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OfflineInsultingLizard
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Too many spores?
    #26477069 - 02/09/20 02:54 PM (3 years, 11 months ago)

I know this has been asked before, but is it possible to inject so many spores into a substrate that it just doesn't colonize? I got three prints from a spore ring. Two of the prints were so tiny and thin that I decided to only make one syringe for each, but the third one was a big and dense Oak Ridge print. I guess I fucked up and made only two super loaded syringes; there were visible specks of spores floating in the water. The three jars I inoculated with those syringes are the only ones that haven't colonized in the least after 12 days.

So, first: did I fuck up, or is it more likely that the spores just weren't viable?
Second: How long do you wait before calling it quits and tossing an uncolonized jar?


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Offlineiwh678
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Re: Too many spores? [Re: InsultingLizard]
    #26477150 - 02/09/20 03:59 PM (3 years, 11 months ago)

Too many spores can cause other problems, but it wont stop them from germinating. It could be that the print was old.

You could try a few methods for germinating old spores on agar.


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OnlineNichrome
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Re: Too many spores? [Re: iwh678]
    #26477296 - 02/09/20 05:22 PM (3 years, 11 months ago)

Almost all prints have other organisms present. The more spore you use the more likely contams will be present/flourish. You will also have a larger genepool and more variety of phenotypic expression of fruits. Lots of spores doesn't affect whether or not you get a full canopy as much as environment does.

I like to use lots of spores with agar and sector out patterns until each is on it's own. They seem to meet and bond out of choice rather than necessity and form better fruits using lots of spores. It is a bigger job to separate them into colonies but it's worth it IMO...:heart:


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OfflineInsultingLizard
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Re: Too many spores? [Re: Nichrome]
    #26479118 - 02/10/20 06:58 PM (3 years, 11 months ago)

Alright guys, thanks. I figured it was the print. One last question: if you wanted to try out a print you received for viability, would you test it on agar first, or would you inoculate directly (assuming you don't always start with agar)?


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Offlineiwh678
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Re: Too many spores? [Re: InsultingLizard]
    #26479140 - 02/10/20 07:09 PM (3 years, 11 months ago)

Yeah you pretty much always start with agar.


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OfflineInsultingLizard
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Re: Too many spores? [Re: iwh678]
    #26479146 - 02/10/20 07:14 PM (3 years, 11 months ago)

What if you're doing PF cakes, though? How do you inoculate a cake with an agar slice? Wouldn't you have to take out the verm filter first?


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Offlineiwh678
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Re: Too many spores? [Re: InsultingLizard] * 1
    #26479184 - 02/10/20 07:35 PM (3 years, 11 months ago)

You can either do a liquid inoculation which is basically a slurry made from and agar plate or you could make a liquid culture.


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OfflineInsultingLizard
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Re: Too many spores? [Re: InsultingLizard] * 1
    #26479211 - 02/10/20 08:03 PM (3 years, 11 months ago)

Thanks, that was the bit of info I was missing. Actually I should have checked the flowchart on getting started, it was all there. For my next grow this week or the next I'll definitely start with agar.


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