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Infinity-25
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Help! Does this jar look healthy? Would you G2G?
#26496449 - 02/21/20 05:12 AM (3 years, 11 months ago) |
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Knocked up on feb 3rd, from clone on agar. Shaken at feb 11th.
Was thinking to let it colonize for a few more days before G2G if healthy. Opinions?
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Ombisha
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Infinity-25]
#26496507 - 02/21/20 06:42 AM (3 years, 11 months ago) |
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So, you want to use this jar as a master culture for g2g transfers? It looks alright to me. If you really want to be sure, you could shake it and wait a few days to see if it recovers. That is the "old school" way of checking for hidden contamination. If you want to use it right away, i say go for it. It looks at, or nearly at, 100% colonization. Even if its not totally colonized, it is not that much of a problem, since you are going to g2g. If you wanted to spawn it to bulk tho, then you should better make sure that it is at 100%.
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El Chupacabra
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Ombisha] 1
#26496571 - 02/21/20 07:53 AM (3 years, 11 months ago) |
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Growth looks pretty weird to me. I would not expand that. My eyes could be playing tricks on me but pics 3 and 4 look especially suspect. I think I can see off color grains with growth that doesn't look like mycelium but I'm not sure. If I spawn that at all I would spawn that solo.
Never g2g jars that aren't fully colonized. Its bad practice that honestly makes no sense and compounds risk.
I'd give it a good shake and see how it recovers.
Edited by El Chupacabra (02/21/20 08:10 AM)
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Ombisha
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Re: Help! Does this jar look healthy? Would you G2G? [Re: El Chupacabra]
#26496600 - 02/21/20 08:21 AM (3 years, 11 months ago) |
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Quote:
El Chupacabra said: Never g2g jars that aren't fully colonized. Its bad practice that honestly makes no sense and compounds risk.
I agree that it is a bad practice to be purposefully doing that, but there are times, where that may be desirable. One could be, for example, low on time and expanding a jar with let's say 90% colonization would be better alternative to waiting another week until one has the time to work on it again. How is there more risk? Other than that the jar will probably take slightly longer and that means slightly more time for contam to appear.
Quote:
El Chupacabra said: I'd give it a good shake and see how it recovers.
I agree, this would be best.
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MrBovineJoni
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Ombisha]
#26496604 - 02/21/20 08:27 AM (3 years, 11 months ago) |
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Quote:
Ombisha said:
Quote:
El Chupacabra said: Never g2g jars that aren't fully colonized. Its bad practice that honestly makes no sense and compounds risk.
I agree that it is a bad practice to be purposefully doing that, but there are times, where that may be desirable. One could be, for example, low on time and expanding a jar with let's say 90% colonization would be better alternative to waiting another week until one has the time to work on it again. How is there more risk? Other than that the jar will probably take slightly longer and that means slightly more time for contam to appear.
Because those remaining uncolonised 1-10% could be contaminated (and quite often are) especially if they are slow to colonise.
So by G2G jar like that where you can't be sure what is going to happen with the last 10% you can nicely spread that contamination to every single recipient jar and completely destroy the whole batch.
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El Chupacabra
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Re: Help! Does this jar look healthy? Would you G2G? [Re: MrBovineJoni]
#26496625 - 02/21/20 08:38 AM (3 years, 11 months ago) |
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Also the master jars get handled quite a bit in the process of doing g2g, it's exposed for a more extended period of time too. With uncolonized grains this just compounds risk with what was already likely to be risky spawn.
