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Svenny
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Is this lions mane spawn too late for grain to grain transfer?
#26489843 - 02/17/20 11:33 AM (3 years, 11 months ago) |
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I let this Grain spawn of lion's mane go too long, and was wondering if I could still use it for grain to grain transfers? It's been 2 weeks, and I used liquid culture to inoculate it. Anything suggestions would be awesome, thanks!
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iwh678
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: Svenny]
#26489848 - 02/17/20 11:39 AM (3 years, 11 months ago) |
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Waiting a week or two isn't usually an issue, but that spawn is not clean. It looks like bacterial and maybe a yeast contamination as well.
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Svenny
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: iwh678]
#26490139 - 02/17/20 02:32 PM (3 years, 11 months ago) |
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Could it look weird because it is starting to fruit? The pictures aren't the best. It was a nice light coating of wispy mycelium then I left it too long and now it looks like it might me starting to fruit. I know lions mane is notorious for that.
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rm1024
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: Svenny]
#26490286 - 02/17/20 03:24 PM (3 years, 11 months ago) |
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it looks different than maybe cube would but im curious as to what gives it away that its not right? im only just starting looking into and working with LM and would appreciate any insight.
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Svenny
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: rm1024]
#26490898 - 02/17/20 10:31 PM (3 years, 11 months ago) |
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Same here. I could see if you're comparing it too cubensies, then it would look odd. I seen other similar pictures of lions mane that went to long and it looked like this. I used the same filter lids I use for oyster spawn. Maybe there was too much oxygen in the jars, I know you should limit it for lions mane when its colonizing otherwise it tends to fruit early.
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seagu

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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: Svenny]
#26491208 - 02/18/20 06:11 AM (3 years, 11 months ago) |
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I am not a Lion's Mane expert by any stretch. But the pics look all white.....
-------------------- Plan to win or you are planning for failure. Don't let anyone tell you you can't do it. Just figure out the solution. Even if that means banging your head on a wall until the solution oozes out of you.
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iwh678
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: seagu]
#26491226 - 02/18/20 06:37 AM (3 years, 11 months ago) |
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No I'm not comparing to cube myc. I'm comparing to past Hericium grows I've done. That's not clean spawn.
"All white" is not the only qualifier for clean spawn
Edited by iwh678 (02/18/20 08:58 AM)
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Forrester
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: iwh678]
#26491281 - 02/18/20 07:28 AM (3 years, 11 months ago) |
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Any of us could be wrong, it's just a pic, but I'm gonna chime in and say it doesn't look contaminated to me, just a bunch of fruit in there.
Anyway I wouldn't g2g it anyway so a bit irrelevant.
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seagu

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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: Forrester]
#26491398 - 02/18/20 09:16 AM (3 years, 11 months ago) |
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While I agree all white is not the definitive qualifier, as Forrester points out they are just pics and we all could be wrong because of that, and so I made a play on words of it looks - all white, all right.
LM myc does grow different than Oysters and that very well could be LM myc starting to fruit. But again I am not a LM expert.
-------------------- Plan to win or you are planning for failure. Don't let anyone tell you you can't do it. Just figure out the solution. Even if that means banging your head on a wall until the solution oozes out of you.
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Svenny
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: seagu]
#26492153 - 02/18/20 05:23 PM (3 years, 11 months ago) |
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I see the hair like icicles starting to form. You are right though it's probably started to fruit before it could fully colonize the grain, don't want to risk contamination from the uncolonized grain spawn in the transfer jars. Maybe I'll drop a few colonized grains on agar to save it for next time.
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seagu

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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: Svenny]
#26492411 - 02/18/20 07:59 PM (3 years, 11 months ago) |
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LM pretty much always starts to fruit before it looks like it is fully colonized if you compare it to oysters in myc look on full colonization though. LM will be fully colonized on the grain and not really look thick white because LM myc is very thin and wispy. With LM you don't want it completely thick white all over the grains before you fruit like with Oysters and such.
-------------------- Plan to win or you are planning for failure. Don't let anyone tell you you can't do it. Just figure out the solution. Even if that means banging your head on a wall until the solution oozes out of you.
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Smoothcat
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: seagu]
#26492514 - 02/18/20 09:13 PM (3 years, 11 months ago) |
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Lions mane is completely different to cube myc, I took this off some wood spawn to agar and thought yeah it’s fucked defo contam and I’m gonna have to toss this plate

Then when I held it up to the light I saw very thin, what looks to me from other pics I’ve seen, lions mane myc that you could only really see when you hold it up to the light.

Now I’ve just done a transfer from it because there definitely is something nasty on there. Maybe I’ve just transferred Mold but apparently lions mane myc does this, starts really thin and almost invisible then suddenly it just goes mad and wants to fruit...we shall see.
I think your grain jars look ok, ready to fruit
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seagu

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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: Smoothcat]
#26492872 - 02/19/20 06:13 AM (3 years, 11 months ago) |
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Yup. When I first did LM, which was in bags, it was sitting there forever and I thought it wasn't doing anything.. because I didn't know about the thin and wispy part and look. I had always grown Oysters and didn't even occur to me to check to see if LM myc or whatever would have a different look. Then after a long time it started to thicken up and start to get thick white like I was used to with the Oysters. Little did I know yet that it was trying to fruit. So, I just waited for it to thicken up, thinking it would be a thick white like Oysters and then set to fruiting, wait a week to see pins... wrong answer... It thickened up alright and started fruiting like mad all over inside the bag.
-------------------- Plan to win or you are planning for failure. Don't let anyone tell you you can't do it. Just figure out the solution. Even if that means banging your head on a wall until the solution oozes out of you.
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Svenny
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: seagu]
#26496148 - 02/20/20 09:41 PM (3 years, 11 months ago) |
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It's nice to hear all the responses. Yep that's exactly what happened oh, I left it sit too long. It definitely grows a lot different then oyster mycelium does. Next time I guess.
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DigitalRhizae
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Re: Is this lions mane spawn too late for grain to grain transfer? [Re: Svenny]
#26506763 - 02/27/20 03:59 PM (3 years, 10 months ago) |
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The condensation on the top of the jar may be bacteria, but it may just be condensation. Have you shaken the jars at all? If you shook them and they recovered it's less likely to be bacterial contamination. If they smell bad that would be a good indication. I just recently transferred some agar of a wild Hericium species I cloned and the jars started to smell of sulfur. I shook them and shortly after what looked like condensation formed on the top part of the glass, the smell increased and the mycelium never recovered. Either my plates were dirty or I wasn't sterile enough when transferring. Just something to consider. But as others said lions mane will look like that when over-colonized.
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