comes to inoculating a plate from a MS syringe I truly am an artist. And that artist is Jackson Pollock.  Like snowflakes covering a tiny frozen pond, that shit was everywhere.

*  When you're carefully lowering your "scalpel" (xacto knife) into the pretty white mycelium it's really disheartening to hear the hiss reminding you you forgot to cool it in a less precious part of the dish.  Even more so the second time you do it.  And...the third.

*  Being baked while you're doing transfers...I definitely made the right decision there.

I'm definitely glad I decided to start using agar.  I'm gonna pick up another couple packs of PP5 this weekend.  I never thought standing in my underwear and a pair of surgical gloves slicing up gelatin and breathing alcohol fumes could be much fun but I'm really enjoying it.


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OfflineMycobum7
Stranger

Registered: 01/28/19
Posts: 180
Last seen: 11 months, 9 days
Re: Agar Lessons... [Re: deathcapforcutie]
    #26496950 - 02/21/20 12:43 PM (3 years, 11 months ago)

laughed several times while reading this, this is me to a "t" and youre right it seems simple then you do it and finish and have a list of about a dozen things to do different next time. i would imagine if i had a friend who was a surgeon and i explained the goal he/she would come up with some good protocol / order of operations to go by . good post


--------------------
"This isn't my first rodeo ya know.......its like my third."


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Offlinefeldman114
Stragler


Registered: 09/06/19
Posts: 3,365
Loc: Bravos
Last seen: 3 years, 9 months
Re: Agar Lessons... [Re: Mycobum7]
    #26496961 - 02/21/20 12:54 PM (3 years, 11 months ago)

Haha quality poast:rofl2:
The bra-fumbling analogy really hit home for me lol


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: Agar Lessons... [Re: deathcapforcutie]
    #26496972 - 02/21/20 01:01 PM (3 years, 11 months ago)

Been there and still do dumb shit. Getting super baked is the only way to get quality work done :grin:


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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OfflineEl Chupacabra
Stoned Ape
Male


Registered: 01/07/19
Posts: 676
Loc: 🇺🇸
Last seen: 1 year, 1 month
Re: Agar Lessons... [Re: Diego]
    #26496981 - 02/21/20 01:13 PM (3 years, 11 months ago)

Weed + coffee + music = perfect lab work routine.


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Invisiblestaytrippy420
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Registered: 03/23/13
Posts: 2,337
Loc: Canada Flag
Re: Agar Lessons... [Re: El Chupacabra]
    #26497002 - 02/21/20 01:31 PM (3 years, 11 months ago)

Had me going with this one 😂

Take your time man your gona fuck some shit up at the beginning no matter what, so account for that. I still fuck shit up and I’ve done like 50 plates.. Practise some dry runs if you need to.

You want to learn good habits right off the bat. Watch videos. Read sterile teks.
Study like your cramming for your grade 10 math exam.
I literally go through the motions for like 30min before I even load the sab.

,..And for the record, I always work in the sab stoned. Keeps me calm and collected. :stoned:

Bods got a good video series that helped me along the way.
https://www.shroomery.org/forums/showflat.php/Number/24337011


--------------------
Tek's I use

LAGM2020


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OfflineEl Chupacabra
Stoned Ape
Male


Registered: 01/07/19
Posts: 676
Loc: 🇺🇸
Last seen: 1 year, 1 month
Re: Agar Lessons... [Re: staytrippy420]
    #26497005 - 02/21/20 01:34 PM (3 years, 11 months ago)

Quote:

staytrippy420 said:
I still fuck shit up and I’ve done like 50 plates.



Did you maybe forget a couple 0's? 50 is like 2 whole sleeves lol.


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Offlinepureshrooming
feels like a stranger
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Registered: 05/28/18
Posts: 321
Last seen: 12 days, 5 hours
Re: Agar Lessons... [Re: El Chupacabra]
    #26497100 - 02/21/20 02:33 PM (3 years, 11 months ago)

I stupidly bump the sides of my sab so much, I need to mount a mirror above it so I can give myself this look...



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OfflineMr.Wizard
Harbinger of Hallucination
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Registered: 01/20/20
Posts: 281
Last seen: 18 days, 7 hours
Re: Agar Lessons... [Re: El Chupacabra]
    #26497232 - 02/21/20 03:56 PM (3 years, 11 months ago)

The SAB felt like an old friend when I started working in one! Maybe it's my time in kitchens with raw food rules and such. I also like processes, so who knows.

Watch out with alcohol in the SAB. If you're properly flaming your scalpel you might make a small bomb.


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: Agar Lessons... [Re: Mr.Wizard]
    #26497240 - 02/21/20 04:02 PM (3 years, 11 months ago)

Quote:

Mr.Wizard said:
The SAB felt like an old friend when I started working in one! Maybe it's my time in kitchens with raw food rules and such. I also like processes, so who knows.

Watch out with alcohol in the SAB. If you're properly flaming your scalpel you might make a small bomb.



Haha same goes for Lysol.


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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