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OfflineInfinity-25
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Starting up: Growlog. Clones to agar to rye to shoebox - comments please
    #26478087 - 02/10/20 08:04 AM (3 years, 11 months ago)

Dear shroomery community.

First of all, thank you for kicking ass. I learned loads just by reading the shroomery over the past few months. Now I would like your input/comments on my efforts on starting up a Cubensis grow. OK so here goes:


23-12-19

Pouring agar plates. Recipe 400ml water, 8g agar, 8g malt extract, 0.8g yeast
Pouring was succesfull, no contams on plates, although a lot of condensation probably due to pouring too hot.

24-12-19

Inoculated a few plates with a 7-8 year old spore syringe. Fail, no myc, only bacteria.

27-12-19

Decided to buy a growkit (P. Cubensis, Golden teacher) from online smartshop (I live in NL :wink: ) Soaked for 12h and put into fruiting conditions. 

Reason for growkit being I want to start up quickly. So opting for cloning rather than spores for the time being. I would like to clean up my own cultures later from good flushes.

21-1-20

First flush of growkit. Picked the 2 biggest shrooms and put them to agar. I used tissue from inside the stem of the shrooms. Working in SAB, flame sterilize scalpel between each plate.


Growkit 1st flush


1 of the clones, 2 days after transfer

27-1-20

Transfer to 2 new plates from 1 of the clone plates.


2 days after transfer

Also made 2 more clone plates just to make sure I spread my chances
Here is how the plates look after 2 weeks:





Seems like healthy growth to me. Obviously some contams on the 2nd plate here. I read to not transfer clones too often before going to grains. What do you guys recommenend to do with these plates? Clean/transfer more?

31-1-20

Rye jar sterilisation. I used RR's tek mainly: Rinsed the grains a few times until water is clear, soak for 6h, boil 10 mins, let evaporate in coriander for 30-45mins. Once steam subsided, loaded up 6 jars for a trial run. PC for 2h @15psi (started with 10min venting as recommended). Cooled over night.


Loading the jars in my brand new 23qt Presto <3

1-2-20

Knocked up jars from 2 different petris, as there is some myc growing here (CAA02 and CAB01)



Also made 1 control jar to see if PC is succesful (no inoculation, unopened)


Rye jars 1 day after inoculation


One of the jars, 2 days after inoculation. Myc is starting to reach for the grains.



5 days after inoculation (with/without flash)


5 days after inoculation, closeup of 1 of the jars


9 days after inoculation, same jar.


I put the in a dark room where our boiler is, so its around 20-24C. Not shaken yet.

3-2-20

In addition, I knocked up a few smaller jars with a slightly bigger gas exchange hole (about 1cm, stuffed with polyfill, the previous jars were 0.6-0.7 cm holes). I also put in a little bigger piece of colonized agar from these jars, hoping colonisation will be faster here. I did use the same plates as for the previous jars as there was no visible contamination. May be a mistake though..


small jars, 7 day after inoculation


Close-up, 7 day after inoculation


Close-up, 7 day after inoculation


What do you guys think? Do the jars look healthy to you? I dont have much experience yet with grains and it looks somewhat dry to me.

What about colonisation speed? Should I shake already? The 9d and 7d pics are from today.

Thanks for your comments. I will keep updating this thread as time goes by. Hopefully can knock up some shoe boxes soon. Im planning to to some G2G first from a few of the most healthy looking and fully colonized jars.


Edited by Infinity-25 (02/10/20 12:59 PM)


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OfflineInfinity-25
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Registered: 04/07/10
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Re: Starting up: Growlog. Clones to agar to rye to shoebox - comments please [Re: Infinity-25]
    #26478552 - 02/10/20 01:01 PM (3 years, 11 months ago)

*Edited with formatted pictures*

Any comments on my progress?


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