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OfflineIgloomis
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Very frustrated. Please advise
    #26481516 - 02/12/20 07:33 AM (4 years, 4 days ago)

I did two grows using Quaesitor Tek and a reliable mail order spore source. I was overjoyed with the results. The third time I had to go out of town during the substrate colonization. When I came back all had been lost to gray mold.

On my fourth grow now and it is going into a nosedive. I did two tubs, one with my own substrate mix and the other with one mail ordered from someone we all know and love. About a week and a half into colonization I wondered if my problem had been not enough ventilation (lid on holes sealed) so I started letting it breath for a few seconds each day. But it soon appeared that colonization had come to a standstill. Weeks later there is very little advancement of mycelium and I'm seeing small spots of green.

Any observations?


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Offlinefeldman114
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Re: Very frustrated. Please advise [Re: Igloomis] * 2
    #26481526 - 02/12/20 07:40 AM (4 years, 4 days ago)

Wtf is Quaziator tek?

You done fucked up:shrug:

Just start from scratch - put some spores on agar and make some good filtered lids. And while your agar grows you can read up on sterile tek and a fruiting tek that doesn’t suck.


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Invisiblegizmo1
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Re: Very frustrated. Please advise [Re: feldman114]
    #26481611 - 02/12/20 08:58 AM (4 years, 4 days ago)

Quote:

Igloomis said:
I did two grows using Quaesitor Tek and a reliable mail order spore source. I was overjoyed with the results. The third time I had to go out of town during the substrate colonization. When I came back all had been lost to gray mold.

On my fourth grow now and it is going into a nosedive. I did two tubs, one with my own substrate mix and the other with one mail ordered from someone we all know and love. About a week and a half into colonization I wondered if my problem had been not enough ventilation (lid on holes sealed) so I started letting it breath for a few seconds each day. But it soon appeared that colonization had come to a standstill. Weeks later there is very little advancement of mycelium and I'm seeing small spots of green.

Any observations?



If you would like to upload some pics we would be happy to help you and make observations.

Quote:

feldman114 said:
Wtf is Quaziator tek?




Idk but sounds kinda cool lol.


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OfflineRaccoon
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Re: Very frustrated. Please advise [Re: gizmo1]
    #26481637 - 02/12/20 09:18 AM (4 years, 4 days ago)

You're seeing spots of green, or you saw spots of green? If you are seeing green, throw out the sub. If you saw green, then I you already tossed the sub. Take a picture and post here if you're not sure, but do it now. As far as what went wrong, nobody knows what quausitor Tek is, haha.


--------------------
First Grow


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Invisiblegizmo1
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Re: Very frustrated. Please advise [Re: Raccoon]
    #26481739 - 02/12/20 10:39 AM (4 years, 4 days ago)

The green could just be some bruising going on or something never know what the untrained eye is seeing without pictures.

I've searched quaziator here and no results well besides this thread. So I typed it into duckduckgo search engine and no results were found. Im not really sure if maybe that was a typo or something.


Edited by gizmo1 (02/12/20 10:46 AM)


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Invisiblealaskappalachian
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Re: Very frustrated. Please advise [Re: gizmo1]
    #26481753 - 02/12/20 10:49 AM (4 years, 4 days ago)

Apparentky a person called Principium Quaesitor authored some book called "Magic Mushroom Grower's Guide Simple Steps to Bulk Cultivation."  Never heard of it. :shrug:


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Invisiblegizmo1
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Re: Very frustrated. Please advise [Re: alaskappalachian]
    #26481769 - 02/12/20 10:58 AM (4 years, 4 days ago)

Well you got further than I did with searching lol. We're that much closer to decoding the tek used.
I didn't see ot was spelled differently in OP than in the first reply no wonder I didnt find shit. :facepalm:


Edited by gizmo1 (02/12/20 10:59 AM)


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OfflineSockadin
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Re: Very frustrated. Please advise [Re: alaskappalachian]
    #26481777 - 02/12/20 11:03 AM (4 years, 4 days ago)

No E-book available. Only paperback and written in 2014. I was just curious how many ideas where borrowed from this website. I might order it for fun.


