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OfflineSockadin
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose] * 1
    #26519246 - 03/05/20 06:09 PM (3 years, 10 months ago)

I got you!

Let me put it another way. less is more. There are millions of spore  in your syringe. All you need is one little drip per hole, the more you squirt the higher your contamination rate is because all syringes are dirty . It is inhertent in the way spore prints and syringes are made.

Also all you need are two spores to germinate in each inoculation point. The more spores you out in each hole the more diverse set of genetics you might get and because of the way Mycelium structures are created you end up mixing the genetics to the point where you have a higher randomness of good fruiting vs poor fruiting genetics.

Imagine playing in a lottery pool of 1 out of 50 vs 1 out of 100,000,000 your odds are way better with less.

I hope that helps.

Any TC's want to jump in and help clarify if that didn't make sense.. Bod, Mushboy...


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OfflineLSA Woodrose
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: Sockadin]
    #26519327 - 03/05/20 06:42 PM (3 years, 10 months ago)

Quote:

Sockadin said:
I got you!

Let me put it another way. less is more. There are millions of spore  in your syringe. All you need is one little drip per hole, the more you squirt the higher your contamination rate is because all syringes are dirty . It is inhertent in the way spore prints and syringes are made.

Also all you need are two spores to germinate in each inoculation point. The more spores you out in each hole the more diverse set of genetics you might get and because of the way Mycelium structures are created you end up mixing the genetics to the point where you have a higher randomness of good fruiting vs poor fruiting genetics.

Imagine playing in a lottery pool of 1 out of 50 vs 1 out of 100,000,000 your odds are way better with less.

I hope that helps.

Any TC's want to jump in and help clarify if that didn't make sense.. Bod, Mushboy...




Okay, let me see if I understand what you're saying. Basically, if I can find a way to be exceptionally light-handed and precise, that a mere drop or two should be all I need? Moreover, you're actually saying something counter-intuitive in that I wouldn't just reap the benefits of more spores left over due to being miserly, but that less content from the needle is actually better and more conducive to growing mushrooms? If I am misrepresenting what you're saying, please tell me. If not, that's some pretty amazing stuff!

By the way, I think I am cooked for the day. I really am not up to inoculating tonight. The work seems to require too much precision for me to attempt this while not at my best. Hopefully this doesn't harm the substrate in my jars, which has already been sterilized 11 hours.


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OfflineSockadin
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose] * 1
    #26519341 - 03/05/20 06:47 PM (3 years, 10 months ago)

Jars will last week's or months if sterlize properly.

You are totally spot on understanding what I am saying. It would be interesting to see if others had an opinion on this topic.

  Wait till tomorrow to inoculate if your tired. Safety first!


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OfflineLSA Woodrose
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose]
    #26520056 - 03/06/20 06:26 AM (3 years, 10 months ago)

Hey all, I hope you're all having a great morning. Sorry in advance for the HUGE wall-o-text. I also have to apologize in advance for the very nature of what I am about to ask.

Okay, I woke up this morning ready to start preparing for inoculation, and I had an experience, and I need to ask perhaps the most important question about growing mushrooms I could ever ask, and it has nothing to do with teks, substrates, and any of the million questions I have been asking. I was laying in bed for about an hour in the dark, before the sun started coming up, and it hit me like a bat to the face. The second I inoculate my first jar hole, thanks to our draconian drug laws, I share the same status as the the guy cooking meth in a lab. This has occurred to me, but only sort of tangentially during this whole learning process of getting ready to grow, and even gathering supplies and making the substrate jars. I guess I was so busy and so excited at the coolness of this, that I never really let the legality of all this be my dominant thought.

But this morning, it also occurred to me that nothing I have done so far is illegal. Weird, but true.

Feel free to flame, but I am actually having a huge crisis of faith, mostly in our absurd legal system. But I am a middle aged guy, have no desire to sell drugs of any kind. Ever. But that wouldn't stop over-zealous prosecutors and cops from using the weight I grow for personal use as evidence of intent to traffic.

