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Offlineinsanegrain
Stranger
Registered: 11/13/19
Posts: 12
Last seen: 2 years, 10 months
Contaminated LM Agar Plate
    #26490157 - 02/17/20 02:38 PM (3 years, 11 months ago)

Hello everyone. I recently got some agar wedges that I inoculated in some plates. The reishi looks nice and healthy, but the lions mane is contaminated. I was wondering if it was worth trying to save it or purchase more lions mane wedges and start over. I tried to cut a smaller piece, but the whole wedge ended up falling in. Looks like there are two different types of mycelium in the plate. One is more whispy looking and the other is more dense and growing more like the reishi.

Here is a link with the pictures of the contaminated plate.





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Invisiblerm1024
Familiar Stranger
Registered: 11/05/18
Posts: 62
Re: Contaminated LM Agar Plate [Re: insanegrain]
    #26490257 - 02/17/20 03:13 PM (3 years, 11 months ago)

Ive got some beautiful LM plates but its quite hard to know where to transfer from as it looks very different than anything ive worked with before.  Will be following along to see if i can get any insight!


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OfflineForresterM
aspiring sociopath
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Registered: 02/05/13
Posts: 9,351
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Last seen: 24 days, 18 hours
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Re: Contaminated LM Agar Plate [Re: rm1024]
    #26491292 - 02/18/20 07:34 AM (3 years, 11 months ago)

That green mold in the first pic has already dumped spores, it's possible to clean up but I wouldn't even attempt it.

If you're desperate you can, lion's mane grows really fast and you should be able to grab something before the mold you also transferred sporulates, but it may take a lot of transfers.


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlineinsanegrain
Stranger
Registered: 11/13/19
Posts: 12
Last seen: 2 years, 10 months
Re: Contaminated LM Agar Plate [Re: Forrester]
    #26491996 - 02/18/20 03:48 PM (3 years, 11 months ago)

Thanks for the replies. I figured it would be best to start with a different sample. For reference, how should the mycelium look for lions mane? The top part looks more wispy like I've read it would look like, and the lower part looks more like typical mycelium I have seen.


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OfflineForresterM
aspiring sociopath
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Registered: 02/05/13
Posts: 9,351
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Last seen: 24 days, 18 hours
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Re: Contaminated LM Agar Plate [Re: insanegrain]
    #26492931 - 02/19/20 07:24 AM (3 years, 11 months ago)

it does thicken up after a bit, but not too much unless it's turning to fruit


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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