Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]
Invisiblefilthyknees
no coincidence
 User Gallery

Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: How to grow and consume Lions Mane mycelium? (moved) [Re: iwh678]
    #26497200 - 02/21/20 03:35 PM (3 years, 11 months ago)

I wasn't suggesting it is a microdose because it's grain. Simply that it's far less potent than an extract would be for instance. Clearly I think.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


Extras: Filter Print Post Top
Offlinelight4x
Stranger

Registered: 01/07/19
Posts: 110
Last seen: 1 year, 9 months
Re: How to grow and consume Lions Mane mycelium? (moved) [Re: iwh678]
    #26497299 - 02/21/20 04:55 PM (3 years, 11 months ago)

Quote:

iwh678 said:
Quote:

filthyknees said:
Clearly there are different levels of effectiveness associated with different proccessing. Anyone with more than extremely basic research and mushroom knowledge would understand the myceliated rice may be more like a microdose, very clearly a different product than whole mushroom and of course extracts are MUCH different and for different applications.




Tomorrow I'm going to start a 1 gallon liquid culture to get idea of what kind of yields you can from this method. I'll keep this thread updated on the progress.




Can't wait to see


Extras: Filter Print Post Top
Offlineseagu

Registered: 03/03/18
Posts: 952
Last seen: 8 days, 9 hours
Re: How to grow and consume Lions Mane mycelium? (moved) [Re: light4x]
    #26497480 - 02/21/20 06:34 PM (3 years, 11 months ago)

drain and mix is with ice cream afterwards


--------------------
Plan to win or you are planning for failure. Don't let anyone tell you you can't do it. Just figure out the solution. Even if that means banging your head on a wall until the solution oozes out of you.


Extras: Filter Print Post Top
OfflineFungusam
Stranger
 User Gallery

Registered: 05/26/18
Posts: 19
Last seen: 1 year, 9 months
Re: How to grow and consume Lions Mane mycelium? [Re: light4x] * 1
    #26498371 - 02/22/20 12:02 PM (3 years, 11 months ago)

Have you seen this research paper about different liquids to use for the culture?
Here


Extras: Filter Print Post Top
Offlinemush_love_83
Curative Mushrooms Owner
Male

Registered: 02/22/20
Posts: 1
Loc: Virginia Beach
Last seen: 4 months, 27 days
Re: How to grow and consume Lions Mane mycelium? [Re: Fungusam]
    #26498706 - 02/22/20 04:07 PM (3 years, 11 months ago)

I have heard of many people using coffee grounds to grow mycelium.  I also think that you could go the easy route and get a pre-made Lion's Mane grow kit and just harvest the mycelium before it starts to have pin heads pop up.

After that you will need to break of as much of the substrate as possible and dry up the mycelium.

Here's a good article I found on growing edible mycelium.

Also, here's a good link I found on how to grow you own from scratch.

Hope that helps!

Best Regards...


Extras: Filter Print Post Top
Offlineiwh678
Budget Mycologist
Male


Registered: 01/22/14
Posts: 396
Loc: USA
Last seen: 1 year, 6 months
Re: How to grow and consume Lions Mane mycelium? [Re: mush_love_83]
    #26498767 - 02/22/20 05:06 PM (3 years, 11 months ago)

If you are trying to consume the mycelium that's really not a good way to go about it. I imagine eating coffee grounds would be pretty unpleasant. Also lions mane is grow on sawdust so eating a kit would also not be recommended.

People grow on grain for consuming because it is a food product to begin with.


Extras: Filter Print Post Top
Offlineiwh678
Budget Mycologist
Male


Registered: 01/22/14
Posts: 396
Loc: USA
Last seen: 1 year, 6 months
Re: How to grow and consume Lions Mane mycelium? [Re: iwh678]
    #26499589 - 02/23/20 08:55 AM (3 years, 10 months ago)

I ended up innocultaing two half gallon jars each with with 1.3 L of 4% LME.

I'm still new to making liquid cultures so I wouldn't be too surprised if I run into issues with these.

I also may end up doing g a few more quart jar with different mixes. I'm thinking I'll try a 2% LME LC and probably a grain water LC. Does anybody have experience with LC mixes that work well with links mane?