I know that to most or all of you, this is going to sound crazy paranoid, but I am having a lot of trouble getting myself to take this huge step, which only yesterday, it was a foregone conclusion I would. Because the moment I do, I have crossed a line from law abiding citizen to felony activity. I'm sure NYC cops have much better things to do than worry about my small cache of inoculated, colonizing substrate jars and personal mushrooms in my fruiting chamber. But you know that headspace you get into while tripping and even smoking weed. How your mind can start to really go down that rabbit hole of fear, knowing that even though the chances are probably absurdly unlikely of anything bad happening, the legal penalties for this are huge. Even if I never sell or give away a single shroom.

I never thought of myself as such a pussy, but do any of you growing and consuming your own stash, or giving/selling it to others, ever feel like this is a really, really bad idea? Yesterday I couldn't wait to inoculate, see my jars get to 100% colonization, birth the cakes, wait for my harvest, and then reap its vast benefits. Now, I am literally unable to will myself to inoculate that first jar. Even though almost NOBODY gets busted for shrooms who isn't hawking them out in the open at a Phish concert with a 2 pound bag in his knapsack, or the guy who has a vast network of customers and ends up selling ten pounds to an undercover DEA Agent.

This may only be some temporary paranoia. But I am possessed by this feeling I can only describe as being paralyzed by fear at the fact that inoculating that first hole puts me on the other side of some very oppressive, despotic laws. According to our stupid, ridiculous, crazy drug laws, one drop of MS into a substrate jar, and I become the legal equivalent of Walter White.


Have any of you ever experienced anything like this?

By the way, I am well aware that maybe this site is the wrong place to ask questions like this, as I'm sure most people here have made their peace with any risks to our freedom that this poses. So go ahead, tell me I'm nuts. Tell me I've come this far, you have all bent over backward to help me through this step by step, and I'm too stupid to live. I have come so far on this journey, and now I am stopped at that line between not only what is legal and not, but the fact that Mushrooms are actually classified worse than opiates, because our system of jurisprudence doesn't even see them as medically useful. Even though they are. So how do I push myself past this wall and move forward or turn and back away slowly, forsaking all I have done to this point? If I was 18, I probably wouldn't give a crap. Because I thought nothing could hurt me back then.


And as always, sorry for the huge wall-o-text. I probably could have said all of this in a paragraph or two. So, anyway, come at me. Flame and insult me all you will. I'll gracefully take whatever verbal assault you want to hurl at me. If for no other reason than if I end up unable to push past this fear, I will essentially deserve it for wasting all your time.


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OfflineSockadin
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose] * 1
    #26520072 - 03/06/20 06:44 AM (3 years, 10 months ago)

Some people who post on this site do so from countries where there are places laws or no laws against possession or cultivation of magic mushrooms.

Really no one can answer this question for you. It is a choice you have to make based on the understanding of your location and the laws of your place of residence.

All we can do is show you the door. The choice is up to you. You can always order medicinal spores and grow food.


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OfflineLSA Woodrose
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose]
    #26520087 - 03/06/20 06:58 AM (3 years, 10 months ago)

I know, Sockadin, and I know I'm officially an idiot and a jerk. I have the right to waste my own time and my own money, but I have no right to waste all of your time. I am about to lift weights to try and clear my head, maybe go out on another bicycle ride for the same purposes. My kitchen is cleaned, ready, and prepped, my SAB minutes away from being ready to go. All I have to do is shower, put on clean clothes, put all my hair into a doo-rag, and alcohol off all my work surfaces again, then grab my hypos, and just do it. I don't know why the ramifications of severe legal entanglements and prison time never really dawned on my until just now.

Sorry everyone. I am no happier about being stuck at this horrifying road of indecision.

Well, if I end up doing it, after my lift and my ride today, I will have a fun tripping party, all food and party-favors on me, for anyone near the NY City or Tri-State area who wants to join. Though, now that I have basically proved myself as being a f*cking headcase, I don't foresee a lot of takers.


