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PTreeDish


Registered: 04/22/18
Posts: 353
Last seen: 3 months, 15 days
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My Sterile Room
#26484377 - 02/13/20 09:05 PM (3 years, 11 months ago) |
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I thought I'd share the sterile room I built in my attic. It's made from 3/4" PVC, 6 mil plastic and duct tape.
As far as locations go, it's probably one of the worst. My attic is full of white rot mold from old roof leaks and there are skylights everywhere that act serve as as ventilation. But this was the only spot the spouse would concede.
There is a small initial still air room that houses the entrance to the main room. The door is a Zip Wall.
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Shroomwomb
Chad Thundercock



Registered: 04/22/18
Posts: 63
Loc: Seychelles
Last seen: 3 years, 8 months
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Re: My Sterile Room [Re: PTreeDish]
#26484395 - 02/13/20 09:14 PM (3 years, 11 months ago) |
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Very cool! I am not familiar, what is the advantage of having a sterile room vs. just building a flow hood?
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 6 hours, 19 minutes
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Re: My Sterile Room [Re: PTreeDish] 1
#26484414 - 02/13/20 09:31 PM (3 years, 11 months ago) |
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If you have a working flowhood I don't think any of that is really going to improve anything. It's kind of ironic too that you're building a sort of low contam tent but then you're putting the most contaminant ridden thing in the house inside of it, your own body. The most effective barrier is one that is placed between you and the stuff you're working on.
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PTreeDish


Registered: 04/22/18
Posts: 353
Last seen: 3 months, 15 days
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Re: My Sterile Room [Re: Kizzle]
#26484439 - 02/13/20 10:01 PM (3 years, 11 months ago) |
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Stamets book recommended a proper clean room having up to 4 entrances before entering the final room. So in my model, I created a still air entrance room that is about 4x4 that houses the zip wall. So it's basically one additional place to minimize introducing dirty attic air.
When entering the first entrance, there is 70% IPA and gloves to wipe myself down and everything around me down. When I unzip the main entrance, positive pressure from the hood running inside minimizes the introduction of additional contams upon entry.
I've also taken measures to clean and sterilize the pathway I take outside of the tent to get to it. Finally, I have some degree of climate control in this room and have the flexibility of tapping an outside air source and directing it only into this space when needed.
Is any of this necessary? I don't know but I could not, in good conscience, simply work in the open air of my attic.
Edited by PTreeDish (02/13/20 10:03 PM)
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Bph
Stranger



Registered: 10/11/18
Posts: 1,466
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Re: My Sterile Room [Re: PTreeDish] 1
#26484655 - 02/14/20 03:56 AM (3 years, 11 months ago) |
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It's cool as shit and if you got the time and money why not. But bro you don't need that for growing some shrooms. Still air box in the garage will get you more mushrooms than you can eat or give away. But like I said that's cool as shit and I wish I had the resources to even think about doing that.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: My Sterile Room [Re: Bph] 1
#26484731 - 02/14/20 05:50 AM (3 years, 11 months ago) |
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A for effort through
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Neowynd8

Registered: 06/12/16
Posts: 277
Last seen: 7 months, 1 day
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Re: My Sterile Room [Re: PTreeDish] 2
#26484775 - 02/14/20 06:23 AM (3 years, 11 months ago) |
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I think Stamets is widely regarded as a joke/sellout? in this community. Don't quote me lol
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: My Sterile Room [Re: Neowynd8] 2
#26484788 - 02/14/20 06:32 AM (3 years, 11 months ago) |
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No he's not, one guy on here likes to think he's like God because he posts 20 times a day on a message board and likes to shit on stamets when he hasn't accomplished 1/100th of what stamets has. When if they where in a room together this someone who claims to be so intelligent would get shit all over, just a shame I see so many new users listening to him - I can't blame them, he posts so much he just floods the boards and noobs think people with high post counts are cool.
Anyone with half a brain understands what was written 20 years ago should be taken with a grain of salt.
The personality ego cult is strong with that one, beware. He's working some shit job, not as a mycologist and isn't smarter than stamets.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Neowynd8

