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InvisibleDeckardCain
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Registered: 09/22/12
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How many transfers from a clone before a2g
    #26480536 - 02/11/20 03:48 PM (3 years, 11 months ago)


These are some 2nd transfers I have, I had some issues with my agar recipe but these looks to be growing fluffy white and better, will 2 transfers be enough?
People say you want to make sure it’s clean before putting it to grain, but how can you tell other than seeing obvious contaminates?
I have a few more plates but they have contaminates. since changing rooms I seem to have a harder time getting clean transfers.

And yes obviously I’ll let the plates grow out more before a2g I’m just curious what all I should be keeping an eye out for. Thank you


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InvisibleSmartattack
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Registered: 12/21/18
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Re: How many transfers from a clone before a2g [Re: DeckardCain]
    #26480570 - 02/11/20 03:57 PM (3 years, 11 months ago)

Its already a clone. No need to transfer a bunch, you just want it clean. Last time I cloned, my initial plate with tissue sample was fine so i just dropped the whole biz to grain.

That looks great really.


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Invisibleeatyualive
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Re: How many transfers from a clone before a2g [Re: Smartattack]
    #26480904 - 02/11/20 07:21 PM (3 years, 11 months ago)

If you have even growth inoculate. You would likely transfer at this point to keep the culture going.


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