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OfflineRandalf the Grey
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose] * 1
    #26520105 - 03/06/20 07:15 AM (3 years, 10 months ago)

I can't tell you to go past that wall. Nor can I tell you to turn around. The legal aspect is something that everyone has to deal with on their own.
Their is no way to do what we do without risk. The only thing you can do is minimize that risk. Tell no one, or extremely few, of what you are doing. It's one thing if a friend knows you can get mushrooms, jts another thing for them to know you're growing them. Most people will not rat their friends out over something small but you never know what will happen when someone is faced with losing their kids, their job, felony charges and years in prison.
These days , thankfully, this one doesn't carry the weight it once did but if you smoke weed there is a chance it's legal for you now but if you are like me you have been smoking it since before it was legal. Yes the charges you would face are different but the precautions are the same, just more so. I think back to when I started smoking weed as a preteen (yeah, I was that kid). Tell no one except those you smoke with because they also have something to lose. Don't flaunt it. As much as I would love to walk around in a Shroomery hoody with the psil. molecule and the shroom logo, but it give police a reason to watch me closer so I don't. When throwing away suspicious waste, don't triple wrap and tape it and throw it in a dumpster behind some store at midnight wearing all black. Just calmly walk to your trash can and throw it away. If you are worried about your trash being searched, don't put it on the curb until trash truck in on the street. If it is something that isn't just suspicious but incriminating and you don't want it in your trash at all,public parks. Public parks have trash can. Those trash can are public trash can. You can throw all the trash you want in them, not just trash made at the park. I have a park in walking distance, but you can just take your dog\gf\bf\kids\self for a walk in the park and as you are leaving, just drop the bag of undesirables in the trash.
Old substrate can be buried\mixed in with ground\soil or put in flower pots. I threw a bunch in a 15gal flower pot and during warmer months I get a few extra fruits out of it.
Tub are easy to hide or repurpose, same with jars. I use them as drinking glass, storage containers,etc.
You will never be able to say "I am not at risk" in this hobby until laws are amended or repealed. Until then, you just have to decide if you feel comfortable being responsible for your safety.
The more you do it and experience these practices, taking these ideas from theory to application, the more comfortable you get and the easier it all is. Both a cult and the safety sides of it. But don't let that slip too far to the point of complacency.
I believe you mentioned doing some extractions. Sorta the same thing. The seeds and plants may not be restricted but the second your start the extraction process it becomes a legal conundrum. Thanks to the analog act, even non controlled substance become illegal if portrayed as such. I.E. selling oregano as weed will still get you charged for selling weed; selling blotter dipped in water as LSD is still a felony.

I'm not here to bash you over the head for being concerned about your future. You do not owe any of us anything. You asked questions and we volunteered to help. What happens from this point is entirely up to you. I don't want to push you one way or the other, but I do understand where you're coming from and personally I think it might just be the fact that you were on the verge of doing something new that is causing such intense anxiety over the issue. Keep it in perspective, please don't take this the wrong way but I'd be willing to bet this is not the first illegal thing you've done and will probably not be the last.
I personally do kind of hope that you stick with it. You are a very thorough in your research oh, you asked good questions, you take advice appropriately, and I would like to see your grows develop as you learn from this community. I know how much I changed from my first PF jar to my one-year mark, and would love to see that change in you as well. Do not let my desire to see someone learn sway your decision.

If you do decide to not go through with it, and do not have any friends that would be willing to take it over, maybe consider making swabs from your syringes to give to the community. There are lots of spore giveaways and contests in the trade forum. Always some new people, or people returning to the hobby that need to restart looking for some fresh spores. I don't say this because I want them. But rather as a way to help others in your position and to get some use out of the syringes.

You're not the first one to get nervous or anxious or cold feet. And you are certainly not in the wrong for taking very serious and deliberate consideration of your future. It is actually that concern that makes me think you have a good grasp on the full scope of the situation and are capable of making rational and educated decisions. I can't tell you what you should do, and no one else can either, it's all about what you feel you are capable of.

Edit:holy crap I didn't mean to write that much. Voice to text....haha,


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


Edited by Randalf the Grey (03/06/20 07:24 AM)


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OfflineLSA Woodrose
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: Randalf the Grey]
    #26520145 - 03/06/20 07:44 AM (3 years, 10 months ago)

Quote:

Randalf the Grey said:
Edit:holy crap I didn't mean to write that much. Voice to text....haha,





No, no! This was precisely the kind of measured, thoughtful, intelligent and understanding post I was looking for. Please, whenever you respond to me, remember that I am not one of the people who takes issue with long posts, particularly when filled with thoughtful insights. I am in the middle of lifting and am going to go out on my bicycle the second I'm done, to clear out the cobwebs. I already feel a lot better after the lift, and I hope that I can push through this fear of Joe Law, who in all honesty, probably could give two royal-shits about some 50+ year old guy growing shrooms in his basement for 100% personal use. Especially in a city like The Big Apple, where the cops have much, much more pressing criminal issues. I bet I could walk into my local precinct, chat up some cops, and accidentally tell them I am growing shrooms, and they would probably tell me to watch my back and not give it a second's thought. NOT that I would be that stupid, mind you.