Registered: 06/12/16
Posts: 277
Last seen: 7 months, 1 day
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Quote:
filthyknees said: No he's not, one guy on here likes to think he's like God because he posts 20 times a day on a message board and likes to shit on stamets when he hasn't accomplished 1/100th of what stamets has. When if they where in a room together this someone who claims to be so intelligent would get shit all over, just a shame I see so many new users listening to him - I can't blame them, he posts so much he just floods the boards and noobs think people with high post counts are cool.
Anyone with half a brain understands what was written 20 years ago should be taken with a grain of salt.
The personality ego cult is strong with that one, beware. He's working some shit job, not as a mycologist and isn't smarter than stamets.
.
Don't quote me 😁 But seriously this guy says mentions stamets recommending 4 entrances to a inoculation room? I'm unaware of the accuracy, context, or intended audience of this but it sounds a bit ridiculous tbh...
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
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Re: My Sterile Room [Re: Neowynd8]
#26484853 - 02/14/20 07:20 AM (3 years, 11 months ago) |
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I do have a handsome post count don't I?
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: My Sterile Room [Re: Neowynd8]
#26484876 - 02/14/20 07:35 AM (3 years, 11 months ago) |
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Quote:
Neowynd8 said: Don't quote me 😁 But seriously this guy says mentions stamets recommending 4 entrances to a inoculation room? I'm unaware of the accuracy, context, or intended audience of this but it sounds a bit ridiculous tbh...
Haha don't worry I didn't! he wasn't talking to the guy growing some tubs in his apartment - that's for sure!
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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LeteciMedvedic
Srbin



Registered: 02/11/20
Posts: 17
Loc: Europe
Last seen: 3 years, 10 months
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Some patients using weed when it was illegal could hate Snoop Dogg, cypress hill etc. For using weed as the way to getting money, in the end, they all contributed to the understanding of weed... So isn't it same with stamets... He is earning money yeah, so is everybod on this planet...
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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A for effort surely. Lets hope your technique gets as much attention though.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 7 hours
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Quote:
PTreeDish said: I thought I'd share the sterile room I built in my attic. It's made from 3/4" PVC, 6 mil plastic and duct tape.
As far as locations go, it's probably one of the worst. My attic is full of white rot mold from old roof leaks and there are skylights everywhere that act serve as as ventilation. But this was the only spot the spouse would concede.
There is a small initial still air room that houses the entrance to the main room. The door is a Zip Wall.

Were you having problems with you flow hood? If its working right unless you are working a commercial levels this is just one cool as fuk set up thats really over kill. I do love it though.
Quote:
filthyknees said: No he's not, one guy on here likes to think he's like God because he posts 20 times a day on a message board and likes to shit on stamets when he hasn't accomplished 1/100th of what stamets has. When if they where in a room together this someone who claims to be so intelligent would get shit all over, just a shame I see so many new users listening to him - I can't blame them, he posts so much he just floods the boards and noobs think people with high post counts are cool.
Anyone with half a brain understands what was written 20 years ago should be taken with a grain of salt.
The personality ego cult is strong with that one, beware. He's working some shit job, not as a mycologist and isn't smarter than stamets.
I went away for a bit and came back to a bit of a split and Azur got booted for good. Are people falling out?
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PTreeDish


Registered: 04/22/18
Posts: 353
Last seen: 3 months, 15 days
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Re: My Sterile Room [Re: Edmunter]
#26485166 - 02/14/20 10:37 AM (3 years, 11 months ago) |
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Nah, no problems with the flow hood but I imagine it will extend its life given the tent is preventing the amount of dirty ambient air that must be filtered.
This is all for fun. Overkill? Maybe - probably. I'm growing large volumes and having this space blends practicality with the fun I'm having from the process. The tent cost < $50 to build.
The tent also creates a different vibe to work in. Good vibes are a key ingredient in my product. 
I'm new here so didn't realize the baggage this forum has with Stamets. For better or worse, his book (Guide to Growing Medicinal Mushrooms) was the first one I found over ten years ago and I use it, as well as many other sources, as a reference from time-to-time.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: My Sterile Room [Re: PTreeDish] 2
#26485254 - 02/14/20 11:19 AM (3 years, 11 months ago) |
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Blindingleaf one of the best TCs uses a prechamber before his popup tent where he houses his flow hood.
So extremely similar concept to yours. If you get confused on who has the good technique remember blindingleaf has run a gourmet farm sterile work for years. Most people here just have less than a dozen tubs going so I'd say it's clear who I'd listen to - the dude making money supporting himself for years that way. May not have heard because he's a modest person.
Most people here don't have a room to dedicate to a lab so don't think a prechamber like that hurts, most of the guys without lab rooms just wish they could be like bl, and he uses a prechamber so, maybe there are concepts that someone can grasp from that Paul stamets said with a critical mind /grain of salt, not taking it as gospel carved in stone.
Pmd you ed. Good luck op. May consider another kind of plastic door, I know those zippers can be tight especially if you have volume of things moving in and out.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 7 hours
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Re: My Sterile Room [Re: PTreeDish]
#26485827 - 02/14/20 05:40 PM (3 years, 11 months ago) |
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Quote:
PTreeDish said: Nah, no problems with the flow hood but I imagine it will extend its life given the tent is preventing the amount of dirty ambient air that must be filtered.
This is all for fun. Overkill? Maybe - probably. I'm growing large volumes and having this space blends practicality with the fun I'm having from the process. The tent cost < $50 to build.
The tent also creates a different vibe to work in. Good vibes are a key ingredient in my product. 
I'm new here so didn't realize the baggage this forum has with Stamets. For better or worse, his book (Guide to Growing Medicinal Mushrooms) was the first one I found over ten years ago and I use it, as well as many other sources, as a reference from time-to-time.
Im thinking of going commercial and would definitely do this. I have a plastic as entrance to mine but no room for this kind of thing yet.
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PTreeDish


