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OfflineA.k.aM
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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26479062 - 02/10/20 06:22 PM (4 years, 7 days ago)

Wait are you pour or no pours?

For looking at it and pictures you’ll have to just look through the glass.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: A.k.a]
    #26479084 - 02/10/20 06:37 PM (4 years, 7 days ago)

No pour. Am I going to be able to see the growth with detail through the glass?Damn man I think I’m going to have to do it all over because I poured a relatively thick amount, so looking through the glass I don’t have a clear side view angling down. I’m at eye level with it. I guess to monitor through the glass I have to have an angle looking down at the bottom of the jar. Fuck. I’m going to have to do this all over again tomorrow. Fuck. My. Life. This sucks.


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Edited by psycho_nauticus (02/10/20 06:52 PM)


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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26479105 - 02/10/20 06:51 PM (4 years, 7 days ago)

Did the pictures on the top clear up? Like was it just LME getting to hot and caramelizing ??


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: Sockadin]
    #26479108 - 02/10/20 06:53 PM (4 years, 7 days ago)

I don’t know, this was my very first time doing it. I was asking for an explanation myself. It looks like I’m going to have to do everything over anyways because I don’t have a good aerial view from the side of the glass because I put too much in each one, and I made them all like that. That frothy bubble stuff I don’t know, it’s going to obstruct visibility.

I’m just getting hella frustrated. It’s taking a lot out of me, money wise also getting all this stuff, and now I have to go buy more jars again and do everything over. It’s going to take me messing up each time and having to do everything over time and time again in order for me to finally get it right one time. I’m feeling angry and exhausted.


Edited by psycho_nauticus (02/10/20 07:20 PM)


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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26479255 - 02/10/20 08:26 PM (4 years, 7 days ago)

Those are poured wayyyyy too thick you want maybe a 1/4 inch in the bottom. The bubbles on the top are fine they are usually gone by the time they come out of the pc.
It looks like the jars are about half way full? They will still work but it will make it very hard to see the growth.
You want them about this thick. If you were using petris even less but jars have that hump in the bottom that you have to pour over.



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Edited by gizmo1 (02/10/20 08:30 PM)


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Re: Agar for a noob like me? [Re: gizmo1]
    #26479261 - 02/10/20 08:29 PM (4 years, 6 days ago)

Gizmo, can someone use Glass jars and plastic lids and pour them and flip them upside down to harden in the plastic lid? IDK if that would even work, just thinking about a way to have some visibility in the no pour process.


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Re: Agar for a noob like me? [Re: Sockadin]
    #26479264 - 02/10/20 08:35 PM (4 years, 6 days ago)

Quote:

Sockadin said:
Gizmo, can someone use Glass jars and plastic lids and pour them and flip them upside down to harden in the plastic lid? IDK if that would even work, just thinking about a way to have some visibility in the no pour process.



I want to say no not with these containers. I saw where one guy did it in some mini rounds but those have a little recess in the middle of the lid where the agar wont go all the way to the edge.
No pours kinda are what they are if you want better visibility the best thing to do it switch to actual petris or try the no pour containers from aliens holy grail tek or  even deli cups.


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Re: Agar for a noob like me? [Re: gizmo1]
    #26479266 - 02/10/20 08:36 PM (4 years, 6 days ago)

Oh yeah and when you open the lid there would be agar everywhere.... Duhhhh  I just had a :facepalm: moment.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26479609 - 02/11/20 06:11 AM (4 years, 6 days ago)

Thanks Gizmo, I’m going to do them over again today. I realized that yesterday when talking with Aka that I wouldn’t be able to see the growth because of how much I poured. Hopefully this second time round everything will come out correct. I also read on some other thread last night 7 gram agar/10 grams LME brings the culture out better... What does this mean? Do you think I should go with that mixture this time around?


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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26479647 - 02/11/20 07:03 AM (4 years, 6 days ago)

Quote:

psycho_nauticus said:
Thanks Gizmo, I’m going to do them over again today. I realized that yesterday when talking with Aka that I wouldn’t be able to see the growth because of how much I poured. Hopefully this second time round everything will come out correct. I also read on some other thread last night 7 gram agar/10 grams LME brings the culture out better... What does this mean? Do you think I should go with that mixture this time around?



Im not sure what thats supposed to mean but you can always try it it won't hurt. 7 grams agar is a little soft which is fine and 10 grams malt extract is a little high which is also fine. For the longest time I used 10 grams of each to 500 mL water.
Also if 500ml agar is too big of a batch for your vessels you can always half everything and do a 250mL batch. That way you don'thave to overfill to get rid of it all lol.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26479677 - 02/11/20 07:33 AM (4 years, 6 days ago)

Yeah I was thinking about doing that because there was a lot left over also. So wouldn’t it be better for me to get a little bigger wide mouth jar than the 4 oz ones that way there is more surface area for it to grow and spread whenever I have to take the edges for transfers and stuff?


