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pitriot
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PF Tek / Colonizing Temperature ?
#26479707 - 02/11/20 08:05 AM (3 years, 11 months ago) |
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Hi I am Using PF Tek method and I injected a few dozen ½ pint mason jars. I put the jars into paper bags and then all of them into a few boxes.
My apt is usually around 74 degrees. Colonization has been slow in the past (a few weeks for any sign of action, and at least 5+ weeks for full colonization). I was wondering if wrapping the boxes in a heated blanket to bring temperatures closer to 79/80 would be a good move?
Thank you
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iwh678
Budget Mycologist



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Re: PF Tek / Colonizing Temperature ? [Re: pitriot] 1
#26479749 - 02/11/20 08:31 AM (3 years, 11 months ago) |
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74 is fine. I wouldn't mess with trying to heat them.
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roarkell
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Re: PF Tek / Colonizing Temperature ? [Re: iwh678]
#26480015 - 02/11/20 11:30 AM (3 years, 11 months ago) |
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You're better off leaving them... trying to incubate them is only gonna make it easier for any contams to take over... speaking from experience... lol
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pitriot
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Re: PF Tek / Colonizing Temperature ? [Re: roarkell] 1
#26480776 - 02/11/20 06:05 PM (3 years, 11 months ago) |
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thanks for you advice. I will just leave as is and check them again at 14 days.
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Sockadin



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Re: PF Tek / Colonizing Temperature ? [Re: pitriot] 1
#26480786 - 02/11/20 06:09 PM (3 years, 11 months ago) |
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Yeah 5+ weeks is normal. The thing about heating cakes is that glass will trap the heat in the jar and the mycelium make heat as they grow.so if the outside of the jars is 76-78 your internal temperature can rise much higher and stunt growth. All the incubator teks are out dated.
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Re: PF Tek / Colonizing Temperature ? [Re: pitriot]
#26480951 - 02/11/20 07:49 PM (3 years, 11 months ago) |
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Never incubate jars. Bad practice. 74F is ideal temperature as its a tropical temp. A lot of factors can slow down colonization times, like making your substrate too wet or too dry or having spore syringes thst have low spore concentration.
5 weeks is around average. My cakes usually take about 4 weeks give or take some days.
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Nichrome
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Re: PF Tek / Colonizing Temperature ? [Re: LogicaL Chaos]
#26481153 - 02/11/20 09:54 PM (3 years, 11 months ago) |
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Whatever is comfortable for you is perfect. Room temp.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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goatchild
mr noob


Registered: 10/31/19
Posts: 162
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Re: PF Tek / Colonizing Temperature ? [Re: pitriot]
#26482012 - 02/12/20 01:32 PM (3 years, 11 months ago) |
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Quote:
RogerRabbit said:
Quote:
Seems like every spore vendor says 82-84+,
And I've been trying to correct that disinformation for years. It's all based on a chart somebody mailed to stamets many years ago showing 86F to be the peak temperature for growth of cubensis on a petri dish, and everybody just accepts it as though Moses carried it down from the mountain on a tablet of stone.
However, every single experiment I did to try to duplicate that with extremely accurate temperature monitoring was unable to verify that bogus 86F figure.
What I have repeatedly found regardless of strain, is that cubensis colonization remains rather flat from about 75F through 81F. Beginning at 83F, the rate of growth falls off sharply. By 86F, growth has slowed down nearly 50% what it was between 75f and 81F. These experiments were conducted on petri dishes that produce little to no heat because of the very thin layer of mycelium. In jars, up to several degrees of heat is produced by the colonizing mycelium, so definitely don't go over 80F to 81F if you're looking for maximum rate of growth.
Furthermore, bacteria and thermophilic molds such as Mucor, the black pin mold are stimulated by higher temperatures. Therefore using an incubator set to 86F is certainly favoring bacteria and molds, while slowing down mushroom mycelium growth.
Below is a picture of one of my colonization shelves. It sits in my bedroom at normal room temperature and quart jars of rye berries colonize fully in ten days, and pf jars colonize fully in 14 to 21 days, but usually closer to 14. How often do we see posts where people have incubators set at 86F, and they're asking why their jars aren't colonized after four to five weeks, and they have large spots of yellow liquid forming? The liquid is metabolites which the mycelium secretes in response to stress, usually from competitor molds and/or bacteria. What has happened, is they've slowed down the mycelium while stimulating the competitors.
You'll also note from the picture below, none of the jars show any signs of metabolite buildup. That's because they're colonizing very quickly at normal room temperature. RR
https://www.shroomery.org/forums/showflat.php/Number/6857581#6857581
-------------------- "What stands in the way becomes the way."
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pitriot
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Re: PF Tek / Colonizing Temperature ? [Re: LogicaL Chaos]
#26483513 - 02/13/20 11:18 AM (3 years, 11 months ago) |
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Is there an objective way to measure, to know if the water content in substrate is not too wet/dry?
Quote:
LogicaL Chaos said: Never incubate jars. Bad practice. 74F is ideal temperature as its a tropical temp. A lot of factors can slow down colonization times, like making your substrate too wet or too dry or having spore syringes thst have low spore concentration.
5 weeks is around average. My cakes usually take about 4 weeks give or take some days.
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