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OfflineOmbisha
Transmutant
I'm a teapot User Gallery


Registered: 02/17/20
Posts: 667
Loc: Tartaria
Last seen: 1 year, 3 months
"White Grass Jelly" - Agar Substitute
    #26494482 - 02/20/20 06:25 AM (3 years, 11 months ago)

The synopsis:

Firstly, i will say that White Grass Jelly is probably not better than agar, but it works well enough. The measurements have to be adjusted though.
I did not intend on using it, but agar proved hard to get in my country. I had to go to the Vietnamese/gastronomy marketplace to look for it and honestly thought i got it... until i realized i had not, but i was already 20 plates in by that time.

Anyway, here is what i got:



Besides the baggie containing the powdered white grass jelly, there was also some kind of seeds and a pack of sugar in the package... 
:tryingnottodie:

You can notice, that in the "ingredients" section it says 15% starch. What i draw from that is that you should probably use 15% less nutrients in your recipe, but i did not bother.


The Measurements:

If you follow the agar prep teks with white grass jelly, you will get a goopy mess. I learned that quickly. What i found out is that you want to use roughly three times the amount that the teks recommend. The first batch i made with 9 grams per 400ml didn't even settle...

So for the second batch i used:
15g White grass jelly
400ml Water
6g Brf

Translates to 37.5 grams per Liter.
This was a goopy mess too, but proved ok for germinating spores... It was a pain in the ass to cut from tho.


For the third batch i used:
20g White grass jelly
200ml Water
100ml Water from soaking rye
15 drops of dr. Oetker gel food coloring (not important)

Translates to 66 grams per Liter.
This one was far better. Settled nice and was easy to cut from. The growth also seemed much more healthy, but that was probably because of different nutrients.


In the future, i will probably try something like 80g White grass jelly per Liter... That is what i would probably recommend to you, but i have not tested it yet, so as of now i recommend 66g/L.
As far as i can tell, you can use it in any agar prep tek you choose. What i did is:
-Heated it all in a pot and stirred it for it to dissolve
-Poured it all in my glass bottle
-Pressure cooked it for 45 minutes at 10psi
-Poured my plates in a still air box


Here are the plates:

37.5g/L + brf (its overgrown now)


66g/L + grain soak water




Last words:

I am not recommending to use white grass jelly instead of agar, i am simply telling how to do it. Finding something like this would have been helpful to me in the past, so i figured that i would type it out for someone in the same position.

Thank you for reading. Enjoy your days.
-Ombisha


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