Extras: Filter Print Post Top
OfflineWayward Place
Stranger
Registered: 02/26/20
Posts: 2
Last seen: 3 years, 9 months
Re: How to grow and consume Lions Mane mycelium? (moved) [Re: iwh678]
    #26505343 - 02/26/20 06:24 PM (3 years, 10 months ago)

I would like to comment on the breakingnof the cell wall issue. Now i am a noob but i am not dumb. As i understand it the mycelial structure is like a continous straw. So, if the mycelium, with or without rice ir starch, were broken in the grinding process, the the ends of each fragment of the straw-lime structure would be open for stomach acids to digest. If this is true, then the breaking of the cell wall, unless it co tains a valuable chemical, would be a pointless issue because the acids in your digestive track would hollow out the mycelial structure from both ends.

If i am wrong, please tell me and explain why.


Extras: Filter Print Post Top
Offlinelight4x
Stranger

Registered: 01/07/19
Posts: 110
Last seen: 1 year, 9 months
Re: How to grow and consume Lions Mane mycelium? (moved) [Re: Wayward Place]
    #26505455 - 02/26/20 08:02 PM (3 years, 10 months ago)

Quote:

Wayward Place said:
I would like to comment on the breakingnof the cell wall issue. Now i am a noob but i am not dumb. As i understand it the mycelial structure is like a continous straw. So, if the mycelium, with or without rice ir starch, were broken in the grinding process, the the ends of each fragment of the straw-lime structure would be open for stomach acids to digest. If this is true, then the breaking of the cell wall, unless it co tains a valuable chemical, would be a pointless issue because the acids in your digestive track would hollow out the mycelial structure from both ends.

If i am wrong, please tell me and explain why.




I can't say if you are wrong or right, but I hope you are right because that would make it a lot easier.


Extras: Filter Print Post Top
Offlinelight4x
Stranger

Registered: 01/07/19
Posts: 110
Last seen: 1 year, 9 months
Re: How to grow and consume Lions Mane mycelium? [Re: iwh678]
    #26505456 - 02/26/20 08:03 PM (3 years, 10 months ago)

Quote:

iwh678 said:
I ended up innocultaing two half gallon jars each with with 1.3 L of 4% LME.

I'm still new to making liquid cultures so I wouldn't be too surprised if I run into issues with these.

I also may end up doing g a few more quart jar with different mixes. I'm thinking I'll try a 2% LME LC and probably a grain water LC. Does anybody have experience with LC mixes that work well with links mane?




How they looking?


Extras: Filter Print Post Top
Offlineiwh678
Budget Mycologist
Male


Registered: 01/22/14
Posts: 396
Loc: USA
Last seen: 1 year, 6 months
Re: How to grow and consume Lions Mane mycelium? [Re: light4x]
    #26507621 - 02/28/20 05:16 AM (3 years, 10 months ago)

Not much to update yet. I'm sure I'll see some growth soon.


Extras: Filter Print Post Top
Offlinethebucketmouse
Stranger
Registered: 02/27/20
Posts: 2
Last seen: 3 years, 10 months
Re: How to grow and consume Lions Mane mycelium? (moved) [Re: bodhisatta]
    #26507896 - 02/28/20 10:00 AM (3 years, 10 months ago)

I agree with you that most of the Host Defense products are worthless. Do you have any links to the independent research you referenced? Would love more proof to bolster this argument since I'm having to convince people pretty regularly that his celebrity does not mean his products are good.


Extras: Filter Print Post Top
Offlinelight4x
Stranger

Registered: 01/07/19
Posts: 110
Last seen: 1 year, 9 months
Re: How to grow and consume Lions Mane mycelium? (moved) [Re: thebucketmouse]
    #26507981 - 02/28/20 11:01 AM (3 years, 10 months ago)



Extras: Filter Print Post Top
InvisiblePinback
Stranger
 User Gallery

Registered: 07/20/02
Posts: 836
Loc: Europe
Re: How to grow and consume Lions Mane mycelium? (moved) [Re: light4x]
    #26509244 - 02/29/20 05:31 AM (3 years, 10 months ago)

The study referenced in that blog post was payed for and performed by Fungi Perfecti, with help of an external company. It is not independent. Link here.

