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OfflineZillard
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Registered: 01/28/20
Posts: 16
Loc: South Africa
Last seen: 3 years, 9 months
What the fluff?
    #26481730 - 02/12/20 10:32 AM (4 years, 4 days ago)

Hi all

I have petri dishes with some liquid culture going. Have gotten decent growth 4 days after inoculation. However, the mycelium is very fluffy. And instead of spreading out sideways, it is growing upwards, kind of like a dome.


Is this normal growth? I will greatly appreciate input, as I'm still learning.

Much appreciated.


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OfflineKmacmo
The aborted pin
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Registered: 08/14/19
Posts: 1,679
Loc: Central hemisphere
Last seen: 7 hours, 35 minutes
Re: What the fluff? [Re: Zillard]
    #26481802 - 02/12/20 11:17 AM (4 years, 4 days ago)

Hey, you used LC to inoculate the agar dish or a spore Syringe?

I'd take a few transfers from 1-2pm on the left colony and some from 4pm on the right colony.

Looks normal to me


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
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Re: What the fluff? [Re: Kmacmo]
    #26481818 - 02/12/20 11:28 AM (4 years, 4 days ago)

My lc test plate did the same thing, looks good to me.


--------------------
LAGM2020


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OfflineWeavieWonder
Time Travel Sucks
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Registered: 08/18/16
Posts: 1,455
Loc: USA Flag
Last seen: 1 month, 21 days
Re: What the fluff? [Re: A.k.a]
    #26481854 - 02/12/20 11:48 AM (4 years, 4 days ago)

Quote:

A.k.a said:
My lc test plate did the same thing, looks good to me.




:whathesaid: you're fine.


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OfflineZillard
Stranger

Registered: 01/28/20
Posts: 16
Loc: South Africa
Last seen: 3 years, 9 months
Re: What the fluff? [Re: A.k.a]
    #26481857 - 02/12/20 11:50 AM (4 years, 4 days ago)

Oh, I used some LC syringes I got from a supplier. 1 or 2 drops per plate, but ended up swirling them around a bit to spread it out more.

Thank you for your feedback.

I will give 2 more days to see more growth, then cut a few wedges out of the edges and transfer over to fresh plates. Thanks for your location suggestion on where to grab them.

However, I believe there is some cobweb mold growing (barely visible top right corner)close to those areas. Hopefully it wont be too close to the mycelium when I want to transfer out.

Of the 3 plates, that seems to be the only contamination I can see. Hoping the leftover LC in the syringe wont give me issues in future.

Thanks guys


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