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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26479873 - 02/11/20 09:58 AM (4 years, 6 days ago)

It works there’s a guy who just did it recently. I want to say wizard was in the name. But he said it made taking transfers so much easier having the agar on the lid.

Found it

https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26467702&page=1&vc=1#26467702


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Edited by A.k.a (02/11/20 09:59 AM)


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: A.k.a]
    #26479895 - 02/11/20 10:09 AM (4 years, 6 days ago)

I’m doing them over again now. I bought the Anchor wide ones that Gizmo was talking about it, got some plastic lids for them. I just poured the agar into the jars before going in the PC. This time I poured the slightest little bit, just enough to only cover the bottom. I still had the same bubble froth stuff in my agar mix though. I’ll see how these turn out after the PC cycle.

Also my batch that I messed up - whenever I took them out of the PC this morning I see the condensation problem you guys talk about. What is the best way to get rid of this? I let the agar cool until the all the condensation cleared up before the PC, brought temperature and pressure up slow, but there was still condensation. Instead of letting them cool in the PC for a long period of time should I just take them right out after they’re done while still hot? Also, condensation will cause contaminant problems on the surface of the agar correct? So should I be turning them upside down?


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Edited by psycho_nauticus (02/11/20 10:17 AM)


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Re: Agar for a noob like me? [Re: A.k.a]
    #26480022 - 02/11/20 11:34 AM (4 years, 6 days ago)

Quote:

A.k.a said:
It works there’s a guy who just did it recently. I want to say wizard was in the name. But he said it made taking transfers so much easier having the agar on the lid.

Found it

https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26467702&page=1&vc=1#26467702



Yup thats the guy I was talking about. I still dont think it'll work with a threaded lid. Just those mini round style ones. Feel free to prove me wrong though.


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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26480072 - 02/11/20 12:12 PM (4 years, 6 days ago)

Quote:

psycho_nauticus said:
I’m doing them over again now. I bought the Anchor wide ones that Gizmo was talking about it, got some plastic lids for them. I just poured the agar into the jars before going in the PC. This time I poured the slightest little bit, just enough to only cover the bottom. I still had the same bubble froth stuff in my agar mix though. I’ll see how these turn out after the PC cycle.

Also my batch that I messed up - whenever I took them out of the PC this morning I see the condensation problem you guys talk about. What is the best way to get rid of this? I let the agar cool until the all the condensation cleared up before the PC, brought temperature and pressure up slow, but there was still condensation. Instead of letting them cool in the PC for a long period of time should I just take them right out after they’re done while still hot? Also, condensation will cause contaminant problems on the surface of the agar correct? So should I be turning them upside down?



Try bringing the heat up and down super slow instead of just slow. In the summer I dont have to do that but in the winter months I can sometimeshave worse condensation problems idk why. A little condensation wont hurt if its excessive I just pour it off in the SAB before innoculating. You could store them upside down but I've never bothered. Even when I just ignore the condensation its not much of a problem.
If you take them out right away they might come out clear but they will condensate while cooling. Condensation will also go away with time if you make the no pours and let them set around for a couple weeks.

Also you can still use the small jars too you only want to let the mycelium grow out to about the size of a US quarter before you transfer
So they'll work fine. I use 60mm petri dishes which are tiny little buggers, smaller than a regular mouth lid.


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Re: Agar for a noob like me? [Re: gizmo1]
    #26480085 - 02/11/20 12:26 PM (4 years, 6 days ago)

Quote:

psycho_nauticus said:
I’m doing them over again now. I bought the Anchor wide ones that Gizmo was talking about it, got some plastic lids for them. I just poured the agar into the jars before going in the PC. This time I poured the slightest little bit, just enough to only cover the bottom. I still had the same bubble froth stuff in my agar mix though. I’ll see how these turn out after the PC cycle.

Also my batch that I messed up - whenever I took them out of the PC this morning I see the condensation problem you guys talk about. What is the best way to get rid of this? I let the agar cool until the all the condensation cleared up before the PC, brought temperature and pressure up slow, but there was still condensation. Instead of letting them cool in the PC for a long period of time should I just take them right out after they’re done while still hot? Also, condensation will cause contaminant problems on the surface of the agar correct? So should I be turning them upside down?



Try bringing the heat up and down super slow instead of just slow. In the summer I dont have to do that but in the winter months I can sometimeshave worse condensation problems idk why. A little condensation wont hurt if its excessive I just pour it off in the SAB before innoculating. You could store them upside down but I've never bothered. Even when I just ignore the condensation its not much of a problem.
If you take them out right away they might come out clear but they will condensate while cooling. Condensation will also go away with time if you make the no pours and let them set around for a couple weeks.

Also you can still use the small jars too you only want to let the mycelium grow out to about the size of a US quarter before you transfer
So they'll work fine. I use 60mm petri dishes which are tiny little buggers, smaller than a regular mouth lid.


