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Offlinejackrabbitcytoplas
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Registered: 01/30/20
Posts: 129
Last seen: 3 years, 6 months
Contamination id
    #26489857 - 02/17/20 11:49 AM (3 years, 11 months ago)

So in the interest of wasting agar training until I have spores from a reputable source I have contaminated some agar with the gills of a dried active and I'm trying to learn to identify the contams.
(I'm also cloning supermarket oysters on agar with no visible contams so far and also on cardboard :smile:)

Sorry for the shitty images, I tried to get the best light/focus and that's the best I could without opening the containers also sorry for the long post.

Would appreciate any help identifying those.


It's yellowish-brown in the center and whitish mycelium.
No idea what it is, Aspergillus?
Just to make sure, it's pure contam and nothing to do with the source mushroom, right?




Same dish, the Pacman looking one is the one from before, the other one is green with water droplets resting on it, powdery white around it and very faint mycelium around that.
Looks like Penicillium to me.


This one looks like a dried up black/green scab.
No idea what it is. :confused:


This one is very airy and cottony while all the other ones are perfectly flat, looks like the kind of mold you get on vegetables you leave outside for too long.

Thanks in advance for any help. :grin:


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OfflineMrBovineJoni
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Registered: 11/28/19
Posts: 264
Last seen: 14 days, 1 hour
Re: Contamination id [Re: jackrabbitcytoplas]
    #26491079 - 02/18/20 02:48 AM (3 years, 11 months ago)

The first three are all molds for sure - I can't tell you which ones exactly as I typically don't care about the type of mold on my cultures; just that it is there in the first place.

That last one could be oyster mycelium but I have no experience with oysters personally and the pic is very blurry. Oyster mycelyium growth is white, strong and can be quite often be fluffy looking but that is me repeating someone else. If you are not sure what that is - transfer and see if it can be cleaned up.


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Offlinezoidbergo
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Registered: 06/10/13
Posts: 257
Loc: Central Coast, California
Last seen: 4 hours, 53 minutes
Re: Contamination id [Re: MrBovineJoni]
    #26493499 - 02/19/20 02:17 PM (3 years, 11 months ago)

Judging by the color they're probably Cladosporium in the first pic and Penicillium in the next two. Very common molds. The last pic is too blurry to tell if its mold or mushrooms mycelium. You can't ID mold without a microscope though.

Regardless, identifying your contaminants wont actually help. Just chuck 'em.


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