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Offlinepsycho_nauticus
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Bulk substrate not colonizing
    #26491199 - 02/18/20 05:58 AM (3 years, 11 months ago)

Spawned my shoebox two days ago. I’m not seeing the slightest bit of any sign of starting colonization... I didn’t use a liner, so I’m able to see it from the sides...


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Offlineiwh678
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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26491202 - 02/18/20 06:02 AM (3 years, 11 months ago)

Be little more patient. Two days is not a lot of time.


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: iwh678]
    #26491249 - 02/18/20 06:57 AM (3 years, 11 months ago)

Okay, I was anticipating a little spreading somewhat into the coir and substrate. Not unreasonable unrealistic amount of colonization, I was thinking something would at least start now 2 days in. About how many days in should I be worried if I still don’t see any signs of colonization in the bulk substrate?


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OfflineOmbisha
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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26491448 - 02/18/20 09:41 AM (3 years, 11 months ago)

Quote:

psycho_nauticus said:
About how many days in should I be worried if I still don’t see any signs of colonization in the bulk substrate?




You should see growth sooner than that, but let's say a week. Could it perhaps be that your bulk sub was not field capacity? If the substrate is too wet, to growth will stall.

Do you see any exposed grains? As in not covered with the substrate? You should see growth around such grains in about 36 hours, but don't go digging in your substrate. Be patient, good luck!


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: Ombisha]
    #26491576 - 02/18/20 10:59 AM (3 years, 11 months ago)

If I feel like my sub may be too wet is there anything I can do? I did the field capacity test whenever I prepped it. Nothing came out at first, but when I squeezed really hard a light stream trickled and dripped. Now I’m thinking it’s too wet because I’m reading only a few drops should of come out. I had a little more than a few drops. I used Damions5050 coir tek. Is there anything I can do, or I just wasted and fucked my tub up?


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26491588 - 02/18/20 11:09 AM (3 years, 11 months ago)

Calm down man you’re good lol.

It’s like when you shake the jars, it won’t really recover and start moving again for a few days. 5 days is when I usually see circles of myc forming on the bottom and sides.

It sounds like your sub is right where it should be with water content. I made my first few to where when I squeezed really hard only a couple drops came out and it was waaayy too dry.


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Edited by A.k.a (02/18/20 11:10 AM)


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26491909 - 02/18/20 02:51 PM (3 years, 11 months ago)

Okay got it.

I was starting to think maybe I really did have bad spawn and wasn't colonized correctly.

We were talking about how yours get done in 5 days, and with the amount of spawn I used I should also. I'm on the third day and nothing is really happening or moving, so I was concerned...


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OfflineSYF8
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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26491936 - 02/18/20 03:09 PM (3 years, 11 months ago)

Be patient and don’t get concerned unless it starts to smell bad or the spawn color changes to black


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OnlineA.k.aM
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Re: Bulk substrate not colonizing [Re: SYF8]
    #26491956 - 02/18/20 03:23 PM (3 years, 11 months ago)

I’ve had some lately run it in five days but 7-8 is more average.

Are the other jars looking any different or just a little thicker?


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26491975 - 02/18/20 03:31 PM (3 years, 11 months ago)

Just thicker like this, here it is: (I can't tell anymore...)



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Offlineiwh678
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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26491999 - 02/18/20 03:49 PM (3 years, 11 months ago)

That looks bacterial, but not so much it wouldn't colonize.


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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26492001 - 02/18/20 03:51 PM (3 years, 11 months ago)

This is 2-1/5 days after spawn with 4.5 jars of rye and damion5050 substrate in a 54 qt unmodded straight to fruiting.  I had quite a lot of exposed grains. What temperature is your grow in?  Mine was around 76 most of the time.  That was my first mono tub and I was amazed how fast it colonized.  Pins in 10 days and lots of fruits a week later.



a few days ago:



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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: Odo]
    #26492008 - 02/18/20 03:55 PM (3 years, 11 months ago)

2 1/2 days??? I'm feeling even more that something is up with mine then...

My room temperature is in the low 70's: 70-75


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: iwh678]
    #26492010 - 02/18/20 03:58 PM (3 years, 11 months ago)

What does this mean for me then? Lots of other people in my other thread said they looked good and I'm good to go and to spawn it...

Will my bulk substrate not colonize then and just contam straight out?