Registered: 04/22/18
Posts: 353
Last seen: 3 months, 15 days
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Re: My Sterile Room [Re: Edmunter]
#26486380 - 02/15/20 01:04 AM (3 years, 11 months ago) |
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That's a cool setup and packs a lot of functionality in a small space.
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PTreeDish


Registered: 04/22/18
Posts: 353
Last seen: 3 months, 15 days
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You aren't wrong on the door (or your other remarks for that matter). I only used one of two of the zippers in the ZipWall set, thinking I might want a door on the prechamber, but I decided not so now I have an extra.
I've been lucky enough that the opening can get as wide enough to bring in a knee chair, some shelving, and supplies.
The other potential downside of the single zipper door is I've noticed it can create the same effect that you get when you put gloves on a SAB. The billowing effect of the plastic may be sucking in more dirty prechamber air than the positive ambient pressure of what the hood is putting out.
We'll see how it goes. I'm sure this thing will evolve in the coming months.
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Hobbyist
Citizen


Registered: 08/15/10
Posts: 805
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Re: My Sterile Room [Re: PTreeDish]
#26486522 - 02/15/20 05:17 AM (3 years, 11 months ago) |
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Quote:
PTreeDish said: When I unzip the main entrance, positive pressure from the hood running inside minimizes the introduction of additional contams upon entry.
Hey Brother, I like what you've done.
Just figured I'd mention that you won't have any positive air pressure unless your flowhood is pulling air from outside the clean room. If your FH intake is inside it'll just be blowing air around which will make a current, but not create positive pressure.
-------------------- Everything i say is completely hypothetical...
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: My Sterile Room [Re: Hobbyist]
#26486537 - 02/15/20 05:41 AM (3 years, 11 months ago) |
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That thing looks cool. Just don't fruit any oysters in there or the walls will go white with spores. This is one of those things I would end up building while I am waiting for jars to colonize or tubs to start to get ready to fruit. It is a great way to pass time and put in energy and focus, but overall it isn't as needed if you have a flowhood. I use to do all my agar work in one I built in my shed.
I didn't know azur got banned.. TO bad, I kinda liked his attitude.
Staments has produced more information and understanding of Mycology than any of us ever will. I have nothing bad to say about him.
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PTreeDish


Registered: 04/22/18
Posts: 353
Last seen: 3 months, 15 days
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Re: My Sterile Room [Re: Hobbyist]
#26487788 - 02/16/20 12:59 AM (3 years, 11 months ago) |
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Thanks man. Re: positive pressure. wiki defines it as:
Quote:
Positive pressure is a pressure within a system that is greater than the environment that surrounds that system.
When the zipper is open, the force of the fan pushes air into the prechamber, lowering the pressure of the sterilized room and increasing the pressure of the prechamber. This positive pressure would remain until the force of the fan was overcome by the outside air pressure.
Given the volume of the clean room and the fact that the zipper is both narrow and never open for more than a few seconds, wouldn't that pretty much ensure that most of the time, there is positive pressure into the unsterilized prechamber space?
The clean room would be in a slight vacuum when I zip the door up but would recede when the fan went off and outside could slowly leak in and/or I leave the door open.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: My Sterile Room [Re: PTreeDish]
#26487922 - 02/16/20 06:45 AM (3 years, 11 months ago) |
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I agree that if you don't have an area for the air to go into your flowhood from outside you don't have positive pressure.what is happening is when running the floowhood your slowly drawing air into your tent blowing it up probably via the zipper door. Otherwise you just have forced air moving around the inside of your tent. Plus you want laminar flow, so if your floowhood is blowing hard enough to create positive pressure with stagnat air inside, enough to force air out when you open the Zipper you might have to much flow to create laminar flow.
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Hobbyist
Citizen


