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OfflineRavitch
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Registered: 02/14/20
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Alcohol in Substrate Bag
    #26484738 - 02/14/20 05:55 AM (3 years, 11 months ago)

Alcohol in Substrate Bag


Before inoculating a filter bag, a small amount of alcohol got in through the filter patch. (As I was spraying the bag down, the top of the spray bottle came loose and alcohol spilled in).

Have I completely killed the bag and any spores I inject, or is there a way to salvage it?


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OfflineMushkingMulah360
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Re: Alcohol in Substrate Bag [Re: Ravitch]
    #26484744 - 02/14/20 05:59 AM (3 years, 11 months ago)

Should be ok how much really go into the bag though? Just keep an eye on it nothing you can really do at this point! Next time spray a PT and wipe the bag down lol.


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OfflineRavitch
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Re: Alcohol in Substrate Bag [Re: MushkingMulah360]
    #26484773 - 02/14/20 06:21 AM (3 years, 11 months ago)

Good idea for next time lol, I would say about 1/8 to 1/4 of the patch got visibly wet, I moved the bottle out of the way ASAP.


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Invisiblefilthyknees
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Re: Alcohol in Substrate Bag [Re: Ravitch]
    #26484776 - 02/14/20 06:23 AM (3 years, 11 months ago)

No body knows dude. It's probably fine - I don't think it went THROUGH the patch.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
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Offlineiwh678
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Re: Alcohol in Substrate Bag [Re: filthyknees]
    #26484838 - 02/14/20 07:07 AM (3 years, 11 months ago)

Yeah I'd say its fine then.


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InvisibleSmartattack
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Re: Alcohol in Substrate Bag [Re: iwh678]
    #26484848 - 02/14/20 07:15 AM (3 years, 11 months ago)

It's fine. I've sprayed my tubs with iso multiple times. They don't miss a beat.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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OfflineRavitch
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Re: Alcohol in Substrate Bag [Re: filthyknees]
    #26484856 - 02/14/20 07:22 AM (3 years, 11 months ago)

A little bit did get through, I saw it run down the bag. But everyone seems to think it’s not too big of a deal overall so that’s really good news.


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OfflineBLINKfan420
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Re: Alcohol in Substrate Bag [Re: Ravitch] * 1
    #26484936 - 02/14/20 08:21 AM (3 years, 11 months ago)

I'm gonna play devil's advocate and mention that alcohol is not sterile.  This is why people get contams when going from flame sterilizing then wiping with iso. 

I'd hope you're bag is good too but the more you know. 🌠

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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Offlineiwh678
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Re: Alcohol in Substrate Bag [Re: BLINKfan420]
    #26484940 - 02/14/20 08:24 AM (3 years, 11 months ago)

I wouldn't say its the alcohol that isn't sterile but rather the paper towel or what ever you soak with alcohol.


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OfflineRavitch
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Re: Alcohol in Substrate Bag [Re: iwh678]
    #26485137 - 02/14/20 10:22 AM (3 years, 11 months ago)

If that’s the case, then alcohol falling into the bag directly from the bottle should not introduce contaminants?


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InvisibleSmartattack
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Re: Alcohol in Substrate Bag [Re: Ravitch]
    #26485146 - 02/14/20 10:28 AM (3 years, 11 months ago)

Sure it could but pretty unlikely.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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