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26492033 - 02/18/20 04:21 PM (3 years, 11 months ago)

It doesn't mean anything about yours. Again just be patient, you have no reason to worry at this point.

That spawn isn't terrible. I have spawned far worse without contams.


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: iwh678]
    #26492862 - 02/19/20 05:58 AM (3 years, 11 months ago)

I’m trying to be patient, but at the same time aware of any problems. It doesn’t look like my grain is bouncing back from spawning. Also, I noticed that it looks like only the tips of the individual grains are colonized, and the rest of the grain itself is bare. Is this the reason why my jars looked the way they did? I thought maybe it looked like that because I’ve heard millet kind of looks funny when colonizing. I’m also thinking this has something to do with the reason why my substrate isn’t colonizing...? I’m not expecting it to be done or anything, but I do think that I should of been seeing partly or even the start of colonization 4 days in. Nothing is taking over the substrate or coir, and from what I’ve read when that starts it just eats through the coir rapidly colonizing. I see no such thing. It’s just a bunch of millet grains with white tips still sitting in my bulk...


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26493007 - 02/19/20 08:18 AM (3 years, 11 months ago)

You can’t compare it’s speed until you’ve run it before and know how it is.

These two are both the same age.



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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26493198 - 02/19/20 10:49 AM (3 years, 11 months ago)

Yeah for sure, I’m going to keep waiting. I’ve just been reading all different types of stuff, and if the grain colonization affected it in any way. That’s what I noticed the last few days and have been reading up on. Hopefully my grain and spawn did colonize correctly! But everyone seemed to concur that I was good from my pictures, and you guys got all the experience!!! How does the description of the grains sound to you?


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Edited by psycho_nauticus (02/19/20 10:58 AM)


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26493587 - 02/19/20 03:12 PM (3 years, 11 months ago)

Came home from work, and shoebox had an awful smell to it. Had to throw it out. So now it's definitely a fail. My grains never recovered after the spawning and just sat in the bulk substrate for 4 days never colonizing any of it, not a tiny sliver. I'm not understanding wtf happened....

Hopefully my agar comes through, that's all I have now...


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Edited by psycho_nauticus (02/19/20 03:18 PM)


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26493628 - 02/19/20 03:47 PM (3 years, 11 months ago)

What did it smell like?

No visible contamination though?

I’ve had a bunch of weird smelling tubs that did ok, but I wouldn’t describe it as awful. More like spicy fruit maybe slightly rotting


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26493694 - 02/19/20 04:34 PM (3 years, 11 months ago)

That's the best way to describe it, had a spice like smell to it, and I could tell it was off. Maybe I shouldn't say awful, but it was definitely off. It was just sitting in my sub for 4 days nothing happening. No visible contamination. Everything that I've seen, researched, and looked at says as soon as there is a bad smell if it happens then toss it. This has taken so much out of me... The investment of time and energy and then fail. Very sad and disappointed. Maybe I thought millet just looks weird when it colonizes, but really it never really colonized... The grains were sitting in my bulk, but you could see they were all still yellow millet color, and only the tips of the grain had some white, only the tips. I keep looking back at my pictures and the two jars I still have, they still look like that. For some reason the mycelium isn't encapsulating every grain, I think that's why it looks like that in my jars, and that's why it never started colonizing my sub (this is just my educated guess). My other two jars look the same but thicker, but no additional growth or colonization has happened, and still some grains seem exposed, and not covered and half covered, etc. I'm not sure as to why this happened or why they're like that, I did everything sterile and inoculated in my SAB. I feel like these other two jars are going to do the same exact thing because some grains don't seem colonized, and only half covered etc. Again, the mycelium isn't encapsulating every grain, and I don't know why. I'm just in a down and funky mood right now, this sucks.


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26493842 - 02/19/20 06:06 PM (3 years, 11 months ago)

Man you should go get it out of the trash until you see mold. I had three shoeboxes early on that smelled so strong when I opened them I was asking if different varieties smelled different lol. And got good hauls off all of them.

No reason to tap out before you need to, worst case you learn how bad tubs look and smell best case you get an ounce of shrooms along with the experience.


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26493845 - 02/19/20 06:09 PM (3 years, 11 months ago)

I personally think you didnt wait long enough. If nothing visible is wrong then I keep waiting. 4 days is such a small amount of time to wait after spawn I would have waited atleast a week.


