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SpunkyMonkey88
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Registered: 10/08/19
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Re: Ethanol extraction [Re: Darkslide]
#26498792 - 02/22/20 05:23 PM (3 years, 11 months ago) |
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Quote:
Darkslide said:
Quote:
SpunkyMonkey88 said: Ok well I went ahead and bought a liter of everclear but before I do this I wanted to know what is the verdict on how long they should sit in the ethanol for, if I'm not heating it up?
Stamets notes about three days which I'd say is fairly accurate as a minimum. You can let it go much longer.. not that it's needed, but I was comfortable with a week. I'd recommend keeping the crystals in the liquid after straining out the muck. Keeps them from contacting air which will break them down eventually. The entire mix can be stored in the freezer for a very long time.
Ok sounds good thanks for that, I'm going to shoot for a week... Also I forgot to ask how much ethanol per gram?
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Darkslide
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Registered: 11/27/19
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Enough to cover all the material with about a half inch to spare for evaporation. You'll likely lose a little while it sits. Even with a lid. I would also recommend dividing the doses while everything is in liquid. Shake it like you hate it between every pour. I tried a scoop of crystals once and took way too much. It's easy to do.
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endlessnessagain
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Re: Ethanol extraction [Re: Darkslide] 1
#26499427 - 02/23/20 05:19 AM (3 years, 10 months ago) |
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Instead of worrying about different opinions, ask for sources.
Psilocybin is slightly soluble (“difficultly”) in ethanol (Merck index), or practically insoluble in ethanol (Hofmann et al 1958) Psilocybin is readily water soluble. Hofmann 1971
Psilocin is soluble in ethanol (Clarke’s 1986) Psilocin is very slightly ("Difficultly") soluble in water (Merck Index) Psilocin is unstable in solution (Merck Index)
In other words, using ethanol would not work very well to extract psilocybin unless you had a certain amount of water mixed in. There is no test AFAIK of what is the ideal amount of water to be mixed in with the ethanol, but anecdotal evidence shows vodka works. Too little ethanol and too much water could potentially make psilocybin degrade faster (possibly due to more concentrated ethanol inactivating enzymes that dephosphorylate psilocybin?), but too much ethanol would not dissolve well psilocybin.
And extracting psilocin isn't really wanted because it is so unstable.
Hope that helps.
Edited by endlessnessagain (02/23/20 05:21 AM)
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SpunkyMonkey88
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Registered: 10/08/19
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Quote:
endlessnessagain said: Instead of worrying about different opinions, ask for sources.
Psilocybin is slightly soluble (“difficultly”) in ethanol (Merck index), or practically insoluble in ethanol (Hofmann et al 1958) Psilocybin is readily water soluble. Hofmann 1971
Psilocin is soluble in ethanol (Clarke’s 1986) Psilocin is very slightly ("Difficultly") soluble in water (Merck Index) Psilocin is unstable in solution (Merck Index)
In other words, using ethanol would not work very well to extract psilocybin unless you had a certain amount of water mixed in. There is no test AFAIK of what is the ideal amount of water to be mixed in with the ethanol, but anecdotal evidence shows vodka works. Too little ethanol and too much water could potentially make psilocybin degrade faster (possibly due to more concentrated ethanol inactivating enzymes that dephosphorylate psilocybin?), but too much ethanol would not dissolve well psilocybin.
And extracting psilocin isn't really wanted because it is so unstable.
Hope that helps.
Yeah dude I've googled "solubility of of psilocybin and psilocin" too... But there seems to be soo much conflicting experience reports so I thought I'd ask here...
I am just currently soaking an 8 gram single dose to test this method... if it works, sweet if not what ever it's just 8grams...
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Darkslide
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Registered: 11/27/19
Posts: 166
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Should be just about ready  I think you'll find the Everclear to work the best. It seems to have just the right amount of water and alcohol. In the link I provided earlier http://www.fanaticus.com/mycoalki.htm you'll note some reports from some extremely reliable sources. Several of which are old friends so I trust their opinions and reports. Most notably RR (under his Mycotopia spelling), Soliver, Hippie3 (RIP), Shedthemonkey and Suckerfree. Check out who SF wrote the letter to. There's more to that convo, but it's tucked away in a Mod forum.
but nothing speaks better than personal experience.. so hit it man!
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SpunkyMonkey88
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Re: Ethanol extraction [Re: Darkslide]
#26510798 - 03/01/20 05:26 AM (3 years, 10 months ago) |
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Quote:
Darkslide said: Should be just about ready  I think you'll find the Everclear to work the best. It seems to have just the right amount of water and alcohol. In the link I provided earlier http://www.fanaticus.com/mycoalki.htm you'll note some reports from some extremely reliable sources. Several of which are old friends so I trust their opinions and reports. Most notably RR (under his Mycotopia spelling), Soliver, Hippie3 (RIP), Shedthemonkey and Suckerfree. Check out who SF wrote the letter to. There's more to that convo, but it's tucked away in a Mod forum.
but nothing speaks better than personal experience.. so hit it man! 