The paper is of low quality in my opinion. It does not in any way show any efficacy of their products (which is a separate point from the quality issues).


Extras: Filter Print Post Top
OfflineSolipsis
m̶a̶d̶ disappointed scientist
Male User Gallery


Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands Flag
Last seen: 5 months, 18 days
Re: How to grow and consume Lions Mane mycelium? [Re: dub rules]
    #26509718 - 02/29/20 12:26 PM (3 years, 10 months ago)

Quote:

dub rules said:
I am also not sure whether it is necessary to lyse the cells, but the supplements that Stamets sells under the Host Defense name are freeze dried. That process includes freezing and thawing under vacuum which I am assuming would cause the cells to lyse.




No, freeze drying avoids the formation of ice crystals of the water present. And those ice crystals are what are responsible for screwing up tissue with regular freezing. And yes the freeze-thaw cycles.

Fungal cell walls are not only composed of chitin but also of polysaccharides which can be dealt with in various ways. The question is how this destabilizes the matrix and how big a molecule can be absorbed out of the cells how easily.



If we eat dried mushies we trip, right? But if you eat bigger chunks and don't chew well, its absorbed over a lot more time and the trip is weaker or rather stretched out.
Pulverized mycelium should be pretty good but freeze-thawing before drying and powdering may help make compounds more available but could potentially also mess up their structures so this is the tradeoff we are presented with, apparently. It can also make compounds more liable to degradation (for example oxidation) break down faster as it is not as well protected anymore inside the cells.

I know i am not staff, but idk which forum would be better for the discussion. It is about preparation of medicinal mushrooms, the angle is not to talk about chemistry tho sometimes it is hard to avoid.


Extras: Filter Print Post Top
Offlineiwh678
Budget Mycologist
Male


Registered: 01/22/14
Posts: 396
Loc: USA
Last seen: 1 year, 6 months
Re: How to grow and consume Lions Mane mycelium? [Re: Solipsis]
    #26509968 - 02/29/20 04:15 PM (3 years, 10 months ago)

You know the examples with actives is great. I don't know why that didn't cross my mind. I don't plan on doing anything other than drying and then powdering once I filter the mycelium from this LC.

One draw back to the LC method I anticipate is you don't know 100% what your growing until you test it on agar. With grain its pretty hard to mistake. A lot of these trials could end up trash and you wouldn't realize until the last step.


Extras: Filter Print Post Top
Offlinepzar
Amateur
I'm a teapot User Gallery

Registered: 02/22/20
Posts: 25
Last seen: 7 months, 5 days
Re: How to grow and consume Lions Mane mycelium? [Re: iwh678]
    #26522076 - 03/07/20 11:19 AM (3 years, 10 months ago)

Any updates (probably a little too soon still)? Mostly just wanted to let you know that I'm definitely following this thread and looking for the right tek myself to get some LM myc in me :P

Hope your LC is growing nicely!


Extras: Filter Print Post Top
Offlineskywhisp
Student
I'm a teapot User Gallery

Registered: 11/11/19
Posts: 31
Loc: Australia
Last seen: 3 years, 6 months
Re: How to grow and consume Lions Mane mycelium? [Re: pzar]
    #26533463 - 03/13/20 08:36 PM (3 years, 10 months ago)

Bumping to see how LC goes.

Also, did a quick google and there is some evidence that humans should be able to digest chitin?

Paoletti, M. G., Norberto, L., Damini, R., & Musumeci, S. (2007). Human gastric juice contains chitinase that can degrade chitin. Annals of Nutrition and Metabolism, 51(3), 244-251.

Abstract:
Chitin digestion by humans has generally been questioned or denied. Only recently chitinases have been found in several human tissues and their role has been associated with defense against parasite infections and to some allergic conditions. In this pilot study we tested the gastric juices of 25 Italian subjects on the artificial substrates 4-methylumbelliferyl-beta-D-N,N',diacetylchitobiose or/and fluorescein isothiocyanate (FITC) chitin to demonstrate the presence of a chitinase activity. Since this chitinase activity was demonstrated at acidic pH, it is currently referred to acidic mammalian chitinase (AMCase). AMCase activity was present in gastric juices of twenty of 25 Italian patients in a range of activity from 0.21 to 36.27 nmol/ml/h and from 8,881 to 1,254,782 fluorescence emission (CPS), according to the used methods. In the remaining five of 25 gastric juices, AMCase activity was almost absent in both assay methods. An allosamidine inhibition test and the measurement at different pH values confirmed that this activity was characteristic of AMCase. The absence of activity in 20% of the gastric juices may be a consequence of virtual absence of chitinous food in the Western diet.