I am going to read your post again, probably several times. But please, I beg you, DO NOT ever apologize to me for writing long posts. I appreciate the length.

Edit: By the way, Randalf, is that S next to your name meant to depict that you're a site sponsor? If so, regardless of how my current lunatic insanity goes, shoot me a PM.


Edited by LSA Woodrose (03/06/20 07:48 AM)


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Offlinefootpath
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose] * 2
    #26520195 - 03/06/20 08:30 AM (3 years, 10 months ago)

The 'S' is a supporter account - those generous enough to give some money to the site in exchange for gradient usernames and unlimited uploads.. and maybe some other features I'm unaware of.

In regards to your worries, you kind of just have to do your best with discretion and come to terms with the actuality of the situation - you're growing some mushrooms. That's all you're doing.
People of the general public, "upstanding citizens" break many laws all the time and many of them are endangering or somehow directly impacting others... so, in the grand morality of it all, you're really doing no harm.
Could it impact you poorly? For sure. The odds are small if you're smart about it, but it is a risk you have to be conscious of and willing to accept.

Not to mention, it's really easy to only need to grow for a couple months to supply yourself with over a year of mushrooms. So, you don't have to be doing it all the time. That's what I did for the longest time... grow for three months/close up shop for 18 months, etc.


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OfflineRandalf the Grey
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose] * 1
    #26520213 - 03/06/20 08:45 AM (3 years, 10 months ago)

I am very glad to hear you appreciate my ranting and rambling.:smile: I absolutely love learning and seeing\helping others learn. I am by no means and expert, in anything really, but rather a jack of all trades. In the words of Bill Nye, everyone you will ever meet knows something that you don't. I really try to take that to heart and the exchange of ideas and information is essential to growth. We get stagnant when we only entertain our own thoughts and ideas.

As for the S, I am not a sponsor, just a supporter. I started my cult journey at the beginning of last year (January 4th I believe) and didn't actually make an account until several months later. Only a few weeks after that was what I call The Great Shroomery Outage of 2019. There was a security breach and to protect the site's\users' info they pulled the plug and shut the whole thing down until security changes could be made to eliminate the vulnerability. In that time, there was panic. Subreddits and the FB (not affiliated officially with forum) blew up with people desperately looking for the teks and ratios they use. It was down for 6 or 7 weeks I think and a lot of people were stuck in the middle of a grow with no info on what to do next. I had already started taking note and was able to share some with people but this is what cemented my decision to create my book of Genesis both as a grow log and a reference for teks and ratios and agar recipes and such. The idea being that if Shroomery were to go down again, I could simply hand this book to someone and they would have all the info they need to start from scratch with spore and go all the way to fruiting with only the info in the book.
After a couple weeks, Shroomery admins got the word out about what had happened and that they were working to fix the issue but it was require upgrades to the system which costs money and takes time. I can't do anything about the time but seeing how valuable this place is to not only me but so many others, and just how great the community is (I fucking love the people here) I knew I wanted to support them financially if I could. Once the site came back up, they brought back the supporter accounts and immediately bought one.
It also helps as I tend to upload a buttload of pic, especially in my journal where I just upload pic of my log instead of retyping it all.

