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Offlinemaniac1886
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Registered: 10/05/11
Posts: 170
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Last seen: 2 days, 2 hours
Cleaning a contaminated monotub for re-use
    #26481569 - 02/12/20 08:14 AM (3 years, 11 months ago)

TL/DR  ->  Skip to last paragraph

Ive been having a real rough go the passed couple months with contamination.  I *THINK* I have narrowed down the issue, however, as I tighten up my tek, but it has also brought up some questions.  quick rundown.

I have been 100% successful on my last run, which spanned about a year.  No contams, no issues.  Then, I started running into hella issues.  Like, 100% failure rate.  I have decided that a large number of my issues stemmed from the temperature change, and the room I have been operating in.  Since it started getting cold, and the furnace has been running, the room I was operating in does not maintain temp well.  So I was getting HEAVY condensation on the lid of the tub.  I found that I was gettin Trich contams where the water was dropping onto the substrate, and the top of the sub was way too wet.  Visible water drops were also forming on the surface.  My jars were also in this room, and I fear they have also fallen victim of condensation introducing mold. So long story short I am tightening up my tek. 

I started using a meat thermometer on a remote probe to more effectively pastuerize my sub.  Before I used the bucket tek basically.  I would add my water to the pressure cooker, bring it to a boil, drop the coir in, put a lid on it, and wrap a towel around it.  This has ALWAYS worked.  Now, in an effort to eliminate bad sub I have begun to monitor and maintain temp during the pasteurization period.

I created a new LC from a MS of a different variety (went from B+ to Huahuatla).  I tested the LC in 4 BRF jars.  All were fine.  I've also since started an agar plate of both of the LC's I made.  First plate, minor bacteria, as expected.  Im now 4 days into a second transfer (3rd plate) and both cultures look clean and healthy thus far.

When I spawn my tub, I will typically add the liner, and dump in the sub while its still around 100 degrees.  I take the tub into a bedroom to cool a few degrees.  When Im ready to spawn I will cover the register, turn off the fan and gas the room with Lysol, then walk away for 15 minutes.  I will Wash my hands/arms up to my elbows then return to the room with the spawn, and dump each jar in before mixing it all around.

I feel like my tek is solid.  I've had a real good go at it up until it started getting cold.  To mitigate that going forward, I have a small room that I built years ago, fits a few tubs, a heater, and a light.  the small room is insulated and has a digital thermostat that turns the heater on and off to regulate temp.  Works phenomenally.  Always has.  I figured I would move my operation back to that room to keep a more consistent temperature.  there is a 6500k CFL bulb in the room, that stays on.  It hangs about 1 foot above the tubs.

So here, is my question.  With the frustrating go that I have been having, I have been having a bad habit of just throwing the contammed tub out on the back porch and forgetting about it.  By the time i go to use it again, Trich has COMPLETELY taken over the tub.  I dump it out in the back yard, spray it down and bring it in the house.  I will then cover the entire bin and lid with a HEAVY coat of lysol, and set it in an empty bath tub for 10/15 minutes.  I come back, rinse well with hot water then wash it with soap, hot water and sponge.  I typically will wipe out the excess water with fresh paper towel then let it air dry.  The question is, is this enough?  Are there nasties still clinging to the lid and walls?  Does anything KILL trich spores?  I know this a bad habit I've formed.  If shit would stop contamming, then I wouldnt even do it.  Should I get new bins and start fresh?  I would really prefer to keep the ones I have but I am beginning to fear they have been contammed beyond repair.


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Offlinefeldman114
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Registered: 09/06/19
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Re: Cleaning a contaminated monotub for re-use [Re: maniac1886] * 1
    #26481572 - 02/12/20 08:16 AM (3 years, 11 months ago)

You don’t need to kill trich spores cause they’re all over your house.

If you wanna be safe, bleach the tub. I just wash with soapy water...than again, I always toss tubs at first sign of green.


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Offlinemaniac1886
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Registered: 10/05/11
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Re: Cleaning a contaminated monotub for re-use [Re: feldman114]
    #26482055 - 02/12/20 02:08 PM (3 years, 11 months ago)

I appreciate your response, and I understand and accept that concept.  However, when spawning a tub, i do what I can to mitigate that thru multiple angles.  Such as lysoling the room and allowing the lysol to settle, as well as blocking vents and shutting off fans to create a still air environment.  It's my understanding that these things are done to cut down on floating contamination. 

Obviously some of those contaminants are going to be inside the tub as it is in open air while drying, and while installing a liner and dumping substrate in.

What I am afraid of is that large colonies of Trich are capable of attaching themselves to the pores in the plastic and or stick to it from a wicked infestation and have a higher potential for taking hold in the sub once the lid is on and the warm humid air fills the tub versus the Trich spores that passively fall into it from air "pollution"

Does that make sense?  Or am I completely off base?


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Offlinefeldman114
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Re: Cleaning a contaminated monotub for re-use [Re: maniac1886]
    #26482084 - 02/12/20 02:26 PM (3 years, 11 months ago)

Quote:

maniac1886 said:
I appreciate your response, and I understand and accept that concept.  However, when spawning a tub, i do what I can to mitigate that thru multiple angles.  Such as lysoling the room and allowing the lysol to settle, as well as blocking vents and shutting off fans to create a still air environment.  It's my understanding that these things are done to cut down on floating contamination. 