That is an old reference too, so there should be some more updated stuff somewhere if people want to look.


Extras: Filter Print Post Top
Offlineskywhisp
Student
I'm a teapot User Gallery

Registered: 11/11/19
Posts: 31
Loc: Australia
Last seen: 3 years, 6 months
Re: How to grow and consume Lions Mane mycelium? [Re: skywhisp]
    #26533477 - 03/13/20 08:42 PM (3 years, 10 months ago)

Another reference for a group finding genetic evidence of mammalian chitinase.

Mareike C Janiak, Morgan E Chaney, Anthony J Tosi, Evolution of Acidic Mammalian Chitinase Genes (CHIA) Is Related to Body Mass and Insectivory in Primates, Molecular Biology and Evolution, Volume 35, Issue 3, March 2018, Pages 607–622, https://doi.org/10.1093/molbev/msx312

Abstract:
Insects are an important food resource for many primates, but the chitinous exoskeletons of arthropods have long been considered to be indigestible by the digestive enzymes of most mammals. However, recently mice and insectivorous bats were found to produce the enzyme acidic mammalian chitinase (AMCase) to digest insect exoskeletons. Here, we report on the gene CHIA and its paralogs, which encode AMCase, in a comparative sample of nonhuman primates. Our results show that early primates likely had three CHIA genes, suggesting that insects were an important component of the ancestral primate diet. With some exceptions, most extant primate species retain only one functional CHIA paralog. The exceptions include two colobine species, in which all CHIA genes have premature stop codons, and several New World monkey species that retain two functional genes. The most insectivorous species in our sample also have the largest number of functional CHIA genes. Tupaia chinensis and Otolemur garnettii retain three functional CHIA paralogs, whereas Tarsius syrichta has a total of five, two of which may be duplications specific to the tarsier lineage. Selection analyses indicate that CHIA genes are under more intense selection in species with higher insect consumption, as well as in smaller-bodied species (<500 g), providing molecular support for Kay’s Threshold, a well-established component of primatological theory which proposes that only small primates can be primarily insectivorous. These findings suggest that primates, like mice and insectivorous bats, may use the enzyme AMCase to digest the chitin in insect exoskeletons, providing potentially significant nutritional benefits.


Extras: Filter Print Post Top
Offlinelight4x
Stranger

Registered: 01/07/19
Posts: 110
Last seen: 1 year, 9 months
Re: How to grow and consume Lions Mane mycelium? [Re: skywhisp]
    #26538477 - 03/16/20 12:08 PM (3 years, 10 months ago)

Nice find on the chitin digestion.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: Mushroom-Hut Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates


Similar ThreadsPosterViewsRepliesLast post
* Question about lions mane extract HELP *DELETED* MycoMykey 678 4 05/05/21 03:19 PM
by Forrester
* Lions Mane and Shiitake plates Psilocybeingzz 2,955 2 09/29/04 01:05 PM
by Psilocybeingzz
* After how long should I see lions mane growth on agar from old spores? ninja cat 09 668 8 05/10/21 02:58 PM
by QM33
* Lion's mane from a woodland mycobag MilletV 4,330 16 03/16/04 03:18 PM
by CaptainFuture
* Lion's mane wedge charlieXxXxX 3,999 18 05/07/04 12:51 PM
by Speeker
* Lion's Mein LOBO 3,576 8 04/16/23 05:02 AM
by rxb
* Want 2 grow gourmet! nononsense 2,006 2 06/11/01 06:33 AM
by egghead
* Need suggestions on which edible will grow sporgasm 1,923 11 06/21/04 10:28 PM
by sporgasm

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
4,478 topic views. 1 members, 9 guests and 3 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.008 seconds on 15 queries.