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shadowpuppets
Stranger
Registered: 11/26/19
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Agar Contamination: Can I Isolate? Pls Help Me Keep Growing
#26470502 - 02/05/20 03:27 PM (4 years, 12 days ago) |
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Hello folks,
I am a first time grower. I had a grow kit and inoculated the growbag with a multispore syringe (Cambodia). It is now in fruiting conditions and I am waiting to get some shrooms.
I don’t have access to grow kits, spore syringes/prints etc. so I am trying to find a way to continue growing. As a first step, I decided to go from grain spawn to agar just before taking the grow bag to fruiting conditions. So I used flame sterilized tweezers to pick a rye grain covered with mycelium and put it into the agar plate (BRF) in my SAB. I made 10 agar dishes 11 days ago. It seems to me that most of them are contaminated, I hope I am mistaken. Please see the pictures, I will try to sort them from the worst to best looking. My question is: which ones are the best to make isolation and when? Which ones should I toss? You can refer the pics by number 1-10. Sorry for the condensation, those are noobly poured agar dishes.
Day 11:

Contamination could be from PC cycle or when pouring agar. I didn’t have the time to wait for a week after pouring agar to see if the agar plates are contaminant free.
With the purpose of continuing growing, my plan is to try to make spore print + clone a tissue from the best looking shroom when my cake from the grow bag fruits. Since I can't obtain any other grow kits, this is my last chance. So if I can’t isolate mycelium from these agar plates, and if my cake fails, unfortunately I have to say “farewell” to shrooms and shroomery So please help me out. What else can I do? Any recommendation would be very helpful!
Note: This is my first post. I've been reading the forum a lot lately. I want to thank you all for the beautiful information and help. Wish I had the information I have now, before inoculating the whole syringe into the growbag.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,755
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Re: Agar Contamination: Can I Isolate? Pls Help Me Keep Growing [Re: shadowpuppets]
#26470577 - 02/05/20 04:07 PM (4 years, 12 days ago) |
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I'd transfer from the bottom/rt area of the last 3 plates. If those fail, you can always take clones from your current grow.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
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Re: Agar Contamination: Can I Isolate? Pls Help Me Keep Growing [Re: LadysKnight]
#26470586 - 02/05/20 04:11 PM (4 years, 12 days ago) |
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You’ve got plenty of good growth on the plates you should be fine.
Hopefully you get at least one shroom to print soon so you’ll be set.
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LAGM2020     
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shadowpuppets
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Registered: 11/26/19
Posts: 35
Last seen: 3 years, 3 months
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Re: Agar Contamination: Can I Isolate? Pls Help Me Keep Growing [Re: A.k.a]
#26475251 - 02/08/20 10:19 AM (4 years, 9 days ago) |
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Quote:
A.k.a said: You’ve got plenty of good growth on the plates you should be fine.
Hopefully you get at least one shroom to print soon so you’ll be set.
Thanks man, I hope so. I will update this thread when I am hopefully successful. I really want to continue growing!
Quote:
LadysKnight said: I'd transfer from the bottom/rt area of the last 3 plates. If those fail, you can always take clones from your current grow.
Okay great. But when should I do the transfers? And from those 3 plates to how many plates?
Quote:
Stropharis said: You would ideally want to do a transfer from a dish as soon as you see growth to avoid spreading any contaminants during the transfers. The less there is growing on your dish before the transfer the better.
Stro says that it is best to do a transfer asap, but I made 10 new agar dishes couple days ago and want to wait for a week to see if they are contaminant free. Do you think that would be huge problem considering my colonized dishes?
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 hours, 48 minutes
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Re: Agar Contamination: Can I Isolate? Pls Help Me Keep Growing [Re: shadowpuppets]
#26475263 - 02/08/20 10:32 AM (4 years, 9 days ago) |
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No a week is fine. You could always take a couple now so that if the plates are bad you won’t lose much and if they’re good you save a week.
Unless there’s visible contams then I’d transfer ASAP. Otherwise it usually takes a few weeks to grow out to the edge
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LAGM2020     
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WeavieWonder
Time Travel Sucks



Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 1 month, 22 days
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Re: Agar Contamination: Can I Isolate? Pls Help Me Keep Growing [Re: A.k.a]
#26475337 - 02/08/20 11:27 AM (4 years, 9 days ago) |
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8 and 10 8 are your best bet. Take transfers anywhere from 3 - 6 o'clock for both.
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