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OfflineIgloomis
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Re: Very frustrated. Please advise [Re: Sockadin]
    #26485033 - 02/14/20 09:26 AM (4 years, 2 days ago)

Yes, that's the book. I was hoping someone was familiar with it.

My problem is not sterile conditions. I have a closed sterile lab and SAB. I have grown dozens of jars of spawn and never had any contamination, and I have had very successful grows. I think my error this time (and the previous, now that I think about it) was sealing my monotubs too tightly during bulk substrate colonization. The colonization seemed to slow to a crawl and possibly gave opportunity for contamination (if that's what it is).

Does that seem likely?

Here are the spots I saw. I removed them and they have not returned. Even though the mycelium was not thickly colonized due to slowed growth I decided to start fruiting to see what would happen. By this point the substrate looked very dry, so I am spritzing the tub walls regularly. Pins have developed (although sparsely) and are growing normally. I live in the desert and the air is horrifically dry this time of year. In the past, if growing in winter, my second flush was always much more productive after thorough rehydrating.

Question: If you suspect slight contamination but it doesn't progress noticeably is it safe to assume the mushrooms are OK, or is it a total loss anyway? At this point I'm considering it a learning opportunity regardless of if/what I harvest.



Edited by Igloomis (02/14/20 09:35 AM)


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OfflineRaccoon
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Re: Very frustrated. Please advise [Re: Igloomis]
    #26485050 - 02/14/20 09:35 AM (4 years, 2 days ago)

I think most would tell you to toss that sub. But if you removed the spots and your grow seems to be moving along, them let it grow. That mold is most likely not-pathogenic. However, I would quarantine that grow from your grow area... Maybe someone with more experience can chime in, but from all I read, once you see green, you don't have much time before your whole sub is green no matter what you do. Cutting it out, only buys you time to push out some fruits. Keep us posted on your progress, good luck.


--------------------
First Grow


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Offlinefeldman114
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Re: Very frustrated. Please advise [Re: Igloomis]
    #26485052 - 02/14/20 09:36 AM (4 years, 2 days ago)

Ooof, that tub is fucked, bud.

The mean green takes no prisoners:sad:


But it prolly came from your spawn. The oxygen inside a tub should be enough for colonization.


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OfflineIgloomis
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Re: Very frustrated. Please advise [Re: feldman114]
    #26485375 - 02/14/20 12:23 PM (4 years, 2 days ago)

Thanks for your feedback, everybody.

It makes sense that it came from the spawn but I carefully inspected it as I added it to the substrate and saw nothing.

Any credence to my theory of too much CO2 buildup in the early phase of colonization? My tubs have locking lids, which in my successful grows I left loose. The only other time I had contamination was when I went out of town and left the lids locked for a couple of weeks, all ventilation holes taped shut.


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Offlinefeldman114
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Re: Very frustrated. Please advise [Re: Igloomis]
    #26485379 - 02/14/20 12:27 PM (4 years, 2 days ago)

Did you carefully inspect the agar plate you used to knock up your spawn?
How many transfers did you make?

Clean =\= visibly clean


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OfflineIgloomis
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Re: Very frustrated. Please advise [Re: gizmo1]
    #26485399 - 02/14/20 12:41 PM (4 years, 2 days ago)

Gizmo1 said, "Well you got further than I did with searching lol. We're that much closer to decoding the tek used.
I didn't see ot was spelled differently in OP than in the first reply no wonder I didnt find shit. "

Sorry about that. This tek uses birdseed for spawn soaked with gypsum and coffee then sterilized in mason jars, and a bulk substrate of manure, coir, vermiculite, gypsum and coffee grounds in monotubs with 6 holes (taped over during substrate colonization, then top holes loosely packed polyfill and bottom tightly packed).