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Ombisha
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Re: Help! Does this jar look healthy? Would you G2G? [Re: MrBovineJoni]
#26496627 - 02/21/20 08:39 AM (3 years, 11 months ago) |
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Quote:
MrBovineJoni said:
Quote:
Ombisha said:
Quote:
El Chupacabra said: Never g2g jars that aren't fully colonized. Its bad practice that honestly makes no sense and compounds risk.
I agree that it is a bad practice to be purposefully doing that, but there are times, where that may be desirable. One could be, for example, low on time and expanding a jar with let's say 90% colonization would be better alternative to waiting another week until one has the time to work on it again. How is there more risk? Other than that the jar will probably take slightly longer and that means slightly more time for contam to appear.
Because those remaining uncolonised 1-10% could be contaminated (and quite often are) especially if they are slow to colonise.
So by G2G jar like that where you can't be sure what is going to happen with the last 10% you can nicely spread that contamination to every single recipient jar and completely destroy the whole batch.
I see. Yeah, it is definitely a bad practice. But i can still see how that could be appealing to some.
I personally don't wait additional days after the jar is visually at 100% before g2g, like i would wait before spawning. Even expanding a culture that you are sure is 100% clean is a risk. It depends on how much risk are you willing to undergo. That is perhaps bad advice to be giving out tho.
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Infinity-25
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Re: Help! Does this jar look healthy? Would you G2G? [Re: El Chupacabra]
#26497242 - 02/21/20 04:04 PM (3 years, 11 months ago) |
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Thanks for your input all!
Quote:
El Chupacabra said: Growth looks pretty weird to me. I would not expand that. My eyes could be playing tricks on me but pics 3 and 4 look especially suspect. I think I can see off color grains with growth that doesn't look like mycelium but I'm not sure. If I spawn that at all I would spawn that solo.
Never g2g jars that aren't fully colonized. Its bad practice that honestly makes no sense and compounds risk.
I'd give it a good shake and see how it recovers.
Yeah those pics look a bit suspect, but they look not so much off colour IRL. Anyhow, Im going to shake and see what happens. Healthy recovery time should be 2-3 days usually?
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El Chupacabra
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Infinity-25]
#26497395 - 02/21/20 05:45 PM (3 years, 11 months ago) |
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24-48 hours to recover usually. Faster recovery is best and usually indicates some good healthy spawn. The more sluggish it is to recover the more suspect it is. Also if it's difficult to shake that's another indicator of not great spawn. Lastly, when and if it does become completely colonized shake it up real good and open and give it a whiff. If it smells sweet then you know it's pretty bacterial.
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eLeSDenes
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Infinity-25]
#26497399 - 02/21/20 05:48 PM (3 years, 11 months ago) |
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looks fishy to me, i would scrap it
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Infinity-25
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Re: Help! Does this jar look healthy? Would you G2G? [Re: El Chupacabra]
#26500148 - 02/23/20 04:16 PM (3 years, 10 months ago) |
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Quote:
El Chupacabra said: 24-48 hours to recover usually. Faster recovery is best and usually indicates some good healthy spawn. The more sluggish it is to recover the more suspect it is. Also if it's difficult to shake that's another indicator of not great spawn. Lastly, when and if it does become completely colonized shake it up real good and open and give it a whiff. If it smells sweet then you know it's pretty bacterial.
Thanks!
- Update - This is how it recovered after 36 hours:


