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OfflineBryan1206
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Registered: 11/05/19
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Colonized grain ready for bulk?
    #26488028 - 02/16/20 08:56 AM (3 years, 11 months ago)

How does this grain look? It’s from a GT spore syringe, I hope it’s not bacterial because I feel like the growth is not that even, or maybe it just needs more time.

I also have agar transfers going and I was wondering if I would be able to effectively cut open one of these grain bags and put some agar slices in there and tape it back up? I don’t have a pc or flow


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OfflineMycoactive
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Registered: 11/20/19
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Re: Colonized grain ready for bulk? [Re: Bryan1206]
    #26488039 - 02/16/20 09:03 AM (3 years, 11 months ago)

They look good to me. I would not add agar slices. That will simply increase your risk of contamination, and if the bags are already contaminated (which, again, they don't appear to be), then addition of agar slices is not going to help. How did you prep the grains without a PC?


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Offlineendlessnessagain
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Registered: 01/25/20
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Re: Colonized grain ready for bulk? [Re: Bryan1206]
    #26488042 - 02/16/20 09:04 AM (3 years, 11 months ago)

They look good to me..

Why not make a SAB for the agar?

Also why not do the agar once you feel they are ready for mixing with bulk, so you dont have risks of opening up and closing it again ?


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OfflineBryan1206
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Re: Colonized grain ready for bulk? [Re: endlessnessagain]
    #26488047 - 02/16/20 09:09 AM (3 years, 11 months ago)

Oops sorry I explained that wrong, what I meant was open up a new bag of grain that’s un colonized. And I use a cardboard box as a SAB, just make sure to thoroughly clean and disinfect the area and have a air purifier running for a couple hours before hand


Edited by Bryan1206 (02/16/20 09:11 AM)


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OfflineSockadin
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Re: Colonized grain ready for bulk? [Re: Bryan1206]
    #26488419 - 02/16/20 01:29 PM (3 years, 11 months ago)

Do your parents ask what's up with the cardboard box and air purifier?


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