Real quick, going back to you safety concerns....
I rent my house, buying just isn't an option right now. In my lease it says the property management company can enter the house for maintenance and such but must give at least 24 hours notice (usually give 48 or more). I keep things pretty inconspicuous and while they can't look in any of my stuff, I can't deny them access to any room in the house. I have kept reptiles since I was a teen and often build very large enclosures for them. So.... I built a large cabinet;6'x4'x6'. I painted it and put some decorative molding on the sides so while it looks homemade, I just kinda looks like a large armoire or gun cabinet. I put a latch and a lock on the from and boom, all of my cult supplies can be put in the cabinet in 10 mins or so and requires a warrant to open. My house only has one closet so an armoire story fits, or gun cabinet, or a place for all my sex toys, it really doesn't matter what your reasoning is, it's just a cabinet. I have had an agent from property management company in my house once since I built it and some people for a showing who were looking to buy the house. No one even questioned it. Is this perfect? Nope. Could some people find it suspicious? Possibly, but that's why I like the sex toy story. Most people would not question further. Haha. It's just one more way to minimize risk. Most of my cult stuff stays in there. Pretty much everything but my SAB and lab stuff, which isn't an issue. So if a pipe bursts when I'm not home and someone has to come in, my only concern is gonna be wether I left any weed laying out, and not if they are going to see my mush cult. Again, not perfect, and not the solution for everyone, but in my specific situation it is a big part of minimizing risk factors that are out of my control. I may not be able to stop them from coming in, but I can control what they see while inside.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


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OfflineDemetrius18
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: footpath] * 1
    #26520278 - 03/06/20 09:34 AM (3 years, 10 months ago)

Most of what's been needed to be said, has already been said. That being said (lol), the consequences of being discovered by the wrong crowd are undeniably morose. However you have to compare it with the chance of anyone catching wind, which is relatively low to non-existent unless you put yourself in cross hairs by slipping information. This would be my youth talking but the laws are set so harshly as a scare tactic, at least by my belief. People would face your exact conundrum. You would have to evaluate your risk factors and what might cause anyone to see, or enter your dwelling and see. If you can't smell it see it or hear it, it ain't there.

Example: Apartments can be inspected by the landlord with a single day notice, so an apartment would not be ideal unless the project was disguised very well.

A couple years ago, I had a loud stinky and bulky exploit, it probably took up over a tenth of the floor space of the room, and was ongoing for the better part of 6 months. There was hot air being blown out of a modified window exhaust, and was not quiet. Other than the noise being slightly questionable from directly outside the room, it went without a hitch. Neighbors were not even in the equation, housemates were none the wiser, as it went 24/7 for 6 months. There has not been any consequence from that project which by comparison is like an air horn vs a bike horn. This is an account to help you get some scope to the matter.

Im pulling this last bit out of my ass, but with all of the decriminalization, medical research, and exploration into the subject. Given the strictly personal use, I think a good attorney could significantly help you, idk the classification, but it sounded like class 1 if you were accurately speaking of it earlier, you might be able to argue it no longer fits the classification, as it's beginning to have medical value, is not addictive, and hardly fits the the classification of schedule 1 (if that is in fact where it stands).

Whatever natural disasters cause discovery for you, in court they would sentence you for a schedule 1 charge, but it doesn't meet the definition of schedule one, so it could not be a fair trial until it was reclassified.

But tbh unless you have unwanted traffic inside your dwelling, and it isn't sitting out in the open where it could be seen from a window or open door that's pretty much half of it right there. Good luck, and it's 'X' variety of gourmet mushroom if it ever does come to light.


--------------------
This account is purely theoretical, I will be king of the pirates!


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OfflineLSA Woodrose
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose]
    #26520813 - 03/06/20 03:17 PM (3 years, 10 months ago)

Hey again, everyone! Thanks for all your responses. Well, after lifting weights and doing some cycling around The Big Apple, I decided to just go ahead and inoculate. Screw it. Below, see some pics I took of my setup and post inoculation. Meanwhile, I have some questions if you don't mind. I am going to pass out for a little while, but rest assured, I will respond to EVERYONE who I haven't responded to yet. You all have my profound gratitude.

1. Most of my jar holes now have holes in the microspore tape. Should I reapply more tape over said holes, or just leave the jar holes without tape over them?

2. Assuming I did everything right, how long before I start seeing the characteristic white, cottony colonizing in the earliest of its stages?

3. If I screwed up and green mold starts growing in some or all of the jars, I assume this will happen relatively quickly? It seemed like in RogerRabbit's videos it took a few days only. But I may have misunderstood his explanation.

4. Because of my paranoia this morning, I waited until about an hour and a half ago to start inoculating. Based on what Randalf told me, I assume this shouldn't be a problem? To clarify, I inoculated 30 hours after turning off the burners when the sterilizing was done.


