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Offlinehuangdeez
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Too much nutrients in my agar?(w/pics)
    #26497082 - 02/21/20 02:24 PM (3 years, 11 months ago)

Whats up? I got a quick question about my agar. Started these on the 15th some are from syringe (the ones with the water stain shit) and some with a print(the ones with the black chunks). This was my first attempt with agar, and it looks really messy, but something is growing on these things. The only issue is that it is growing really slow. Ill put pictures, but could my slow growth issue be due to too much nutrients? These petris are pre poured PDA for fungal growth from amazon.



these first two are the ones I think are contaminated for sure one with bacteria (brown shit) and the other one I'm thinking is cobweb or some other mold (white circle)


this one looks healthy to me? I'm not for sure, but I think there's something wrong since it took almost a week to get this weak ass growth.



and then there's this one. I don't know if that white dot is a contam or not, and I have another plate (not pictured) that looks exactly like that. I am suspicious of these because they're the only two of the 5 petris which were inoculated with a syringe that have anything growing on them.

My 5 plates inoculated with prints all have fuzzy things growing over the chunks of spores. I didn't post them all because they look like the "healthy" example here, thanks in advance for taking the time to help.


Edited by huangdeez (02/21/20 02:25 PM)


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Offlineiwh678
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Re: Too much nutrients in my agar?(w/pics) [Re: huangdeez]
    #26497171 - 02/21/20 03:22 PM (3 years, 11 months ago)

What is the recipe you are following?


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Offlinehuangdeez
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Re: Too much nutrients in my agar?(w/pics) [Re: iwh678]
    #26497175 - 02/21/20 03:24 PM (3 years, 11 months ago)

Quote:

iwh678 said:
What is the recipe you are following?



theyre pre made from amazon. PDA


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Offlineiwh678
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Re: Too much nutrients in my agar?(w/pics) [Re: huangdeez]
    #26497187 - 02/21/20 03:28 PM (3 years, 11 months ago)

Does the label say what and how much its made of?


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Offlinejbgtaa
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Re: Too much nutrients in my agar?(w/pics) [Re: iwh678]
    #26497195 - 02/21/20 03:33 PM (3 years, 11 months ago)

Pour your own plates and leave them for 4 days to see if your sterile technique is on point.

Plates one and 3 are showing solid germination with a small amount of bacteria. Plate 2 has a mold colony and should be trashed, and plate 4 is extremely bacterial with a possible hint of mold. Transfer from 1 and three and trash the rest. Don’t use so many spores next time, they’re microscopic and will still germinate even if you can’t see them.


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InvisiblemushboyMDiscord
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Re: Too much nutrients in my agar?(w/pics) [Re: huangdeez]
    #26497196 - 02/21/20 03:33 PM (3 years, 11 months ago)

Quote:

huangdeez said:
Whats up?







That white fuzz bottom left is a mold colony. The others look ok but scraping spores onto agar is really ...inefficient? Better to streak with a loop or swabs.


Quote:








That's all bacteria from a dirty spore syringe.

Everything is working fine with the agar. It's only been 6days how fast were you expecting the growth to be?


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Offlinejbgtaa
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Re: Too much nutrients in my agar?(w/pics) [Re: mushboy]
    #26497209 - 02/21/20 03:39 PM (3 years, 11 months ago)

I use my scalpel to “streak” plates and usually just touch the print and then the plate even if I don’t see anything on the blade, butttt

Yeah mushboy is right. Use something else besides a syringe next time.


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