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InvisibleCplCauliflower
shroomyboi
I'm a teapot

Registered: 02/21/15
Posts: 135
Agar progression
    #26478412 - 02/10/20 11:28 AM (3 years, 11 months ago)

I posted recently before asking for help in making sure my agar plates are growing what I actually want them to. I'm reasonably confident that this mycelium is psilocybe cubensis.

I had one culture out of 20 grow any white myc at all, and out of that I took 15+ transfers. This "mother" culture was inoculated early Jan. Out of those transfers, I probably transferred 7 more plates out of that, those transfers were done on the 29th of Jan. So currently I have 7 "master" plates and each of those plates has 4+ transfers cut out of them. My plan is for the master plates to become grain jars and the transfers to inoculate liquid cultures once I deem them clean enough.

Anyways, onto the pictures. Labels are formatted as
[Date transferred]
[Master plate # and transfer letter]

I did mislabel one or two but fuck it, i was stoned :shrug:
The important part is that I can isolate some good genetics for future grows. I can get some grown and take clones/prints all day, but i've been struggling for the past few weeks isolating out of a very dirty few spore syringes.

This is where it all started and what she looks like now


One isolate



Another




Another




Here are some other pics










Comments, questions, and advice welcome. I'm 100% ready to learn something


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OnlineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 9 minutes, 43 seconds
Trusted Cultivator
Re: Agar progression [Re: CplCauliflower]
    #26478639 - 02/10/20 01:54 PM (3 years, 11 months ago)

Nice man you came a long way from that first plate.

They look good especially that 5 plate.

I just recently started up with lc myself. Using it to make a small jar of grain and then g2ging that is super fast for getting larger amounts of spawn.


--------------------
LAGM2020


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InvisibleCplCauliflower
shroomyboi
I'm a teapot

Registered: 02/21/15
Posts: 135
Re: Agar progression [Re: A.k.a]
    #26478750 - 02/10/20 03:15 PM (3 years, 11 months ago)

Thanks! Been very worried I'm wasting my time but now the plates are telling me I've got something workable here. I want to make these plates the stepping stone I can branch out from and begin the cycle of making all of my own culture and spores - I'm getting sick of dealing with inadequate spore syringes.


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Invisiblerm1024
Familiar Stranger
Registered: 11/05/18
Posts: 62
Re: Agar progression [Re: CplCauliflower]
    #26478834 - 02/10/20 03:57 PM (3 years, 11 months ago)

I really appreciate being able to use plates to make sure I'm not wasting grainspawn on shit cultures.  It's just one less thing to worry about when you put them to grain.  You know exactly whats going in and if it goes to shit you know its your grain prep or your lc prep.  Like saving a spot in a videogame instead of having to start over is how I look at it.


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
Trusted Cultivator
Re: Agar progression [Re: rm1024]
    #26478842 - 02/10/20 04:02 PM (3 years, 11 months ago)

Are they all from the same culture? They look really good bro.


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InvisibleCplCauliflower
shroomyboi
I'm a teapot

Registered: 02/21/15
Posts: 135
Re: Agar progression [Re: Sockadin]
    #26478847 - 02/10/20 04:04 PM (3 years, 11 months ago)

The plates make it so easy to compare cultures, and to whittle down 17 cultures into just the best performing 3 or 4 groups.

@Sockadin Yes every single one of the plates I've posted this month have come from the single infected culture you see in my first picture. I'm currently two transfers deep in this family tree and I'm planning #3 for tomorrow or the next day.


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Invisiblerm1024
Familiar Stranger
Registered: 11/05/18
Posts: 62
Re: Agar progression [Re: CplCauliflower]
    #26479152 - 02/10/20 07:17 PM (3 years, 11 months ago)

17?  wow.  i usually work 3 at a time and take 3 from the best.  Id have a zillion otherwise.  I use glass dishes for the most part tho and hate doing glassware. :smile:


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InvisibleCplCauliflower
shroomyboi
I'm a teapot

Registered: 02/21/15
Posts: 135
Re: Agar progression [Re: rm1024]
    #26479710 - 02/11/20 08:06 AM (3 years, 11 months ago)

Yeah I went a little crazy because I really wanted to make sure I got a good culture out of it - I mean look at how dirty that plate is that they all came from! I probably got the dirtiest spore syringes in north america. I'm definitely not going to keep transferring all of them, there are some cultures that are too wispy and fuzzy while I have 4 or so cultures I'm actually looking at proliferating.


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