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26480088 - 02/11/20 12:29 PM (4 years, 6 days ago)

So when is the best time to take them out of the PC? It seems if you leave them overnight to cool, there will be a condensation problem. If you take them out too soon there will be a condensation problem while it cools out. Mine are sitting in the PC now, cycle is done, but haven't opened it up to take them out yet. At some point I need to tighten down the plastic lids on them.

If I leave them right side up, will the condensation cause contamination issues when it touches the agar? If you don't store upside down then isn't it inevitably going to drip and run down onto the agar? Are you saying store them upside down, and then when I need to open in the SAB all the condensation will just pour out?

I wish I would of known that about the transfers, then I wouldn't of thrown them away. I don't know anything about this starting off with agar, so I don't know what to expect and what not. The more and more I look up just seems to be more problems that come along. I was looking forward to inoculating my agar tonight or tomorrow, but it sounds like it will be weeks before I can when the condensation goes.


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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26480114 - 02/11/20 12:44 PM (4 years, 6 days ago)

Quote:

psycho_nauticus said:
So when is the best time to take them out of the PC? It seems if you leave them overnight to cool, there will be a condensation problem. If you take them out too soon there will be a condensation problem while it cools out. Mine are sitting in the PC now, cycle is done, but haven't opened it up to take them out yet. At some point I need to tighten down the plastic lids on them.

If I leave them right side up, will the condensation cause contamination issues when it touches the agar? If you don't store upside down then isn't it inevitably going to drip and run down onto the agar? Are you saying store them upside down, and then when I need to open in the SAB all the condensation will just pour out?

I wish I would of known that about the transfers, then I wouldn't of thrown them away. I don't know anything about this starting off with agar, so I don't know what to expect and what not. The more and more I look up just seems to be more problems that come along. I was looking forward to inoculating my agar tonight or tomorrow, but it sounds like it will be weeks before I can when the condensation goes.



Take them out as soon as the outside of the PC is cool to the touch. Sometimes condensation happens its no big deal.
Im not sayimg store them upside down I've never recommended  that.
Condensation doesnt cause contams. The concern is with moisture pooling on the surface and sloshing around contams that are there after transfering or innoculating. So if its that bad just pour it away in the SAB before use. You dont have to wait weeks but in the future you can plan ahead and make some earlier if you want.

I havent done this but I'd like to try getting one of those totes with the locking lid and gasket and using it like a desiccant chamber to clear up no pour condensation. The flow hood guys have it easy they just leave them open in front of the hood us SAB guys gotta figure it out for ourselves.

It sounds like you're getting worked up again bud just relax you are doing fine and the stuff you are stressing over is just the small details that you will figure out yourself over time. Get some spores to agar get your feet wet and have fun doing it.

Also did you really throw away the other jars?


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Offlinepsycho_nauticus
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Re: Agar for a noob like me? [Re: gizmo1]
    #26480143 - 02/11/20 01:08 PM (4 years, 6 days ago)

I did yes. I didn't know that. That's why I do stress and I know I get worked up because the small things I don't know end up affecting me in a big way. I had filled them up too much to where I wouldn't be able to see the growth properly, I was thinking I need a little more surface area also because you take from the edges of the growth. I didn't see anywhere about the quarter size transfers until you just told me. I wouldn't of thrown them away knowing that  :facepalm: 

I'm trying to look ahead and learn what's coming next, but at the same time I have to be focused on what I'm doing now. If I take in too much that's when I start feeling overwhelmed. It's also because of this that I don't want condensation to fuck everything up now. Hopefully I will get to inoculate tomorrow then? I would say tonight, but will temps still be too hot from heat in the jars and destroy my spores?

Looking ahead, I'm just going to inoculate in my SAB, and then store the jar in a cabinet somewhere correct? Nothing more than that at all? Hopefully I'm not going to come across anything else I haven't anticipated. Then during that waiting period I'll have to start researching everything to come next. Thank you and AKA for all your help and guidance and answering my questions.


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Re: Agar for a noob like me? [Re: psycho_nauticus]
    #26480441 - 02/11/20 03:13 PM (4 years, 6 days ago)

Well that sucks man I would have kept them regardless if I spent my hard earned money on them for storage if nothing else lol. Has rhe trash already been dumped? You can always grab them out if not.
They cool really fast or ahould be cool as soon as they are out of the pc I would think by tonight is a completely feasible timeline.
And yes inoculate in the SAB. Store in the cabinet or in a tote or on a shelf. It doesnt matter where you store them once inoculated.
Syringe to agar can kinda suck because of the extra moisture but it should be fine just shake the shit out of your syringe so the spores are completely distributed and put 1 or 2 drops max per dish I like to start with three dishes and pick the best out of three to transfer from but you can start with as many or few as you want.

Absolutely man like I said theres alot I dont know but im happy to help when I can. Thank you for putting up with all my grammatical errors. Im suprised you can read my posts. I have these big ol fingers and little phone. It sucks using the forums on a phone lol. Sometimes I reread what I typed and im like wtf does that say lmao. Its usually impossible to fix my typos on this phone because I cant click the right spot to edit. So I end up leaving it or getting mad and erasing entire posts.


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