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: WyoMX]
    #26493885 - 02/19/20 06:34 PM (3 years, 11 months ago)

I wasn't expecting to have fruits or pinning or something in 4 days. I was expecting to see at least a sliver of some type of colonization of the sub. Even just a little hair of coir or something, or even any sign of the grains even slightly bouncing back after the spawn, but nothing. Then a smell came, so I was just doing what is recommended when that happens.


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26493893 - 02/19/20 06:36 PM (3 years, 11 months ago)

I can, it's still here, I snapped the lid on and boxed it up neatly in a box to throw out. If I do that just to wait around and see mold then won't I be spreading more contaminants and mold spores in my place for my next try or agar, etc.?


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26494070 - 02/19/20 08:44 PM (3 years, 11 months ago)

You should be okay. My shoe boxes and mons also took around 8 days for signs of growth. The biggest lesson I had to learn when starting is keep your mind off of it, because you will think of all kinds of little things to worry you, you think it looks too dry, too wet, then you start fucking with it and you then you wish you wouldn't have touched it haha. If your spawn was clean and you were good in your cleansing/sanitation tech, you will be all good.


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26494092 - 02/19/20 09:12 PM (3 years, 11 months ago)

THere are more mold spores in your house that a shoebox can create.

Don't toss a bin until you know it is trash, also I thought your original jar might have had a few spots of Bactiera or yeast on a few grains, but that isnt a good reason to toss it.

Then instead of trashing it put it in a shady spot in the back yard you can keep moist and Bam, outdoor sub!


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Re: Bulk substrate not colonizing [Re: Sockadin]
    #26494114 - 02/19/20 09:30 PM (3 years, 11 months ago)

I usually close my tubs up after spawning for 10/11 days to let them colonize. It kills me waiting that long but usually when I check on that 11th day I have a good covering of mycelium and usually a fair scattering of knots. patience is a virtue even if it sucks! I've learned to tuck my spawn jars away for a couple of weeks at this point too so I don't poke at them. I say give it some time and best of luck!


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Re: Bulk substrate not colonizing [Re: PupMarvel]
    #26494145 - 02/19/20 09:54 PM (3 years, 11 months ago)

I wouldn’t worry about mold spores, if a patch of trich shows up you’ll have plenty of time to ditch it before it gets bad.

You gotta get that PMA, to quote the magnificent bad brains.

Even if it doesn’t work out you can still learn from it. I’ve got a few totally fucked spawn jars they’ve been sitting around for a couple weeks just to try and get an idea of what contamination does.


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Edited by A.k.a (02/19/20 09:54 PM)


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26494367 - 02/20/20 03:27 AM (3 years, 11 months ago)

PMA? What do you mean by that?

Took it back out.


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: Sockadin]
    #26494373 - 02/20/20 03:36 AM (3 years, 11 months ago)

Yeast? I did not see any yeast build up on anything when I look at the pictures of my jars, nor do I remember being told that.

Also, my temps are between 70 and 74 for this stage, that sound correct?


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26494908 - 02/20/20 10:48 AM (3 years, 11 months ago)

Hell yeah! Hopefully it redeems itself.

Lol pma is an old punk thing from the 80s, positive mental attitude.


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26494936 - 02/20/20 11:02 AM (3 years, 11 months ago)

Still looks the same going on day 5... Grains are sitting in there all yellow with only a little white on the tips.

Sitting in a condensated tub, lid cracked for FAE, smells a little stronger - I’m sure it’s because it’s just sitting in condensation and wetness still no sign of anything. Will wait through the weekend though, that’s three more days...


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26494944 - 02/20/20 11:05 AM (3 years, 11 months ago)

Are you talking about grains on the side of the tub or surface?  A lot of times I’ll get clumps of seeds against the side of the shoebox and they don’t fuzz up again it just runs through the coir.

If you pick it up and look at the bottom can you see myc spreading at all? That’s usually where it’s easiest to notice at first.

Also if you tossed it around when you threw it out before it might be stunned for a day or so.


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Edited by A.k.a (02/20/20 11:06 AM)


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26494989 - 02/20/20 11:32 AM (3 years, 11 months ago)

I didn't toss it around or anything, I just snapped the lid back on and gently put it in another box so I could throw the whole box away with it inside. Basically it was the same as just sitting as it is now but inside of a box. Nothing got tossed, thrown, turned, etc.