Registered: 08/15/10
Posts: 805
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Re: My Sterile Room [Re: PTreeDish]
#26489337 - 02/17/20 02:30 AM (3 years, 11 months ago) |
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Quote:
PTreeDish said: Thanks man. Re: positive pressure. wiki defines it as:
Quote:
Positive pressure is a pressure within a system that is greater than the environment that surrounds that system.
When the zipper is open, the force of the fan pushes air into the prechamber, lowering the pressure of the sterilized room and increasing the pressure of the prechamber. This positive pressure would remain until the force of the fan was overcome by the outside air pressure.
Given the volume of the clean room and the fact that the zipper is both narrow and never open for more than a few seconds, wouldn't that pretty much ensure that most of the time, there is positive pressure into the unsterilized prechamber space?
The clean room would be in a slight vacuum when I zip the door up but would recede when the fan went off and outside could slowly leak in and/or I leave the door open.
You are correct in the quote you posted, however: If you think in terms of sealed systems it might be easier to explain.
Imagine your clean room is an actual room and airtight. Now, put your flow hood in the room(or any fan). Your clean room in it's current state with all the equipment and air inside it can be considered a sealed system. If you turn on the fan with the room sealed and secure, you will move around the air in the room and create turbulence and moving air inside that room, but you will only be moving the air that's already inside that room. As you have not introduced anything additional to the sealed system, there's no reason to think that the pressure inside would be greater.
Since you aren't introducing any additional air, you won't be creating and pressure, just air currents. In order to increase the pressure in the room, you must add to the contents of the room, change the temperature of the room, or change the room's volume in some way.
Since the only thing you're doing is creating airflow with your flow hood you aren't creating pressure, you're simply moving air.
However
If you'd like to create positive pressure it would be easy: adjust your setup so your flow hood pulls air from outside your clear room. Try it and consider the difference. I wager your plastic clean room becomes bubble-esque with the positive pressure.
If you have your flow hood intake draw air from outside the clean room, you will be adding all the air your flow hood filters to the clean room, thus increasing the volume of air for the given volume of the room creating positive pressure.
-------------------- Everything i say is completely hypothetical...
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 6 hours, 19 minutes
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Re: My Sterile Room [Re: PTreeDish]
#26489579 - 02/17/20 07:51 AM (3 years, 11 months ago) |
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Quote:
PTreeDish said: Stamets book recommended a proper clean room having up to 4 entrances before entering the final room. So in my model, I created a still air entrance room that is about 4x4 that houses the zip wall. So it's basically one additional place to minimize introducing dirty attic air.
When entering the first entrance, there is 70% IPA and gloves to wipe myself down and everything around me down. When I unzip the main entrance, positive pressure from the hood running inside minimizes the introduction of additional contams upon entry.
I've also taken measures to clean and sterilize the pathway I take outside of the tent to get to it. Finally, I have some degree of climate control in this room and have the flexibility of tapping an outside air source and directing it only into this space when needed.
Is any of this necessary? I don't know but I could not, in good conscience, simply work in the open air of my attic.
Not really. Because the air coming out of the flow hood is moving in a nice straight line, assuming it's working properly, you don't actually need any kind of physical barrier around that area to keep contaminants out. The movement of the air itself prevents contaminated air from surrounding area from entering the area directly in front of the hood. So when you're using a FH all you really need to worry about it your sterile technique because even though the air coming out of the hood is practically sterile the surfaces of the objects you're placing into that air flow are not. The main cause of contamination is going to be handling things in a way that allows a nonsterile surface, like your hand, to come between the front of the hood and a sterile surface, like the inside part of a petri or jar lid.
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rm1024
Familiar Stranger
Registered: 11/05/18
Posts: 62
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Re: My Sterile Room [Re: Kizzle]
#26489650 - 02/17/20 08:58 AM (3 years, 11 months ago) |
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PTreeDish


Registered: 04/22/18
Posts: 353
Last seen: 3 months, 15 days
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Re: My Sterile Room [Re: rm1024]
#26490987 - 02/18/20 12:39 AM (3 years, 11 months ago) |
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Appreciate the clarification on positive pressure and the value (or lack thereof) in a confined space for sterility. There other benefits to the enclosed space, like keeping the attic dust and mold out, but good to know it's really the sterile laminar flow and sterile technique that matter most.
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