Quote:
Darkslide said: Should be just about ready  I think you'll find the Everclear to work the best. It seems to have just the right amount of water and alcohol. In the link I provided earlier http://www.fanaticus.com/mycoalki.htm you'll note some reports from some extremely reliable sources. Several of which are old friends so I trust their opinions and reports. Most notably RR (under his Mycotopia spelling), Soliver, Hippie3 (RIP), Shedthemonkey and Suckerfree. Check out who SF wrote the letter to. There's more to that convo, but it's tucked away in a Mod forum.
but nothing speaks better than personal experience.. so hit it man! 
Yaup I soaked 10 gs for about 5 days strained and filtered it then I filtered it and soaked it in everclear again but boiled it for a few hours this time then I combined the 2 and boiled it down to 10 mls.. it was the cleanest trip I had ever had! Absolutely no regrets! I needed it to, cus I feel like my last 5 experiences have been ruined by unbearable body loads and stomach discomfort. There was seriously none of that with this! I am a believer.
So I wonder though is it the 5% water in the ever clear doing the heavy lifting because everything I read says psilocin and psilocybin are not soluble in ethanol but ever clear obviously works just fine...
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Darkslide
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Registered: 11/27/19
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SpunkyMonkey88
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Re: Ethanol extraction [Re: Darkslide]
#26515320 - 03/03/20 07:27 PM (3 years, 10 months ago) |
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So whats the deal with the sugars/starches that dissipate? I know they are not actually psilo crystals...so if I did a cold filter to get them out of the jar do you think I would be loosing a significant amount of actives? Is it possible for psilo to settle and sit among the sugar crystals at the bottom of the jar?
Does that make any sense?
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MurderByNumbers
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SpunkyMonkey88
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Not sure why you referenced that page, it has nothing to do with making an extract at least from what I gathered by browsing at it...
But anyway I decided to make a tincture with the rest of my fungus. I have 40 grams sitting in ethanol right now and has been in there for more than 5 days. My plan was to boil the concoction in a double boiler today, then filter it and repeat. But instead of letting the pulp sit for another 5 days I was going to boil it for 2 hrs. I did this last time and it worked well.
I would like to filter out those “crystals” that settle at the bottom IF they aren’t active... I would hate to loose any actives though so does anyone know for certain that I wouldn’t loose much psilocybin by cold filtering out those crystals and then running a few hundred mls of ethanol afterwards to get anything left behind in the filter... How significant of a loss would I take by doing it this way?
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Darkslide
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Registered: 11/27/19
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I'm not a chemist, but my guess is the magic is bound in the crystals.. whatever they are, and floating freely in the solvent. Before the crystal debate people, including myself, were scooping the crystals out and munching on them. Very crunchy. Don't chew if you value your teeth.  However, one could also drink the blue juice leaving the crystals in the bottom achieving the same effect. I finally left it at a good evap reduction and made it a point to shake the mixture before divided doses. You can sep the crystals but they loose potency fairly fast. Most likely oxidation. I've always found it better to cover them in reduced solvent and store in the freezer. Funny story there. When they bashed in PF's door and searched his house, one of the searchers pulled the jar out of the freezer and put it back not knowing what it was.
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MurderByNumbers
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Quote:
SpunkyMonkey88 said: Not sure why you referenced that page, it has nothing to do with making an extract at least from what I gathered by browsing at it...
It lists multiple species and their measured concentrations of psilocybin and psilocin. (dry)
It lists out methods of preservation, extraction, % of actives extracted, conditions stored at, how long, degradation over time, etc.
Edited by MurderByNumbers (03/10/20 01:33 AM)
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poisoned
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I'm making shroom moonshine every year for festivals. I usually use some moonshine that's at around 45% and just let the shrooms soak in that. Then add some lemons and sugar, so that thing can pass as "limoncello".
I'm usually aiming to get 1gram/shot. Does it work as good as 1g of shrooms? I don't think so, but when you have plenty of shrooms, it doesn't matter that much.
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ReverendMyc

Registered: 03/29/19
Posts: 1,496
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Re: Ethanol extraction [Re: poisoned]
#26575416 - 04/03/20 03:29 PM (3 years, 9 months ago) |
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Hey SpunkyMonkey
Congrats on a successful first run. I have a solid Green Dragon recipe that I have used to extract pot in Everclear or 151 rum (Everclear is way more effective). So I am looking forward to experimenting with psilocybin to perfect another recipe. It looks like you used 10 grams of mushies in your first run. Was that fresh or dried? Fresh seems low and dried seems very high if you did the whole thing in one trip. Or possibly the psilocybin transfer from solids to liquid was not complete. So did you do the full 10 grams of dried in one go? Was it the equivalent of a ten gram trip? (Which I have no frame of reference for, by the way.)
I always used a double boiler for my dragon recipe. Keeping the temperature precisely was always paramount. The most efficient transfer of thc to ethanol takes place around 155 to 160 degrees Fahrenheit. But ethanol boils at 170 f so you really need to watch it closely. Now I have a water bath and Pyrex media jars, so I am looking forward to the next batches and trials.

Did you ever get to test crystal extraction?
Thanks for being a pioneer.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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