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OfflineBassnectar808
Registered: 06/30/18
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Last seen: 3 years, 1 month
Questionably Slow Tomentose Mycelium Growth On Agar
    #26480998 - 02/11/20 08:17 PM (3 years, 11 months ago)

I realize the pictures are blurry and a bit difficult to see fully. However, I'm a bit concerned with the rate and style of growth present. This plate was inoculated 7 days ago as the first transfer from an agar dish that was inoculated with an A+ albino MS syringe from a sponsored vendor. This initial inoculation also took a long while to grow. There were eight other plates prepared, all with similar slow tomentose mycelium growth. None appear to exhibit any strong rhizomorphic patterns. Is this time frame/growth style something I should be concerned with or should I continue to let these grow/isolate and use for a master grain jar?

The average temperatures during weekdays do get in the mid 60's when at work but are back up to mid 70's when home.

Also will add this is only my second attempt at agar and first time trying to actually isolate clean genetics for use. The other time was simply a trial experiment, which actually resulted in very clean rhizo that ended up fruiting in the plates after some time left in a cabinet.







Edited by Bassnectar808 (02/11/20 09:27 PM)


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OfflineSockadin
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Registered: 01/03/10
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Re: Questionably Slow Tomentose Mycelium Growth On Agar [Re: Bassnectar808]
    #26481056 - 02/11/20 08:50 PM (3 years, 11 months ago)

Nah your good, some varieties just grow out slower. If the nutrients are denser and it doesn't need to run for food it will sit for a while.


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OnlineNichrome
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Registered: 12/17/18
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Re: Questionably Slow Tomentose Mycelium Growth On Agar [Re: Sockadin]
    #26481148 - 02/11/20 09:46 PM (3 years, 11 months ago)

Looks peachy. Just go with it.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineBassnectar808
Registered: 06/30/18
Posts: 3
Last seen: 3 years, 1 month
Re: Questionably Slow Tomentose Mycelium Growth On Agar [Re: Nichrome]
    #26484022 - 02/13/20 04:21 PM (3 years, 11 months ago)

Thanks for the reassurance!

Few other questions:

1) How many transfers away from ms are recommended before going to grains. I believe I've seen try for a minimum of 3 successfully clean transfers.

2) Due to the slow growth possibly arising from dense nutrient agar, would it be advised to lessen the nutrient density to try and coax the mycelium to spread?

This was made with PDA using pastywhyte's recipe. I made 9 plates

Using:240ml water, 6g agar powder, 6g of potato flakes ground to powder (weight of 4tsps potato flakes not ground, and a drop of karo.


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OfflineMooshi97
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Registered: 02/16/19
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Re: Questionably Slow Tomentose Mycelium Growth On Agar [Re: Bassnectar808]
    #26484179 - 02/13/20 06:15 PM (3 years, 11 months ago)

That is a lot of potato flakes for 9 dishes imo. I use 3.5 grams per 9 dishes and my agar dishes grow at a reasonable rate, but genetics are a factor as well.


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OfflineSockadin
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Re: Questionably Slow Tomentose Mycelium Growth On Agar [Re: Mooshi97]
    #26484184 - 02/13/20 06:21 PM (3 years, 11 months ago)

Might be the Karo, I would do 6 nutrient 12 Agar 250 Water.. Or something like that, but what do I know I use LME most of the time or Grain water if i am smart enough to save some.


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