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OfflineGnirut
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Registered: 02/16/20
Posts: 2
Last seen: 3 years, 11 months
Contamination or bruising in rye grain jar?
    #26488848 - 02/16/20 06:19 PM (3 years, 11 months ago)

Hi!

I am currently colonizing some rye grains (I did a G2G transfer from a healthy jar).
One of the jars is well colonized but there are some blue spots that are worrying me, and I don't know if it is bruising or trichoderma.




Do you know what it is?
If you are not sure (I did my best to have good pictures but we don't see as well as in real life), is there some test that I can do? (For example, shaking the jar and see if the blue spots develop or not?)

I am using a tight polyfill filter for FAE. To protect my jars from light I put them under a sweater, maybe this causes a lack of FAE which cause those blue spots?

Also, since the blue spots are very small, would it safe to spawn it in CVG?

Thanks!


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Offlineiwh678
Budget Mycologist
Male


Registered: 01/22/14
Posts: 396
Loc: USA
Last seen: 1 year, 6 months
Re: Contamination or bruising in rye grain jar? [Re: Gnirut]
    #26488936 - 02/16/20 07:11 PM (3 years, 11 months ago)

Its really hard to tell. I don't think it looks good, but it way to early to say. You'll definitely know in couple days.


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OfflineGnirut
Stranger
Registered: 02/16/20
Posts: 2
Last seen: 3 years, 11 months
Re: Contamination or bruising in rye grain jar? [Re: iwh678]
    #26490675 - 02/17/20 07:09 PM (3 years, 11 months ago)

Indeed, waiting a day showed that it was a contamination.

About my question about FAE: can a lack of FAE cause a contamination (for example because the mycelium can't grow "fast enough" and "fight" the contaminent?), and do you know if my "sweater tek" for hiding light reduces FAE?

Thanks!


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OfflineMrBovineJoni
Glip-Glop


Registered: 11/28/19
Posts: 264
Last seen: 13 days, 14 hours
Re: Contamination or bruising in rye grain jar? [Re: Gnirut]
    #26491076 - 02/18/20 02:39 AM (3 years, 11 months ago)

Quote:

Gnirut said:
Indeed, waiting a day showed that it was a contamination.

About my question about FAE: can a lack of FAE cause a contamination (for example because the mycelium can't grow "fast enough" and "fight" the contaminent?), and do you know if my "sweater tek" for hiding light reduces FAE?

Thanks!




The lack of FAE pretty much under any conditions is not the main cause of  contamination on its own. However, under certain conditions lack of FAE can help contams grow - for example cobweb mold on top of spawn/substrate in monotubs.

At the spawn colonisation stage (e.g. jar of grain) what you have is not really FAE (fresh air exchange) but filtered GE (gas exchange - if done right it is almost always filtered) via SFDs or tyvek or polyfil fibres. They are not the same concept. FAE only becomes very important at pinning and fruiting stages of the growth process (which are not sterile anymore) - at which point the spawn should already be colonised, contaminant free and able to fight off contaminants to a certain degree.


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