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Amazon Shop: ½ Pint Jars, Pressure Cooker

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OfflineDrMambo
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Listerine, anyone?
    #2647711 - 05/06/04 10:47 PM (13 years, 3 months ago)

Posting this for a foaf:

I just sterilized 6 PF jars which are going to sit in the PC overnight to cool to be innoculated tommorow morning. The problem is (and I realized this only after starting the sterilization) that I don't have any rubbing alcohol. I'm thinking, would it work to heat the tips until glowing, wiping with a swab in listerine, then innoculating? Or, would the jars be ok sitting in the PC all day until about 4:00 when I can use rubbing alcohol to sterilize the syringes?


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"Yeah, he's a professor...... OF BEING A DOG!"


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Offlineyeehaw
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Registered: 05/05/04
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Re: Listerine, anyone? [Re: DrMambo]
    #2647724 - 05/06/04 10:50 PM (13 years, 3 months ago)

Why do you need to wipe it? When I do mine I never wipe after flaming. I use my gas kitchen stove and just wait about 2 minutes and then let a drop out of the end. I just wouldnt wipe... as long as you are using a clean burning flame


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OfflineDNICEONER
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Registered: 04/03/04
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Re: Listerine, anyone? [Re: yeehaw]
    #2647737 - 05/06/04 10:53 PM (13 years, 3 months ago)

you NEED to wipe... may work sometimes without it, but you gotta be safe or risk disaster for every jar innoculated with that potentially contaminated syringe....

Listerine is only what, 40-50% alcohol? its got several other active ingredients. Don't mess with it. If your a beginner don't deviate from the proven methods....

Your jars will be perfectly FINE inside the cooker until 4 tomorrow...... just wait.


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OfflineDrMambo
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Re: Listerine, anyone? [Re: yeehaw]
    #2647743 - 05/06/04 10:56 PM (13 years, 3 months ago)

I won't be able to heat the entire length of the syringe with just heat which would cause unsterile portions to get into the cake.


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"Yeah, he's a professor...... OF BEING A DOG!"


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InvisibleOldSpice
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Re: Listerine, anyone? [Re: DrMambo]
    #2647746 - 05/06/04 10:56 PM (13 years, 3 months ago)

Its better than nothing


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So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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OfflineGr0wer
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Re: Listerine, anyone? [Re: OldSpice]
    #2647753 - 05/06/04 10:59 PM (13 years, 3 months ago)

run out to a local 7/11,grecery, or drug store its $1 for a bottle of rubbing alcohol.


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OfflineDNICEONER
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Re: Listerine, anyone? [Re: DrMambo]
    #2647806 - 05/06/04 11:11 PM (13 years, 3 months ago)

why can't you? you don't have a lighter? you have to apply flame AND wipe down with alcohol.
There wil be black residue left over from the flame after wiping with alcohol, but it will be clean.


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InvisibleButterNut
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Registered: 04/17/04
Posts: 1,466
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Re: Listerine, anyone? [Re: DrMambo]
    #2647809 - 05/06/04 11:10 PM (13 years, 3 months ago)

Quote:

DrMambo said:
Or, would the jars be ok sitting in the PC all day until about 4:00 when I can use rubbing alcohol to sterilize the syringes?



yes thats fine.


--------------------
Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.


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Offlineyeehaw
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Re: Listerine, anyone? [Re: DrMambo]
    #2647931 - 05/06/04 11:34 PM (13 years, 3 months ago)

I still don't understand why yall are wiping the syringe. That seems like it would have more of a chance to become contaminated. There is NO POSSIBLE WAY the syringe can be contaminated if you heat it until red hot... there is no bacteria on it. I am a microbiology student and in labs we NEVER touch our innoculation loops with ANYTHING after heating until they are red hot... it seems like wiping with a towel in alchohol would introduce contams because alcohol does not kill all microorganisms. Not trying to flame anyone... just adding my take on it


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InvisibleOldSpice
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Re: Listerine, anyone? [Re: yeehaw]
    #2647953 - 05/06/04 11:36 PM (13 years, 3 months ago)

I just wipe with alchohol
..


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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InvisibleButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
Re: Listerine, anyone? [Re: yeehaw]
    #2647956 - 05/06/04 11:36 PM (13 years, 3 months ago)

I dont flame the needle at all.I just use an alchahol prep pad,and ve never had a problem.In between jars if i have to set it down,i just fold the prep pad around the needle.


--------------------
Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.


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InvisibleOldSpice
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Re: Listerine, anyone? [Re: ButterNut]
    #2647974 - 05/06/04 11:39 PM (13 years, 3 months ago)

Yeah and i just use toilet paper soaked good...no flame


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Listerine, anyone? [Re: ButterNut]
    #2647975 - 05/06/04 11:39 PM (13 years, 3 months ago)

Yeah and i just use toilet paper soaked good...no flame


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleTremor1127
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Registered: 08/10/03
Posts: 3,404
Loc: In a Van Down By the Rive...
Re: Listerine, anyone? [Re: yeehaw]
    #2647994 - 05/06/04 11:40 PM (13 years, 3 months ago)

you have to wipe it off because there is black residue left over that you dont want to get everywhere... and what better thing to do it with than alchohal... and who cares if your a microbiology student in labs, im sure your professor who isnt just book smart but knows his shit will tell you that rubbing with alchohal is not a harmful thing...


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InvisibleButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
Re: Listerine, anyone? [Re: OldSpice]
    #2648020 - 05/06/04 11:43 PM (13 years, 3 months ago)

Quote:

Alounacara said:
Yeah and i just use toilet paper soaked good...no flame



you do not.


--------------------
Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.


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InvisibleTremor1127
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Re: Listerine, anyone? [Re: Tremor1127]
    #2648024 - 05/06/04 11:43 PM (13 years, 3 months ago)

and yes, alchohal prep pads alone work wonders... flaming the tip once just before first use is a + though, not nescessary though...


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Offlineyeehaw
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Registered: 05/05/04
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Re: Listerine, anyone? [Re: Tremor1127]
    #2648043 - 05/06/04 11:47 PM (13 years, 3 months ago)

there is no black residue if you use a clean burning flame (not a cigarette lighter) But... I just talked to my roomate who works in surgery and he told me that wiping with alchohol wouldn't hurt anything. So I stand corrected... but I wouldn't trust just alcohol.


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InvisibleOldSpice
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Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Listerine, anyone? [Re: yeehaw]
    #2648056 - 05/06/04 11:49 PM (13 years, 3 months ago)

Been doing it that way a couple of years


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
Re: Listerine, anyone? [Re: yeehaw]
    #2648060 - 05/06/04 11:50 PM (13 years, 3 months ago)

ive done it the way i do it for 7 years now and ive probably only had a dozen jars contam do to innoculation.I trust it just fine.


--------------------
Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.


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InvisibleOldSpice
Geritol Breath...
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Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Listerine, anyone? [Re: ButterNut]
    #2648068 - 05/06/04 11:51 PM (13 years, 3 months ago)

Ya gonna vote for me tomorrow... Me vs BF6 :cool:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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