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OfflinePTreeDish
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Registered: 04/22/18
Posts: 353
Last seen: 3 months, 15 days
Lessons from Pouring 50 Agar Plates
    #26484413 - 02/13/20 09:31 PM (3 years, 11 months ago)

Poured the first 50 plates in the new clean room. Made some mistakes and learned a few things.

* Boil over is real - mine wasn't too bad but could do a better job next time. Wool/cotton plug maybe?
* Poured while agar was too hot in fear it'd cool too quickly. Smaller batches and/or a hot bath or hot plate to hold the temp could help.
* Avoid EZ Bioresearch Sterile 100 mm X 15 mm dishes as the over-sized lids make it difficult to pick up a stack with one hand for sterile pours.
* Cut the 1" parafilm into .5" pieces by 2-3" long pieces. I way over-wrapped my dishes, possibly preventing good air exchange from pure ignorance.
* Condensation in the plates is an issue. Ensured plates were stacked when poured. Tried to put hot media bottles on top but it did nothing. Allowing the plates to cool in front of the fan overnight helped.
* Day after pour, decided to store all uncultured dishes upside down per several forum posts I read.










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