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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
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Quote:
DontPlay said: Iv'e been wanting to try agar cultures for a while, but hate having to buy more supplies.
I tried my first organic honey liquid culture following a tek. And inoculated it with a syringe. The jar has about 600ml in it and I pumped about 2cc's into it. After a week I am already seeing good mycelium growth.
Only thing is the syringe was pretty thick with spores and their are still alot of spores sitting on the bottom of the LC. Not sure if they are going to germinate.
you still dont know if your LC is clean or not until you do some test jars which will take another 2-4weeks.
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Pastywhyte said: Agar can be made from the same ingredients as LC, just need to add agar. If your determined that LC can work and agar is too hard then fine. LC can work, and people can win the lottery. No one is trying to steer you wrong, we are try to help you gain success. Do a search on contamed LC and have a look at how many people felt the same as you did and learned the hard way.
Personally I would never inoculate a LC with anything but a clean agar wedge 
QFT. and why use a wedge in LC when you can go right to master jar. especially with a nice isolate 
if you want to advance in this hobby just like any other hobby you need a lil cash to buy new equipment to step your game up to the next level.
which means investing in a PC for insured success in all aspects of home mycology
Edited by twistedty (12/05/13 03:33 PM)
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DontPlay
Yeah Science!



Registered: 12/13/07
Posts: 750
Loc: On Land most of the time....
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Just want to make it clear that obviously the more precautions you take using sterile techniques and a pressure cooker are most certainly going to yield better results and less problems with contamination.
But I do like to experiment and see what I can get away with going low-tek Just to prove it can be done lol.
Twistedty "you still dont know if your LC is clean or not until you do some test jars which will take another 2-4weeks."
You are right about that, I will post my results when I make a few test jars. Worse case scenerio it doesn't work out no harm no foul.
-------------------- My Trade List I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. Hunter S. Thompson
I prefer dangerous freedom over peaceful slavery. ~Thomas Jefferson
Edited by DontPlay (12/05/13 04:03 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Quote:
twistedty said: QFT. and why use a wedge in LC when you can go right to master jar. especially with a nice isolate 
if you want to advance in this hobby just like any other hobby you need a lil cash to buy new equipment to step your game up to the next level.
which means investing in a PC for insured success in all aspects of home mycology
Agreed. LC for cubensis is pretty much pointless.
OP the reason people assume that you have a PC is because if your planning to use advanced culturing techniques, you better get one. Even if you just want do get a LC for use in cakes, you need to step your game up. The equipment can be a little pricey when just starting, but once you have that equipment just growing is pretty cheap. Not too mention there are cheap ways to find things like a PC if you search around, it don't have to be new or the best.
As for making LC with steaming and spores, you can sometimes luck out and steam an LC successfully. Sometimes you can luck out inoculating LC with spores and tissue. The success rate for one of those practices is usually a coin flip. To do both has an extremely low chance of success tho IMO.
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Dirtygoat
Bread



Registered: 10/22/13
Posts: 561
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Thanks guys.. And I know, I know lol, like I said I will buy a PC eventually lol, I just have to be sure this is a hobby I can continue, not just a one time batch thing.
And I read somewhere that as soon as you get a spore syringe, it would be a good idea to make LC from the start so that the single syringe lasts you longer.. I already used up two syringes, and I hope to successfully make some spore prints, but in case I don't, I was looking for an easy way out to increase the amount I can inoculate with the last two cc's of spore solution I have.
Again, thanks everyone, you all more than answered my first few questions.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
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Quote:
Dirtygoat said: Thanks guys.. And I know, I know lol, like I said I will buy a PC eventually lol, I just have to be sure this is a hobby I can continue, not just a one time batch thing.
And I read somewhere that as soon as you get a spore syringe, it would be a good idea to make LC from the start so that the single syringe lasts you longer.. I already used up two syringes, and I hope to successfully make some spore prints, but in case I don't, I was looking for an easy way out to increase the amount I can inoculate with the last two cc's of spore solution I have.
Again, thanks everyone, you all more than answered my first few questions.
you must of read an OLD POST saying making an LC out of a MS syringe.
buying a PC steps your mycology to a whole new level period.
unless you just want to do brf ms cakes your whole mycological career.
even RR says LC is for noobs.
once you learn brf cakes either youre happy with that and thats all you want to do, or you step your game up to agar.
its up to you and how many fruits you want to produce
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
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Quote:
Dirtygoat said: Does agar need a PC?
yes. if you dont have a pc dont even bother worrying about agar just stick with making your cakes only.
there is nothing to advance to if your cant afford a pc.
just do cakes
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
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this is a hobby just like any other and requires money to advance. i dont mean to sound like a dick sorry
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poopymannn
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Registered: 12/24/19
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thank you
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poopymannn
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yup
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poopymannn
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yeah
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poopymannn
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yup
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poopymannn
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yeah
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poopymannn
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yes
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