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OfflineAresCeres
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Seeking monoculture advise
    #26493649 - 02/19/20 04:06 PM (3 years, 11 months ago)

Hey guys ,

Just starting to isolate my B+ , I basically started out with MS and now working my way to a rhizomorph monoculture but planning to perform a LC in the meantime to inoculate few tubs while I work on the process.
Just wondering if my tubs are going to be a MS mess if I make LC with these dishes and how many transfers am I looking at to get a decent monoculture - Kindly advise



A2 would ne my pick for LC


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InvisibleInfiniteDreams
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Registered: 10/25/19
Posts: 1,224
Re: Seeking monoculture advise [Re: AresCeres]
    #26493657 - 02/19/20 04:09 PM (3 years, 11 months ago)

A2 would be your best pick for a monoculture.

Just grow it out and use a clone to get a monoculture quicker.


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OfflineJohnRainyII
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Registered: 02/07/20
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Re: Seeking monoculture advise [Re: AresCeres]
    #26493884 - 02/19/20 06:33 PM (3 years, 11 months ago)

As far as Ive gleaned, a true monoculture takes many, many isolations to get and is rarely done in home cultivation.

I think what most of us here go for is an isolation of a complex of strains that produce well for us, but it's not a monoculture.  Even clones from a mushroom is  a complex of strains, according to these message boards.


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OfflineSockadin
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Re: Seeking monoculture advise [Re: JohnRainyII]
    #26494084 - 02/19/20 08:59 PM (3 years, 11 months ago)

Who is the TC that did like 24 transfers and counting.... I cant remember the name but it is an impressive read. 2 years and still going looking for that mono.....


Check out Stropharis on isolating sectors thought.


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OfflinecronicrFacebook
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Re: Seeking monoculture advise [Re: JohnRainyII]
    #26494100 - 02/19/20 09:19 PM (3 years, 11 months ago)

Quote:

JohnRainyII said:
As far as Ive gleaned, a true monoculture takes many, many isolations to get and is rarely done in home cultivation.

I think what most of us here go for is an isolation of a complex of strains that produce well for us, but it's not a monoculture.  Even clones from a mushroom is  a complex of strains, according to these message boards.



getting a monoculture is easier than most would like to believe.
A2 would be a good choice for isolation , A would be my choice to grow out, diversity is key when looking for clones to grow


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineSnowtrooper
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Registered: 11/17/19
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Re: Seeking monoculture advise [Re: cronicr]
    #26494592 - 02/20/20 07:50 AM (3 years, 11 months ago)

Hey bro
Thanks for your input,
So you would suggest to take a clone from every sector on disk A and then isolate each of those disks to monoculture then make LC of each or at least grow out each one to see the one that performs best.
Is that a solid plan for this culture?

Thanks for your time


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OfflineThe Mycologist
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Re: Seeking monoculture advise [Re: Snowtrooper]
    #26494616 - 02/20/20 08:06 AM (3 years, 11 months ago)

You get agar going.

Once you have clean growth you put that on seeds.

The shrooms that grow will have a large variety of genetics, grab a sexy looking fruit and put a piece on agar.

Now you can make transfers from this clone to achieve a monoculture much quicker.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflinecronicrFacebook
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Re: Seeking monoculture advise [Re: Snowtrooper]
    #26495158 - 02/20/20 01:05 PM (3 years, 11 months ago)

Quote:

Snowtrooper said:
Hey bro
Thanks for your input,
So you would suggest to take a clone from every sector on disk A and then isolate each of those disks to monoculture then make LC of each or at least grow out each one to see the one that performs best.
Is that a solid plan for this culture?

Thanks for your time



I would just put some to grain and grow it out, from there take clones and slant them if they perform well.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineSnowtrooper
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Registered: 11/17/19
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Re: Seeking monoculture advise [Re: cronicr]
    #26495329 - 02/20/20 02:31 PM (3 years, 11 months ago)

Nice one


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OfflineSockadin
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Re: Seeking monoculture advise [Re: Snowtrooper]
    #26495331 - 02/20/20 02:32 PM (3 years, 11 months ago)

Why do I keep seeing the word Seeds? Am I missing something over the last 10 years? Are we planting mushrooms now?


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OfflineSnowtrooper
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Re: Seeking monoculture advise [Re: The Mycologist]
    #26495334 - 02/20/20 02:35 PM (3 years, 11 months ago)

Brilliant bro thanks


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Invisibletramalot
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Re: Seeking monoculture advise [Re: Snowtrooper]
    #26495529 - 02/20/20 04:00 PM (3 years, 11 months ago)

All grains are seeds!


--------------------
It's the Journey I enjoy!


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OfflineSockadin
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Re: Seeking monoculture advise [Re: tramalot]
    #26495543 - 02/20/20 04:06 PM (3 years, 11 months ago)

Not all seeds are grains.


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Offlineantmanmax
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Registered: 03/22/19
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Re: Seeking monoculture advise [Re: Sockadin]
    #26495551 - 02/20/20 04:10 PM (3 years, 11 months ago)

Quote:

Sockadin said:
Why do I keep seeing the word Seeds? Am I missing something over the last 10 years? Are we planting mushrooms now?




Usually I hear it in reference to WBS. Technically oats, rye, and wheat are berries, not seeds, but they contain seeds.


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