I'm talking about everywhere, and all over, sides, surface, etc.

Nope, nada, nothing. Just yellow millet grains with white on the tips. Nothing spreading or doing anything.


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26495001 - 02/20/20 11:43 AM (3 years, 11 months ago)

Mycology is not a hobby for the impatient. Just leave it sitting for a few days and let it do its thing. Maybe you are disturbing the micro-climate.


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Re: Bulk substrate not colonizing [Re: Ombisha]
    #26495043 - 02/20/20 12:06 PM (3 years, 11 months ago)

It's been sitting there 5 days. I've only touched the tub twice, whenever I put it in the box to throw away and when AKA just had me check the bottom now. I don't want to mess with it at all as you point out, and haven't been messing with it.

Thank you for the noob advice smh. I've never heard that before smh...

I'm a noob too, so a noob recognizes a noob. Especially with a comment like that that I haven't heard before


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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26495117 - 02/20/20 12:41 PM (3 years, 11 months ago)

Pictures would really really help us gage if there's something wrong with it. Yellow doesnt sound good but not always bad.


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Re: Bulk substrate not colonizing [Re: WyoMX]
    #26495143 - 02/20/20 12:57 PM (3 years, 11 months ago)

Code:

I mean yellow as in the color of the grains, or the color of millet. Millet I guess is like a pale yellowish color. And only the tips of the grains are a little white.

I was taking an educated guess as to why my jars also looked like that. For some reason the mycelium doesn't appear to be encapsulating the whole grain, leaving most of it bare, except a little at the tips, just like now. I am presuming that's what happened in my jars also, but I just chalked it up to that must be the way millet looks when it colonizes. Not every grain was completely covered, they were like half covered with half still exposed, some were totally covered, and some not at all, but it was very thick and fuzzy strong mycelium, and it did seem to appear that it was fully colonized. I had posted pictures of my jars before spawning and people said I was definitely good. I'll get some pictures up for you tonight of my shoebox.

In the meantime, here are the jars that I spawned to this shoebox.



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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26495237 - 02/20/20 01:45 PM (3 years, 11 months ago)

Well what are you expecting to hear? There are no signs other than "no growth". Give it some time, sometimes it takes just that. Either you can overthink it and wondering what's wrong or you can just be patient. The result will be the same. :shrug:


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Re: Bulk substrate not colonizing [Re: Ombisha]
    #26495838 - 02/20/20 06:39 PM (3 years, 11 months ago)

Well hopefully it does something soon. That’s just how the millet looks, even a couple TCs said those jars were ready to go.

Im pretty interested what’s going on, maybe you just got the slowest culture ever. I’ve never spawned something and had nothing happen, even if it was garbage spawn that went green in a week the myc still grew.

I’m assuming you didn’t mix the spawn when the coir was like 140 degrees.

Here’s a jar I’m about to spawn, millet is the only thing visible.


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LAGM2020


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: A.k.a]
    #26496497 - 02/21/20 06:29 AM (3 years, 11 months ago)

No way, I waited until it cooled down. To be more specific, I had prepped it around midnight and decided to spawn the next morning since it was getting so late. So it was like 8 hours in between and had definitely cooled because I felt it when I handled it and tested it for field capacity also.

Slowest culture ever could be right... maybe... My agar has still done nothing. But it didn't take a long time for germination in the jar, only several days. Now the actual colonization of the jar took like 2 weeks, and took 10 days post shake. I've ruled out that it can't be my sterilization or technique when inoculating because no contaminants have showed up on my agar plates either.

Yeah you're jar looks the same as my jar. Thanks for the comparison. How are you spawning that one?


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Offlinepsycho_nauticus
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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #26498041 - 02/22/20 06:56 AM (3 years, 11 months ago)

Tossed tub out today, gone for good this time. Not one single hair of coir or sliver of substrate began to colonize after one week. Grains just sat there. Not sure what went wrong. If jars were bacterial I was still expecting something out of it, some small yield, but it didn't even attempt to make it that far. Not sure what the fuck happened. I want to see the agar results, if it ever freaking happens...


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OfflineHatefulHollow
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Re: Bulk substrate not colonizing [Re: psycho_nauticus]
    #27276686 - 03/31/21 05:39 AM (2 years, 9 months ago)

I've run into the exact same situation. Did you eventually figure out what was wrong with your sub?


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