And this after 48h (night time, so using artificial light vs daylight previous pics. Looks less white on the photo than reality):



Shaking was not hard. Looks to be recovering to me, but I still have an untrained eye. Just trying to learn and develop a sense of how it should look. Have way more jars on the way  Your opinions?
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Sockadin



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Re: Help! Does this jar look healthy? Would you G2G? [Re: Infinity-25]
#26500151 - 02/23/20 04:17 PM (3 years, 10 months ago) |
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I would not put that to more grain.
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CocaineBuffet
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Sockadin]
#26500195 - 02/23/20 05:07 PM (3 years, 10 months ago) |
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Looks a greyish. I wouldn't G2G that. You could top fruit it and see what happens if you are curious.
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Infinity-25
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Re: Help! Does this jar look healthy? Would you G2G? [Re: CocaineBuffet]
#26500836 - 02/24/20 02:38 AM (3 years, 10 months ago) |
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Quote:
CocaineBuffet said: Looks a greyish. I wouldn't G2G that. You could top fruit it and see what happens if you are curious.
Ok will try that. Just put a casing layer on top? Should I dunk it too then?
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Infinity-25
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Infinity-25]
#26503405 - 02/25/20 03:23 PM (3 years, 10 months ago) |
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I did some more research and see some people are fruiting straight from the jar with questionable jars using a verm or CVG casing. Others put it to small trays mixed with CVG.
Having some trouble deciding what would be best for my jars if I want to see if I can get some fruits from them. Any suggestions on best current (non-outdated) practice?
Thanks y'all for your input
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El Chupacabra
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Infinity-25]
#26503961 - 02/25/20 08:04 PM (3 years, 10 months ago) |
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Invitro would probably be best. Just my opinion. It cuts out the whole breaking it up/birthing process which would require time and energy lost to recover. I wouldn't have high hopes anyway, but Id think chances are best going invitro.
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hippieduster
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Re: Help! Does this jar look healthy? Would you G2G? [Re: El Chupacabra]
#26504086 - 02/25/20 09:14 PM (3 years, 10 months ago) |
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If you question your jar and decide to just fruit it.. do a mini mono.. 1 jar per tub. eliminates the possibility of lost work and sometimes your results end up surprising like multi flush tub.
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Infinity-25
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Re: Help! Does this jar look healthy? Would you G2G? [Re: El Chupacabra]
#26504294 - 02/26/20 02:08 AM (3 years, 10 months ago) |
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Quote:
El Chupacabra said: Invitro would probably be best. Just my opinion. It cuts out the whole breaking it up/birthing process which would require time and energy lost to recover. I wouldn't have high hopes anyway, but Id think chances are best going invitro.
How would one go about that?
- Do I rehydrate the grains somehow first? - When using CVG casing, should it be pasteurized? (I read lots of people do not pasteurize coco these days, but not sure it also works for invitro or if I better pasteurize because of the questionable grain)
@Hippieduster I have a few of these jars. will do a mini shoebox with 1 or 2 of them and see what happens.
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El Chupacabra
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Infinity-25]
#26504394 - 02/26/20 05:22 AM (3 years, 10 months ago) |
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No I wouldn't rehydrate. I've always thrown on a CVG casing and put the lid back on to let it colonize the casing a bit then remove the lid and mist when necessary. Misting is all you need, no need to dunk. I typically just sterilize CVG. You can pasteurize too but I hate to babysit it. I think CVG does fine no matter what.
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Infinity-25
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Re: Help! Does this jar look healthy? Would you G2G? [Re: El Chupacabra]
#26504614 - 02/26/20 10:14 AM (3 years, 10 months ago) |
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Quote:
El Chupacabra said: No I wouldn't rehydrate. I've always thrown on a CVG casing and put the lid back on to let it colonize the casing a bit then remove the lid and mist when necessary. Misting is all you need, no need to dunk. I typically just sterilize CVG. You can pasteurize too but I hate to babysit it. I think CVG does fine no matter what.
Hmm interesting. Thanks for helping me out here.
Whats your opinion on bulk substrate CVG? Do you also sterilize? I read a lot of people do not heat treat it at all anymore. I can see why this would be different from questionable jars and invitro though as we probably are not working with very healthy myc.
From my previous understanding I thought pasteurization was preferable as it would kill/reduce the bad contams and preserving some of the beneficial ones.
Is there a current consensus on preparing casings and CVG? Ive read loads searching around, but not sure if some of the info is outdated now. Maybe someone can link to a up-to-date tek?
Sorry for the many questions haha, I just started growing again and trying to understand and learn. Thanks for your help. I really love this site/community. Its a real treasure of knowledge
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El Chupacabra
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Infinity-25]
#26504746 - 02/26/20 11:56 AM (3 years, 10 months ago) |
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I still would not have high hopes or really any hopes at all, just saying. The mycelium just looks so thin and weak. It looks a lot like plenty of jars ive done that ended up going south. If I were you I would get something else started on agar.
My understanding of coir is that its inert. You dont have to pasteurize or even sterilize from what I've read you can just hydrate it and go. I personally have never tried this because I guess old habits die hard.
For prepping CVG heres a decent link albeit a little old. https://www.shroomery.org/forums/showflat.php/Number/20469910#20469910 Might be better newer threads/posts out there but I doubt theres much of anything of real value that could be expanded upon.
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Infinity-25
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Re: Help! Does this jar look healthy? Would you G2G? [Re: El Chupacabra] 1
#26504989 - 02/26/20 02:37 PM (3 years, 10 months ago) |
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Thanks a lot! Indeed have multiple things going on agar and on jars already. Just gaining experience and trying to make most of what I have. I dont have high hopes for this one no, so just going to try the invitro stuff to get some more experience with the procedures. Some trial and error with the backing power of the shroomery, Much obliged kind sir
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Infinity-25
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Re: Help! Does this jar look healthy? Would you G2G? [Re: Infinity-25] 1
#26545987 - 03/20/20 07:16 AM (3 years, 10 months ago) |
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Update!
I top fruited some of them. 1 was indeed contaminated and had to toss. The other:

Even mixed 2 of them and put to CVG in shoebox. Im amazed that the myc was apparently strong enough to give a good pinset! Im guessing the earlier slow growth was mainly due to too dry grain prep.

Yay for my first grow! Thanks for all your help and suggestions
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