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OfflineDr3
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Growth stalled on JARS - is it contam?
    #26480145 - 02/11/20 01:09 PM (3 years, 11 months ago)

Hello there! So these are PP5 plastic jars filled with oats, it comes from a G2g master which came from a MS syringe of the Golden Teacher variety. These jars are around a month and a half old, colonisation wierdly stopped on these, at the time I didn't know  this relates to bacteria, since I am still relatively new to the hobby. 

Then I found this in the jar:



so I take it they are contaminated. Now these dont show that kind of orange, but they seem unable to finish colonization,



I have to add that I did not shake these jars, they were too full to do so. As I said, I'm kinda new to this.

One further thing that worries me is that I spawned to bulk some jars that looked like the previous ones, I just tossed the few grains that were not colonised. So far, pins are starting to show, some are growing already. Should I be concerned about bacteria or anything like that?

Thanks in advance for your help and time.


Edited by Dr3 (02/12/20 04:36 PM)


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OfflineThe Mycologist
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Re: Help IDing contamination in JARS [Re: Dr3]
    #26480183 - 02/11/20 01:32 PM (3 years, 11 months ago)

Super wet looking.

Bacteria maybe. or just bad prep


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OfflineDr3
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Re: Help IDing contamination in JARS [Re: The Mycologist]
    #26480297 - 02/11/20 02:17 PM (3 years, 11 months ago)

Thank you for your answer. I boiled them for 35 or so minutes just until the first ones were bursting open. Then I strained for a couple hours and PC them for 2 hours. Likely I'm doing something wrong there.


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OfflineUpRigger
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Re: Help IDing contamination in JARS [Re: Dr3]
    #26483661 - 02/13/20 12:32 PM (3 years, 11 months ago)

I just prepared my oats about the same way, first go with oats. What do your lids look like. ✌🏻


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OfflineDr3
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Re: Help IDing contamination in JARS [Re: UpRigger]
    #26483980 - 02/13/20 03:48 PM (3 years, 11 months ago)

you can see them in one of the pictures, it's just the plastic lid totally screwed and a 0.5cm or so hole filled with polyfill to allow for some gas exchange.

I think my problem could be that a plastic membrane is  more permeable to heat and 2 hours PC cycle may be overcooking the grain. But it shouldn't affect the humidity inside the jar regardless. Maybe I should let the grain soak overnight instead of a few hours, I don't know.

But don't worry, you will probably be fine, I have had success before with that prep. I think being able to shake the jar (as in, don't fill it with grain to the top if you are doing g2g :smile: is very important.

  Then again Im not that experienced, so let's hope a more experienced cultivator can chime in : D


Edited by Dr3 (02/13/20 03:49 PM)


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