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Hemperor
Stranger

Registered: 02/19/20
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Agar transfer progress
#26493563 - 02/19/20 02:58 PM (3 years, 11 months ago) |
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Hi everyone!this is my first post in this community. I've been in this hobby for a bit, but I've just recently gotten into agar......I've started quite a few agar dishes but it always seems like my 2nd or 3rd transfer ends up taking very long. growth is much less vigorous.and loses its ryz. growth. im changing nutrients in between transfers and I don't know why. I just want to isolate a substrain.....also can someone tell me how to upload pictures from my phones photo gallery
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ichugwindex
Dex



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Re: Agar transfer progress [Re: Hemperor]
#26493595 - 02/19/20 03:16 PM (3 years, 11 months ago) |
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Ok on the top left of the screen next to your name there is a button that says "pics" click the pics button and on the next screen there will be an "upload" button once you click the upload button it should be pretty easy from there
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Mr.Wizard
Harbinger of Hallucination



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Re: Agar transfer progress [Re: Hemperor]
#26493601 - 02/19/20 03:20 PM (3 years, 11 months ago) |
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Can you be more specific about your details, please? IE how did you change your nutrients? That will affect their growth, it's completely changing what they eat. They will use up all the old stuff before going to the new. Some folks will cut the agar off the bottom of their transfers so there's less material for them to use there, and force them onto the new plate.
Make sure to check out the agar teks, at least Bod's comprehensive. You will learn that many isolates suck, and it could be better to clone a good fruit from a grow before trying to isolate. At least be aware that if you're just wanting 'one' isolate, you should have like 10 isolates to fruit, and see which the best ones are.
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c10h12n2o
serial dilutor



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Re: Agar transfer progress [Re: Mr.Wizard]
#26493607 - 02/19/20 03:27 PM (3 years, 11 months ago) |
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might be a good idea to read bods guide and/or my guide in my sig
post pics and we can help you out
changing nutrients is going to change how the myc looks, if you want consistency you need consistent recipe
id just use standard MEA (20g agar, 14-15 g malt extract per liter)
the same myc can go from rhizo to fluffy or vise-versa on different mediums. often tomentose myc will go rhizo when it hits grains
why do you want to isolate a strain? if you are new you should just be shooting for clean myc. if you do want to play with isolates it makes very little sense to only do one, you need to test LOTS to find a good one
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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InfiniteDreams


Registered: 10/25/19
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Re: Agar transfer progress [Re: c10h12n2o]
#26493644 - 02/19/20 04:02 PM (3 years, 11 months ago) |
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To throw some newbie perspective on this:
from what I read it seemed like 20g agar 20 malt extract was standard (e.g. 2% agar 2% nutrient).
Looking into doing 3/4 nutrient like you suggest, a 1.4% to 1.5%.
I got rhizo even with 2% nutrient though. I think this is highly dependent on genetics though, I did not get 0% or 100% rhizo with this formula, more like 66%.
And I have germ plates that are showing rhizo after 2+ weeks. There are transfers that I thought were battling contams, but now it just seems like rhizo sectors vs tomentose areas.
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c10h12n2o
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the standard recipe is actually 30g malt extract, 20g agar per liter
but we typically use half strength MEA at 14-15g malt extract, 20g agar per liter
rhizo vs tomentose is dependent on lots of things, including agar recipe, genetics, conditions, etc
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Hemperor
Stranger

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Re: Agar transfer progress [Re: Hemperor]
#26494457 - 02/20/20 06:03 AM (3 years, 11 months ago) |
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I've had several plates that grow out very well,so I can get clean mycelium..what I mean by changing nutes I'll start with mea on 1 plate then use pda on the second,so I don't lose genetic strength,but I've also used the same nutes when transfering..I really only want an isolate because I've had many successful grows and this seems to be the next step in my journey.I'm prob. gonna start some clones then.. Thank you guys for the responses I will def. check out bods comp. guide to see if I am missing something
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c10h12n2o
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Re: Agar transfer progress [Re: Hemperor]
#26495030 - 02/20/20 12:00 PM (3 years, 11 months ago) |
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You'd probably get a lot out of my guide as well
I understood what you meant about changing nutrients. There is no need to worry about losing genetic strength within as few transfers as you are doing. That would be well after 100 transfers. Stick to a recipe if you want consistency
Keep in mind what you are doing: expanding mycelium. Same thing you do on grain or bulk sub, but on a smaller scale. If losing genetic strength isnt an issue on a bulk sub, it definitely wouldnt be on much much smaller expansion
I think clones are more suited to your purpose. If you are gonna go for isolates, make at least 5 or 10 and test them all. Only doing one is like picking a random person and expecting them to be good at basketball
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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