Obviously some of those contaminants are going to be inside the tub as it is in open air while drying, and while installing a liner and dumping substrate in.

What I am afraid of is that large colonies of Trich are capable of attaching themselves to the pores in the plastic and or stick to it from a wicked infestation and have a higher potential for taking hold in the sub once the lid is on and the warm humid air fills the tub versus the Trich spores that passively fall into it from air "pollution"

Does that make sense?  Or am I completely off base?




No offense, but you’re doing a lot of extra work for no reason.
Lysol doesn’t kill spores. More importantly, spores don’t germinate on coir or on colonized grain. So, if you have clean spawn and are using coir/verm for sub, it doesn’t matter how many spores get in there.

What you described are great precautions to take before doing SAB work though. That’s when you do need to keep shit sterile.


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Offlinemaniac1886
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Registered: 10/05/11
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Re: Cleaning a contaminated monotub for re-use [Re: feldman114]
    #26482228 - 02/12/20 03:47 PM (3 years, 11 months ago)

Yeah, those precautions actually adopted from how I do SAB work. Funny you mention that.  I just have been going down a rough path lately and I'm trying to tighten up my procedure in every aspect in order to break out of the rut.  I've been doing this stuff on and off (mostly on) for almost 10 years and for whatever reason, the passed few months have been like nothing I've ever experienced.  Im actually about to clean up a bin and birth some jars that have been started from scratch.  Everything I had going has been gotten rid of.  No more old grains that were in that room, no B+ LC, New lc, clean grains, more accurately monitored sub pasteurization.  The only thing I haven't basically started from scratch is the jars and bins.  I'm not to worried about jars as they get PCed but I don't want to put in more time and effort for another Trich invasion.  I was hoping to hear some insight about a lingering contam inside a bin but it sounds like as long as I do my due dilligence the bin should not be the issue at hand.

I'm racking my brain on every detail no matter how minute because I wanna eat some fucking shrooms already!

I but the bullet and bought a quarter from someone on new years and me and my chick were both very disappointed with the quality.  Waste of fucking money on something I, for the most part, refuse to even purchase. Lol


Thanks for your insight.  If I remember Ill update this thread with the results.


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Offlinefragrantdiscipline
LostWanderer


Registered: 01/02/20
Posts: 32
Last seen: 3 years, 2 months
Re: Cleaning a contaminated monotub for re-use [Re: maniac1886]
    #26482424 - 02/12/20 06:11 PM (3 years, 11 months ago)

Quote:

I would add my water to the pressure cooker, bring it to a boil, drop the coir in,




Bod's simple technique for coir pasteurization works. There's no need to go overboard. Your contamination is likely coming from somewhere else.

Quote:

gas the room with Lysol




This is an ineffective method to control contamination in your work space. Contamination is already everywhere. You can't clean your house well enough. That's what the SAB is for.

Contamination is always present. You just have to accept it. Inoculation aside because it requires sterility,  what you're trying to do is reduce the pathogens to the point that the mycelium can overcome it.

One thing you haven't outlined in your tek is your clothing gear. Are you masked? Is your hair covered? Are you trimming and cleaning your finger nails? Are you wearing gloves? A microbiology 101 course will demonstrate the ease with which cultures can be contaminated simply from your breath. If you're not covering your nose, mouth & hair and wearing gloves, that's likely where your contamination is coming from.


--------------------
Desperately in need of some stranger's hand in a desperate land.


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Offlinefeldman114
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Registered: 09/06/19
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Re: Cleaning a contaminated monotub for re-use [Re: fragrantdiscipline]
    #26482801 - 02/12/20 09:14 PM (3 years, 11 months ago)

That’s horrible advice at the end there...this is ghetto mycology, not microbiology 101:rasta:
And any ghetto mycologist will tell you that you can sneeze, cough, and fart on clean spawn and coir - it’ll be just fine.

But I have a feeling OP knew that lol.
OP, I’d top fruit a couple jars if I were you (quicker to the finish line)


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Offlinefragrantdiscipline
LostWanderer


Registered: 01/02/20
Posts: 32
Last seen: 3 years, 2 months
Re: Cleaning a contaminated monotub for re-use [Re: feldman114]
    #26483105 - 02/13/20 04:28 AM (3 years, 11 months ago)

Quote:

That’s horrible advice at the end there




I'm just going to point out that the gentleman has problems with contamination from undetermined sources. A stricter protocol might not be the "horrible advice" you believe it to be until the source of contamination is identified. Since his post seems to indicate the possibility of a pattern, a process of elimination (or rather minimization since contaminates can't be completely eliminated) would be useful if for something like mold and fungus under the dude's finger nails with which he uses to pick that pesky booger from his nose and then straighten his hair thereby inoculating the areas I'm advising that he cover up.

But, what do I know?

I'm not saying the gentleman is un-hygienic. I'm just saying we can minimize the possibility that he's inadvertently introducing contaminates. Garbing up is a convenient, easy way to minimize a possibility so that he can look to other sources for the contamination.


--------------------
Desperately in need of some stranger's hand in a desperate land.


Edited by fragrantdiscipline (02/13/20 04:31 AM)


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