Comments:

It took a little while to get a feel for the inoculating and how much to press the syringe. I started with 6 and 6 jars in the SAB. To be inoculated evenly with Golden Teacher and Costa Rica. My touch for the first few GT jars was way too heavy. I ended up using about 5 cc's for the first 6 jars. Turns out it wasn't my "touch," per se. The actual problem was that bright light I put behind the SAB on the table. About halfway through my first 6 CR jars, I realized this, and shut the light off, only using the bright overhead kitchen florescent light. this made all the difference. I should have realized that a light behind my SAB actually obscured my ability to see the MS enter the substrate on the side of the jars at each hole. So I was over-pumping at the beginning. Oh well, live and learn. Once I could see better, all of a sudden one little drop was easy to spot, and I started really stretching the MS.

If you look at the pictures, you'll see that I have a lot more CR than GT left over, which I expected since the light screwed me up more with the first 6 jars of GT and by the third CR inoculated jar, I started hitting a really nice groove, hands nice and soft, and I inoculated all 24 jars with a decent amount to spare. I was hoping for 5 cc's each left over, but all things considered, I am happy I caught my lighting problem early enough to get through all 24 jars with plenty left.

I did remember to flame sterilize the needle between every jar. Almost forgot more than once, but I caught myself each time.

The Tyvex sleeves felt good and seemed like they worked to help keep air currents out. I also wiped the needle with an alcohol-wet paper towel after every jar, and before flame sterilizing it.


Starting Setup - Same workspace as I used yesterday: 6 jars each to be inoculated by both spores. Cheat Sheet to left.
 

12 Jars in reserve, waiting to be inoculated:



Closeup of inside of SAB pre-inoculation:


Another pre-inoculation closeup:


Leftover spores - notice CR has more fluid than GT. I re-labeled them and put them back in the fridge:


Post-Inoculation picture


Edited by LSA Woodrose (03/06/20 03:23 PM)


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OfflineSockadin
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose] * 1
    #26520915 - 03/06/20 04:21 PM (3 years, 10 months ago)

I'm just happy to see you have spores left over.


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OfflineDemetrius18
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: Sockadin] * 2
    #26521496 - 03/07/20 12:18 AM (3 years, 10 months ago)

Nice job man!


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OfflineLSA Woodrose
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: Sockadin]
    #26521573 - 03/07/20 02:38 AM (3 years, 10 months ago)

Thanks guys! Just re-posting my questions:

1. Most of my jar holes now have holes in the microspore tape. Should I reapply more tape over said holes, or just leave the jar holes without tape over them?

2. Assuming I did everything right, how long before I start seeing the characteristic white, cottony colonizing in the earliest of its stages?

3. If I screwed up and green mold starts growing in some or all of the jars, I assume this will happen relatively quickly? It seemed like in RogerRabbit's videos it took a few days only. But I may have misunderstood his explanation.

4. Because of my paranoia this morning, I waited until about an hour and a half ago to start inoculating. Based on what Randalf told me, I assume this shouldn't be a problem? To clarify, I inoculated 30 hours after turning off the burners when the sterilizing was done.


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Offlinefootpath
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose] * 2
    #26521608 - 03/07/20 03:29 AM (3 years, 10 months ago)

Quote:

LSA Woodrose said:
Thanks guys! Just re-posting my questions:

1. Most of my jar holes now have holes in the microspore tape. Should I reapply more tape over said holes, or just leave the jar holes without tape over them?
The dry vermiculite layer is providing all of your protection from contamination - you can leave them as they are.

2. Assuming I did everything right, how long before I start seeing the characteristic white, cottony colonizing in the earliest of its stages?
Depends on a lot of factors,  but usually within a week.

3. If I screwed up and green mold starts growing in some or all of the jars, I assume this will happen relatively quickly? It seemed like in RogerRabbit's videos it took a few days only. But I may have misunderstood his explanation.
It will start out white, but, yes, usually grows more quickly than cube mycelium.

4. Because of my paranoia this morning, I waited until about an hour and a half ago to start inoculating. Based on what Randalf told me, I assume this shouldn't be a problem? To clarify, I inoculated 30 hours after turning off the burners when the sterilizing was done.
I think you'll be okay.