It seems to be generally similar to what I'm seeing here and I have had great results in the past.


Edited by Igloomis (02/14/20 12:51 PM)


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Offlinefeldman114
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Re: Very frustrated. Please advise [Re: Igloomis]
    #26485423 - 02/14/20 12:57 PM (4 years, 2 days ago)

Ooh ok, I see the problem now.
See, using outdated info will get you in trouble because mush cult is a fairly new “science”. It has evolved in major ways in the past 5 years alone.

If you check the date on the tek that lists coffee as an ingredient, I guarantee you’ll find that it’s 5-10 years old, if not more.
Likewise, it was recently shown that light is beneficial during colonization, and that introducing “fruiting conditions” right after spawning reduces the wait for pins.


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Offlinefeldman114
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Re: Very frustrated. Please advise [Re: feldman114]
    #26485427 - 02/14/20 12:59 PM (4 years, 2 days ago)

Forgot the most important thing - learn agar. Until you see your culture on agar, you can’t possibly be sure it’s clean. Many contams are invisible on grain.


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OfflineIgloomis
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Re: Very frustrated. Please advise [Re: feldman114]
    #26485462 - 02/14/20 01:18 PM (4 years, 2 days ago)

Quote:

feldman114 said:
Did you carefully inspect the agar plate you used to knock up your spawn?
How many transfers did you make?

Clean =\= visibly clean



No agar. I injected from mail-ordered syringes (flame sterilized before each injection) through self-sealing inoculation ports on sterilized jars. The jars were moved directly from the sealed pressure cooker to SAB which had been sterilized with disinfectant and lined with alcohol-soaked paper towels. I sterilized the entire room (dedicated workspace with plastic lined walls and ceilings) beforehand and misted the air with disinfectant. I wore a clean lab coat, cloves, mask, and even used hand sanitizer on top of the gloves. Sprayed the lab coat sleeves with disinfectant before reaching into SAB. Other than a ventilation hood I can't imagine any way to make this procedure more sterile.

That's why I'm narrowing my focus to something going wrong in the substrate colonization phase. I even did one tub with my own substrate mixture and purchased pre-mixed/moistened/pasteurized substrate from one of the sponsor companies for a comparison.


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OfflineIgloomis
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Re: Very frustrated. Please advise [Re: feldman114]
    #26485472 - 02/14/20 01:25 PM (4 years, 2 days ago)

That's good to know. I have agar and petri dishes but haven't made that leap yet. My syringes come from a prominent sponsor of this forum. What do you think is the likelihood of impurity in such a source?

BTW, thanks so much for your help, guys. I feel like I'm alone in the wilderness doing this stuff. I am growing at my son's request because it has proven helpful for his depression.


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InvisibleSmartattack
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Re: Very frustrated. Please advise [Re: Igloomis]
    #26485497 - 02/14/20 01:42 PM (4 years, 2 days ago)

Your in blind luck if you use a syringe and don't get contam. I started with syringes as well. Had contamination. Stopped using syringes, zero contamination. They really are regularly that bad. Reputable source or not. It's not even their fault, as used correctly (agar) syringe contam isn't an issue anyways. Straight to grain with it is the problem.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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Offlinefeldman114
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Re: Very frustrated. Please advise [Re: Smartattack]
    #26485505 - 02/14/20 01:50 PM (4 years, 2 days ago)

I think the Russian roulette analogy works best here. Cause, if I’m being honest, 4/5 times ms>grain will work...but that one time is gonna be a biiigity bitch.

I was kinda over the whole agar “fad” for a while, until I got a bunk syringe. 7 masters went to shit and I had empty monos waiting for spawn to colonize for weeks. Sooo not worth all the previous success.

Plus, you can’t clone well without agar, and cloning is a game-changer for anyone using ms>grain.


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