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vinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
Posts: 492
Last seen: 8 months, 2 days
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High pressure co2 and n2o sterilization
#26477662 - 02/09/20 09:15 PM (3 years, 11 months ago) |
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So I've been reading about high pressure. Carbon dioxide sterilisation. They also use nitrous oxide, but it does not work as well. So I filled up this whipped cream dispenser With 24-hour soaked WBS. After 20 minutes I opened it up , and all the bird seed is fizzing Like I opened a can Of Coke. Thoughts? Do I have to let this gas disperse before I throw it in a jar and inoculate? I'm thinking about Not pressure cooking one jar just to see how good the sterilization process works. , even though I'm using n2o and not CO2. What do you guys think of this? ?
Also, if the psi of the 8 gram n2o cartridge is 400 as they say, Is it possible to do the math and find out the PSI of that cartridge when it's emptied into a Container with a 500 mL volume?
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Sir Pentinite
Stranger all the time.

Registered: 05/15/19
Posts: 525
Loc: ation Location Location
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Re: High pressure co2 and n2o sterilization [Re: vinnie boombotz]
#26477757 - 02/09/20 10:59 PM (3 years, 11 months ago) |
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Nitrous oxide (N2O) isn't used for sterilization, nitrogen dioxide (NO2) is. Unfortunately you're not going to sterilize anything with whippits.
Quote:
vinnie boombotz said: Also, if the psi of the 8 gram n2o cartridge is 400 as they say, Is it possible to do the math and find out the PSI of that cartridge when it's emptied into a Container with a 500 mL volume?
Yes. Have a look at the combined gas law equation.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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vinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
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Re: High pressure co2 and n2o sterilization [Re: Sir Pentinite]
#26477802 - 02/09/20 11:45 PM (3 years, 11 months ago) |
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Anyways, I think I fucked them up already. It said rapid Decompression ruptures the cells and spore walls. So i released the pressure real quick and I heard a bunch of seeds go pop.
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MH5109
Stranger


Registered: 09/27/17
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Re: High pressure co2 and n2o sterilization [Re: vinnie boombotz]
#26477816 - 02/10/20 12:02 AM (3 years, 11 months ago) |
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Really just from decompression. I literally know nothing about what you guys are talking about but i just find it hard to believe a moist seed hull would make much of a sound if it ruptures, not saying you are wrong it just seems weird to me. It would be cool to see if it works, hell 20 min sterilization that would be awesome lol.
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JohnRainyII
Unapproved Puppet

Registered: 02/07/20
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Re: High pressure co2 and n2o sterilization [Re: vinnie boombotz]
#26477818 - 02/10/20 12:04 AM (3 years, 11 months ago) |
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What the hell?
The spores are in there already?
Im confused.
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jcm4620
Stranger


Registered: 05/26/19
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Re: High pressure co2 and n2o sterilization [Re: JohnRainyII]
#26477828 - 02/10/20 12:11 AM (3 years, 11 months ago) |
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lol sounds like a waste of good nitris lol god i use to love them lil canisters we use to be able to buy them by the case here at the head shop til they got busted lol 25 boxes of 24 canisters lol 600 whipits for 250$ = a fun night🤪🤪
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vinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
Posts: 492
Last seen: 8 months, 2 days
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Re: High pressure co2 and n2o sterilization [Re: jcm4620]
#26477854 - 02/10/20 12:53 AM (3 years, 11 months ago) |
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Quote:
JohnRainyII said: What the hell?
The spores are in there already?
Im confused.
The endospores I meant
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jcm4620 said: lol sounds like a waste of good nitris lol god i use to love them lil canisters we use to be able to buy them by the case here at the head shop til they got busted lol 25 boxes of 24 canisters lol 600 whipits for 250$ = a fun night🤪🤪
I used to like whippets when I was a kid. But now it feels like it's trying to kill me, and not in a good way.
I'm going to grab some CO2 cartridges tomorrow And try those
Edited by vinnie boombotz (02/10/20 12:57 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: High pressure co2 and n2o sterilization [Re: vinnie boombotz]
#26477964 - 02/10/20 04:46 AM (3 years, 11 months ago) |
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Co2 pressure sterilization uses pressures in the thousands of PSI.
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vinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
Posts: 492
Last seen: 8 months, 2 days
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Re: High pressure co2 and n2o sterilization [Re: bodhisatta]
#26478003 - 02/10/20 06:02 AM (3 years, 11 months ago) |
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Hmm... Maybe I'll get a hamster instead.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: High pressure co2 and n2o sterilization [Re: vinnie boombotz]
#26478041 - 02/10/20 07:05 AM (3 years, 11 months ago) |
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Yea 27.5 mpa is nearly 4000psi lmao.
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SynKyd
ctrl-alt-delite



Registered: 09/27/13
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Re: High pressure co2 and n2o sterilization [Re: vinnie boombotz]
#26478069 - 02/10/20 07:40 AM (3 years, 11 months ago) |
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Quote:
vinnie boombotz said:

Anyways, I think I fucked them up already. It said rapid Decompression ruptures the cells and spore walls. So i released the pressure real quick and I heard a bunch of seeds go pop.
But did you get the full rip on the whip it or was it also degraded but the WBS?
Lol
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