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OfflineSockadin
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose] * 2
    #26521696 - 03/07/20 06:20 AM (3 years, 10 months ago)

Quote:

LSA Woodrose said:
Thanks guys! Just re-posting my questions:

1. Most of my jar holes now have holes in the microspore tape. Should I reapply more tape over said holes, or just leave the jar holes without tape over them?
Yes the dry verm is your filter.
2. Assuming I did everything right, how long before I start seeing the characteristic white, cottony colonizing in the earliest of its stages? 3-15 days depending on the age of the spores.

3. If I screwed up and green mold starts growing in some or all of the jars, I assume this will happen relatively quickly? It seemed like in RogerRabbit's videos it took a few days only. But I may have misunderstood his explanation.Just because you see mold, doesn't mean you did anything wrong because spore syringes are dirty by nature. Just the luck of the draw. I have had good clean cultures for microscopy from vendors and I have also gotten highly bacterial syringes.

4. Because of my paranoia this morning, I waited until about an hour and a half ago to start inoculating. Based on what Randalf told me, I assume this shouldn't be a problem? To clarify, I inoculated 30 hours after turning off the burners when the sterilizing was done.


If your jars were properly sterilized they will be fine for weeks. Most people inoculate to fast before the insides cool. You are fine. Also can we see a picture of one of the jars where you inoculated?


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OfflineLSA Woodrose
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: Sockadin]
    #26521734 - 03/07/20 06:59 AM (3 years, 10 months ago)

Quote:

Sockadin said:
Just because you see mold, doesn't mean you did anything wrong because spore syringes are dirty by nature. Just the luck of the draw. I have had good clean cultures for microscopy from vendors and I have also gotten highly bacterial syringes.





Ah good to know. LMAO I intuitive felt like this was true, but was honestly hoping it wasn't the case. Oh well. I guess a follow-up question is, even if the needle is dirty, wouldn't the flame sterilization take care of this? Or are you referring not just to the needle, but the inner surface of the plastic chamber and the actual 10cc's of spore-liquid being infected with unwanted pathogens? 

Quote:

Sockadin said:
If your jars were properly sterilized they will be fine for weeks. Most people inoculate to fast before the insides cool. You are fine.





Great! I figured I was needlessly worried about having waited thirty hours after sterilizing to inoculate. But thanks for confirming it!


Quote:

Sockadin said:
Also can we see a picture of one of the jars where you inoculated?





Unfortunately, unless I did something wrong so my jars don't look right, this won't illustrate anything. Before I inoculated, I spent a few minutes re-studying the RogerRabbit video, not only the procedure, but the exact way that he did everything. From the way he twirled the needle over the flame, to the way he let it cool for a second. I made a painstaking effort to even hold the syringe the exact way he did. Turns out this was a good idea, because once I got smart and turned my 6500K light off, and was able to see better with my reading glasses plus the kitchen's ceiling light fixture, I could actually see the drop(s) entering the substrate. I was able to see the drop expand out a little. I was going to try and take a picture of this while doing it, but it seemed like a bad idea, both to break my inoculating rhythm, and pull both arms out of the SAB and have to re-sterilize my Tyvek sleeves and rubber gloves. Plus pulling my arms in and out, more than was absolutely necessary for flaming the needle, would have possibly caused increases in air current that could have been deleterious to my inoculating work. It just seemed like a really poor idea, even thought I will admit I was tempted to stop, take a pic, then get back to it. Sorry for the long answer, but now a day later, those wet spots have dried, and the inoculated substrate looks uniform throughout. I wish I had thought to take a picture right after inoculating. I really should have. But I forgot while taking the other pics.


Edited by LSA Woodrose (03/07/20 07:09 AM)


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OfflineLSA Woodrose
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose]
    #26521748 - 03/07/20 07:12 AM (3 years, 10 months ago)

By the way, Sockadin, in your experience, if you have jars that end up developing mold in them, does that usually mean that it’s not going to just be one or two jars out of the group, but all or most of them? Or can you have one or two bad apples, with the rest being good?


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OfflineLSA Woodrose
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Re: I Need A Lot of Help to Start Cultivating Mushrooms - Total N00b Here! [Re: LSA Woodrose]
    #26522696 - 03/07/20 06:58 PM (3 years, 10 months ago)

Just bumping this up to get some opinions/answers on my last post. :wink:


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